Article
Plant Sciences
Paige E. Panter, Jacob Seifert, Maeve Dale, Ashley J. Pridgeon, Rachel Hulme, Nathan Ramsay, Sonia Contera, Heather Knight
Summary: We demonstrate the importance of fucose-dependent pectin cross-linking in stomatal development and leaf water loss control. The reduction of guard cell wall stiffness in a fucosylation-defective mutant does not affect stomatal closure. The fucosylation levels of cell walls and freezing tolerance of the Arabidopsis sensitive-to-freezing8 (sfr8) mutant are compromised. Leaf water loss is significantly higher in sfr8 and the allelic mutant mur1-1, indicating that CW fucosylation affects the response to desiccation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Oleksandr Zhdanov, Michael R. Blatt, Hossein Zare-Behtash, Angela Busse
Summary: The study found that Arabidopsis has a positive anemotropic response to constant unidirectional wind, resulting in significant alterations to the plant's morphology, mechanical properties, and anatomical tissue organization. This suggests that the plant's response to different types of mechanical stimuli may vary.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Multidisciplinary Sciences
Hongcheng Zhao, Nan Sun, Lin Huang, Ruyi Qian, Xianyong Lin, Chengliang Sun, Yongguan Zhu
Summary: The molecular mechanism of root development mediated by the beneficial bacterium Azospirillum brasilense is not fully understood. A. brasilense induces extensive transcriptional changes but inhibits primary root elongation in Arabidopsis. Through analysis of root cell type-specific markers, it was found that A. brasilense does not alter the overall organization or cell division of the primary root meristem. The inhibition of root elongation is primarily due to the reduction of cell elongation, which is caused by bacterially activated peroxidase leading to cell wall cross-linking. The activation of peroxidase, in combination with the downregulation of cell wall loosening enzymes, results in an increase in cell wall thickness.
Article
Engineering, Biomedical
Jacob Seifert, Charlotte Kirchhelle, Ian Moore, Sonia Contera
Summary: The shapes of living organisms are formed and maintained by precise control in time and space of growth, achieved by dynamically fine-tuning the mechanical properties of their structures. The study demonstrates the continuity of simple, linear, viscoelastic behavior of cell walls across scales, with characteristic nanometer scale patterns of relaxation times, elasticity and viscosity correlating with the speed of macroscopic growth. The approach provides a method to quantitatively map energy stored, dissipated and time response of cell walls in living organisms with nanometer resolution.
ACTA BIOMATERIALIA
(2021)
Article
Acoustics
Ziga Pandur, Matevz Dular, Rok Kostanjsek, David Stopar
Summary: This study systematically investigated the resistance of bacterial cells to ultrasonic irradiation by altering the material properties of E. coli cells. The results showed that peptidoglycan, a major component of the cell wall, was the most important determinant of bacterial resistance to sonolysis. Cells without peptidoglycan exhibited high sensitivity to sonolysis.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Rui Han, Waldemar Vollmer, John D. Perry, Paul Stoodley, Jinju Chen
Summary: In this study, the mechanical properties and turgor of bacterial cells were determined simultaneously using atomic force microscopy (AFM) nanoindentation. The results showed that the cell wall stiffness of bacteria increased linearly with an increase in turgor, and higher osmolarity led to a decrease in both cell wall stiffness and turgor.
Article
Biochemistry & Molecular Biology
Haiyue Ai, Julia Bellstaedt, Kai Steffen Bartusch, Lennart Eschen-Lippold, Steve Babben, Gerd Ulrich Balcke, Alain Tissier, Bettina Hause, Tonni Grube Andersen, Carolin Delker, Marcel Quint
Summary: Roots are able to sense and respond to elevated temperature independently of shoot-derived signals. This response is mediated by an unknown root thermosensor that uses auxin as a messenger to relay temperature signals to the cell cycle. Growth promotion is primarily achieved by increasing cell division rates in the root apical meristem, dependent on de novo local auxin biosynthesis and temperature-sensitive organization of the polar auxin transport system.
Article
Developmental Biology
Timon W. Matz, Yang Wang, Ritika Kulshreshtha, Arun Sampathkumar, Zoran Nikoloski
Summary: In this study, the authors used a network representation of live cell imaging data from shoot apical meristems (SAMs) in Arabidopsis thaliana to predict cell division events and their consequences at the tissue level. They found that a support vector machine classifier based on the SAM network properties is able to predict cell division events with high accuracy. Furthermore, the combination of topological and biological properties can improve the prediction accuracy of resulting changes in tissue topology triggered by cell division. The study also highlights the importance of microtubule-mediated cell-to-cell growth coordination in influencing tissue-level topology.
Article
Plant Sciences
Robert G. Orr, Fabienne Furt, Erin L. Warner, Erin M. Agar, Jennifer M. Garbarino, Sarah E. Cabral, Michelle L. Dubuke, Allison M. Butt, Mary Munson, Luis Vidali
Summary: The interaction between Rab-E and myosin XI is a conserved feature of polarised growth in plants, and it is essential for the normal plant growth. This interaction is specifically mediated by a certain residue in myosin XI, and it supports the spatial and temporal coupling of both proteins at sites of active exocytosis.
Article
Biology
Andreas Kolbeck, Peter Marhavy, Damien De Bellis, Baohai Li, Takehiro Kamiya, Toru Fujiwara, Lothar Kalmbach, Niko Geldner
Summary: Efficient formation of the plant Casparian Strip network relies on communication between neighboring cells, with the protease LOTR1 playing a crucial role. Impaired structure restriction leads to the formation of "half strips" at ectopic positions.
Article
Plant Sciences
Zhanchao Cheng, Changhong Mu, Xiangyu Li, Wenlong Cheng, Miaomiao Cai, Chongyang Wu, Jutang Jiang, Hui Fang, Yucong Bai, Huifang Zheng, Ruiman Geng, Junlei Xu, Yali Xie, Yuping Dou, Juan Li, Shaohua Mu, Jian Gao
Summary: In this study, a modified protocol for protoplast preparation was used to investigate the single-cell transcriptome of the root system in moso bamboo. Four cell types were identified, and new cell-type-specific marker genes for the basal root were defined. The developmental trajectories of the root cap, epidermis, and ground tissues were reconstructed, and key factors regulating cell fate determination were elucidated. Furthermore, the study revealed the divergence of root developmental pathways between moso bamboo and rice and identified key regulatory factors for cell differentiation.
HORTICULTURE RESEARCH
(2023)
Article
Developmental Biology
Tatsuaki Goh, Kaoru Sakamoto, Pengfei Wang, Saki Kozono, Koki Ueno, Shunsuke Miyashima, Koichi Toyokura, Hidehiro Fukaki, Byung-Ho Kang, Keiji Nakajima
Summary: The root cap is a tissue that guides root growth in plants. Its cells are constantly replaced through cell division and detachment. The transition of root cap cells from gravity-sensing cells to secretory cells involves rearrangement of organelles in the outer cell layer, which is partially regulated by autophagy. This developmentally regulated autophagy helps in the organized separation of living root cap cells, without causing immediate cell death.
Article
Plant Sciences
Yang Yang, Charles T. Anderson, Jiashu Cao
Summary: The study explores the functions of Arabidopsis PG45 in organ morphogenesis, revealing its significant role in leaf and flower structure, branch formation, and organ growth. Knockout and overexpression of PG45 result in altered leaf morphology and impaired cell proliferation, accompanied by changes in pectin metabolism and OG profiles. These findings underscore a previously unrecognized function for PGs in tissue polarity determination and cell proliferation regulation, suggesting the presence of OG-based signaling pathways in modulating plant development.
Review
Chemistry, Physical
Sajedeh Kerdegari, Paolo Canepa, Davide Odino, Reinier Oropesa-Nunez, Annalisa Relini, Ornella Cavalleri, Claudio Canale
Summary: This article reviews the advancements made in cell/tissue mechanics and adhesion using Atomic Force Microscopy (AFM)-based approaches. AFM offers high force sensitivity and a wide range of detectable forces, making it suitable for studying various biological issues. It enables accurate control of probe position during experiments, providing subcellular resolution mechanical maps of biological samples. The importance of mechanobiology in biotechnological and biomedical fields is recognized. The article discusses cell mechanosensing, the relationship between cell mechanical properties and pathological states (with a focus on cancer and neurodegenerative diseases), and the role of AFM in characterizing pathological mechanisms and developing new diagnostic tools using cell mechanics as tumor biomarkers. The article also highlights AFM's unique ability to study cell adhesion at the single-cell level and its relevance to understanding pathologies.
Article
Plant Sciences
Kazuya Ishikawa, Makoto Kobayashi, Miyako Kusano, Keiji Numata, Yutaka Kodama
Summary: By using the organelle glue technique, we manipulated organelle interactions and controlled the plant metabolome at the pathway level. This study demonstrates the potential for designing plants with desirable metabolic activities by artificially controlling organelle interactions through genetic modification.
PLANT CELL REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Federica Lupoli, Tommaso Vannocci, Giovanni Longo, Neri Niccolai, Annalisa Pastore
Article
Biochemical Research Methods
Simone Dinarelli, Marco Girasole, Giovanni Longo
BMC BIOINFORMATICS
(2018)
Article
Multidisciplinary Sciences
S. Dinarelli, G. Longo, G. Dietler, A. Francioso, L. Mosca, G. Pannitteri, G. Boumis, A. Bellelli, M. Girasole
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Francesco S. Ruggeri, Curtis Marcott, Simone Dinarelli, Giovanni Longo, Marco Girasole, Giovanni Dietler, Tuomas P. J. Knowles
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Microbiology
A. Mustazzolu, L. Venturelli, S. Dinarelli, K. Brown, R. A. Floto, G. Dietler, L. Fattorini, S. Kasas, M. Girasole, G. Longo
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Multidisciplinary Sciences
Tommaso Vannocci, Simone Dinarelli, Marco Girasole, Annalisa Pastore, Giovanni Longo
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Leonardo Venturelli, Anne-Celine Kohler, Petar Stupar, Maria Villalba, Aleksandar Kalauzi, Ksenija Radotic, Massimiliano Bertacchi, Simone Dinarelli, Marco Girasole, Milica Pesic, Jasna Bankovic, Maria E. Vela, Osvaldo Yantorno, Ronnie Willaert, Giovanni Dietler, Giovanni Longo, Sandor Kasas
JOURNAL OF MOLECULAR RECOGNITION
(2020)
Article
Biochemistry & Molecular Biology
Simone Dinarelli, Giovanni Longo, Stefano Cannata, Sergio Bernardini, Alessio Gomiero, Gianna Fabi, Marco Girasole
JOURNAL OF MOLECULAR RECOGNITION
(2020)
Review
Chemistry, Multidisciplinary
Roberto Scandurra, Anna Scotto D'Abusco, Giovanni Longo
Article
Chemistry, Multidisciplinary
Jiangtao Zhou, Leonardo Venturelli, Ludovic Keiser, Sergey K. Sekatskii, Francois Gallaire, Sandor Kasas, Giovanni Longo, Tuomas P. J. Knowles, Francesco S. Ruggeri, Giovanni Dietler
Summary: This study reveals a correlation between environmental stress and amyloid polymorphism, with high stress conditions favoring the formation of homogeneous amyloid fibrils.
Article
Microbiology
Maria Villalba, Leonardo Venturelli, Ronnie Willaert, Maria E. Vela, Osvaldo Yantorno, Giovanni Dietler, Giovanni Longo, Sandor Kasas
Summary: This study utilized AFM-based nanomotion detection technique to reveal the correlation between nanometer-scale bacterial motion pattern and the virulence state of bacteria, paving the way for novel rapid and label-free pathogenic microorganism detection assays.
Article
Biochemistry & Molecular Biology
Simone Dinarelli, Giovanni Longo, Antonio Francioso, Luciana Mosca, Marco Girasole
Summary: This paper investigates the relationship between aging and mechano-transduction in red blood cells and discovers that mechanical stimulation can accelerate the aging process. The behavior of cellular effectors and resources is evaluated in the presence of drugs and specific inhibitors. The findings provide a comprehensive perspective on cell-environment interactions and demonstrate the direct correlation between aging and mechano-transduction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Simone Dinarelli, Giovanni Longo, Stefka Germanova-Taneva, Svetla Todinova, Sashka Krumova, Marco Girasole
Summary: Favism is a unique disease caused by a genetic defect in the G6PD enzyme, resulting in a severe reduction in erythrocytes' reducing power and impaired ability to respond to oxidative stresses. Comparison of normal and favism cells during aging revealed distinct metabolic regulation in the G6DP-deficient cells, which leads to specific characteristics in cell properties. Favism cells exhibit higher resistance to external stresses and greater overall resilience during aging, which can be attributed to a special mechanism of metabolic regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Condensed Matter
Mariangela Lopreiato, Alessia Mariano, Rossana Cocchiola, Giovanni Longo, Pietro Dalla Vedova, Roberto Scandurra, Anna Scotto d'Abusco
Article
Chemistry, Multidisciplinary
Jiangtao Zhou, Francesco S. Ruggeri, Manuela R. Zimmermann, Georg Meisl, Giovanni Longo, Sergey K. Sekatskii, Tuomas P. J. Knowles, Giovanni Dietler