Article
Immunology
Wenjie Guo, Rui Han, Weizhen Xu, Zijun Lu, Yanwei Li, Xueming Dan, Zequan Mo
Summary: This study investigated the immune protective effects of an inactivated F. columnare vaccine. The results showed that when white oil adjuvant was used as a booster, the relative percent survival (RPS) after 8 weeks of vaccination was 34% and 61%, respectively. Furthermore, the inactivated vaccine comprising an appropriate antigen isolate with white oil adjuvant as a booster can provide effective protection in grass carp.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Nan Wang, Jiahao Li, Yajun Wang, Yingying Wang, Defeng Zhang, Cunbin Shi, Yingying Li, Sven M. Bergmann, Xubing Mo, Jiyuan Yin, Qing Wang
Summary: In this study, Lactococcus lactis was used as an oral vaccine delivery system to express the VP6 protein of grass carp reovirus II (GCRV II). It was found that the recombinant L. lactis strain could induce mucosal immune protection, enhance nonspecific immune responses, and elicit both systemic and mucosal immunity in fish. This study suggests that L. lactis is a promising candidate for oral vaccine delivery in the aquaculture industry.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
De-Kui Qiu, Yan Xiong, Yu-Ming Gong, Yu-Ying Zheng, Rui Ma, Chen Zhang, Gao-Xue Wang, Bin Zhu
Summary: The study found that grass carp vaccinated with pcDNA3.1-vp4 showed better immune responses compared to pcDNA3.1-vp4-3, with the latter having a higher survival rate in preventing GCRV infection.
Article
Fisheries
Kai Hao, Yu Wang, Bin Zhu, Fei Yu, Zhe Zhao, Gao-Xue Wang
Summary: This study demonstrates that surface display of GCRV core antigen vaccine and invasion component from aquatic pathogenic microorganism on nonpathogenic Escherichia coli can effectively prevent GCRV viral diseases via the immersion administration approach.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Yuan Yuan Yang, Si Yao Zheng, Hong Fang, Xiao Man Wu, Jie Zhang, Ming Xian Chang
Summary: This study investigated the effects of different variants of histone H2A in grass carp on Flavobacterium columnare infection. It was found that some variants had antibacterial activity against F. columnare and could enhance the innate immune responses in grass carp. Grass carp immunized with these variants showed lower mortality and fewer numbers of F. columnare. These findings suggest that histone H2A variants play an important role in enhancing disease resistance in fish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Zijun Lu, Ren Gao, Yafei Duan, Rui Han, Wenjie Guo, Xueming Dan, Yanwei Li
Summary: This study isolated 45 bacterial strains of F. columnare from grass carp in various provinces of China, identifying three genotypes with genomovar I being the dominant type. Analysis revealed genetic differences among the three genomovars, with a closer relationship between genomovar I and II. The study provides insight into the genetic diversity of F. columnare in Chinese grass carp.
Article
Fisheries
Guojing Xu, Jinlu Zhang, Rufang Ma, Chao Wang, Huizhong Cheng, Junxia Gong, Zhizhong Wang, Qinglei Meng
Summary: The study demonstrated that both bath and intraperitoneal immunization up-regulated pIgR and secretory Ig expression in secretions, providing more insights into the role of pIgR in immunity and offering insight into ways of protecting teleost against pathogen invasion.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Changyong Mu, Qiwang Zhong, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Yiqun Li, Mingyang Xue, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: In this study, a recombinant baculovirus expressing VP35-VP4 proteins from GCRV was generated and used to vaccinate grass carp through oral administration. The results showed that the vaccine induced both non-specific and specific immune responses, providing protection against GCRV infection with a relative percent survival rate of 56%. This study suggests that the silkworm pupae powder could be used as an effective oral vaccine for grass carp.
Article
Genetics & Heredity
Hui Wang, Xi Chen, Sisi Li, Chengchong Zhou, Lili Xu, Zhixin Wu, Xiaoxuan Chen
Summary: Langerhans cells (LCs) in grass carp were identified for the first time using immunofluorescence and transmission electron microscopy, and a CD207 gene similar to human and mouse counterparts was discovered. The CD207 gene was found to be highly expressed in immune organs and played a significant role in the immune response to bacterial infections.
Article
Microbiology
Paul D. D. Veith, Dhana G. G. Gorasia, Eric C. C. Reynolds
Summary: In this study, the O-glycoproteome of F. johnsoniae was characterized and 325 O-glycosylation sites were identified among 226 glycoproteins. The glycosylated domains were mainly located in the periplasm, which is believed to be beneficial for protein folding and stability. Additionally, six proteins associated with gliding motility or the T9SS were shown to be O-glycosylated.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Microbiology
Todd J. Eckroat, Camillus Greguske, David W. Hunnicutt
Summary: Flavobacterium johnsoniae forms biofilms in low nutrient conditions, with the T9SS secretion system playing a crucial role in biofilm formation. Research shows that a functional T9SS is necessary for biofilm formation, while gliding motility, although not essential, contributes to the formation of a robust biofilm.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Ting Gao, Caixia Gao, Siyu Wu, Yingying Wang, Jiyuan Yin, Yingying Li, Weiwei Zeng, Sven M. Bergmann, Qing Wang
Summary: The study successfully developed GCRV II-based virus-like particles (VLPs) using a bac-to-bac expression system, which demonstrated excellent immunogenicity and showed protective effects in grass carp after immunization.
Article
Fisheries
Hehe Xiao, Shengran Yun, Wenji Huang, Huifeng Dang, Zhao Jia, Kangyong Chen, Xin Zhao, Yaxin Wu, Yanjie Shi, Junya Wang, Jun Zou
Summary: Recombinant CiIL-4/13A and CiIL-4/13B proteins were produced, and monoclonal antibodies against these proteins were prepared. The CD3?/d(+)/IL-4/13A(+) and CD3?/d(+)/IL-4/13B(+) cells were found to be present in the gills, intestine, and spleen, and their expression was upregulated after Flavobacterium columnare infection. These cells play an important role in maintaining homeostasis and regulating mucosal immunity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Keiko Sato, Masami Naya, Yuri Hatano, Yoshio Kondo, Mari Sato, Yuka Narita, Keiji Nagano, Mariko Naito, Koji Nakayama, Chikara Sato
Summary: The colony spreading of Flavobacterium johnsoniae is influenced by gliding motility, the cell surface adhesin SprB, as well as agar and glucose concentrations. In the presence of glucose, two distinct phases were observed in colony spreading: an initial cell growth-dependent phase followed by a secondary SprB-independent, gliding motility-dependent phase. The patterns of colony branching and cell movement were found to be different between the wild-type and mutant strains.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Lei Zhu, Gaoliang Yuan, Xinru Wang, Tong Zhao, Libo Hou, Chen Li, Xinyu Jiang, Jie Zhang, Xianliang Zhao, Chao Pei, Li Li, Xianghui Kong
Summary: In this study, a vaccine delivery system using duckweed as a host for the expression of the S11 segment of grass carp reovirus (GCRV) was successfully constructed. The expression of the S11 segment in transgenic duckweed was verified using various methods, including GUS staining, PCR, Western blotting, and fluorescence observation. The results showed that the expression vector was successfully transferred and replicated in duckweed, and a fusion protein with a molecular mass of approximately 50 kDa was expressed. This study provides valuable information for the development of an edible vaccine against GCRV in grass carp.
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.