Article
Chemistry, Multidisciplinary
Philip M. Graybill, Aniket Jana, Rakesh K. Kapania, Amrinder S. Nain, Rafael Davalos
Summary: Post-electroporation cell force undergoes three distinct stages: initial rounding and loss of contractility, followed by a biphasic stage characterized by increased contractility and subsequent force relaxation, and finally cell elongation and recovery of contractility. Increasing voltages applied perpendicular to cell orientation lead to a significant drop in cell viability, indicating that contractile force is a more sensitive metric than cell shape to electroporation.
Editorial Material
Biochemical Research Methods
Xiaowei Zhuang
Summary: The recent development of genome-scale imaging allows for single-cell omics analysis in intact cells and tissues, enabling gene expression profiling, identification of cell types, and spatial mapping in complex tissues. The high spatial resolution of these approaches also allows for determining the spatial organizations of the genome and transcriptome inside cells, which are key regulatory mechanisms for gene expression.
Article
Multidisciplinary Sciences
Karren Dai Yang, Anastasiya Belyaeva, Saradha Venkatachalapathy, Karthik Damodaran, Abigail Katcoff, Adityanarayanan Radhakrishnan, G. Shivashankar, Caroline Uhler
Summary: The authors use autoencoders to learn a probabilistic coupling and map different data modalities to a shared latent space, presenting an approach for integrating vastly different modalities. The integration of imaging and transcriptomics is still an open challenge, but this method provides a framework for diverse applications in biomedical discovery.
NATURE COMMUNICATIONS
(2021)
Article
Physics, Applied
W. Milestone, Q. Hu, A. M. Loveless, A. L. Garner, R. P. Joshi
Summary: A distributed circuit approach was used to simulate the effects of voltage pulses on cell membrane potentials and poration dynamics, showing strong heating and power dissipation, increasing membrane temperature with successive pulses, and large temperature gradients.
JOURNAL OF APPLIED PHYSICS
(2022)
Review
Chemistry, Multidisciplinary
Bastien Duckert, Steven Vinkx, Dries Braeken, Maarten Fauvart
Summary: Recent advancements in gene editing and cell therapies have shown significant clinical successes, but the lack of a cost-effective manufacturing process hinders widespread adoption. Single-Cell Transfection Technologies (SCTTs) offer promising advantages such as higher efficiency and safety, but are currently underutilized in clinical settings.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Electrochemistry
Fei Guo, Kun Qian, Lin Zhang, Hao Deng, Xin Li, Jiong Zhou, Ji Wang
Summary: This study examines the effects of intense nanosecond pulsed electric fields on cell membrane electroporation, with a focus on the impact of normal electroporation and tangential dispersion on the process. The results show that normal electroporation reduces local pore density, leading to higher transmembrane voltage, while tangential dispersion increases pore density and accelerates electroporation. Considering both factors can expand the electroporated area and alter the spatial distribution of nanopores. The proposed model provides a more accurate representation of the spatiotemporal evolution of anisotropic parameters in nsPEF-induced electroporation, which can guide parameter selection in applications.
ELECTROCHIMICA ACTA
(2021)
Article
Agriculture, Dairy & Animal Science
Inna E. Pristyazhnyuk, Lyubov P. Malinovskaya, Pavel M. Borodin
Summary: We developed a simple method for establishing primary somatic cell cultures from the ovaries of great tits and the testes of ten Passerine species. The ovary-derived cell cultures showed high proliferative activity, while the testis-derived cell cultures had lower proliferation potential. However, both cell cultures provided sufficient material for genetic modification and cytogenetic studies.
Article
Chemistry, Multidisciplinary
Lujia Ding, Zheng Fang, Michael A. J. Moser, Wenjun Zhang, Bing Zhang
Summary: This study developed a mathematical model to investigate the evolution of pores during cell electroporation. The simulation results showed a sigmoid increase in pore area ratio (PAR) over time, indicating a cumulative effect of PAR. Additionally, the relationship between high-frequency irreversible electroporation (H-FIRE) parameters and the cumulative effect of PAR was established, allowing for effective design of cell ablation protocols.
APPLIED SCIENCES-BASEL
(2023)
Article
Engineering, Biomedical
Prithvijit Mukherjee, Chian -Yu Peng, Tammy McGuire, Jin Wook Hwang, Connor H. Puritz, Nibir Pathak, Cesar A. Patino, Rosemary Braun, John A. Kessler, Horacio D. Espinosa
Summary: We used single cell RNA sequencing to investigate the effects of BEP and LEP on gene expression in murine neural stem cells. Our results show that LEP does not lead to extensive cell death or activation of cell stress response pathways, making it suitable for long-term preservation of cell physiology. In addition, LEP enables better cell viability and integrity compared to BEP, making it a promising method for multi-day delivery protocols.
MATERIALS TODAY BIO
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Egemen Bilgin, Mehmet Cayoren, Sulayman Joof, Gokhan Cansiz, Tuba Yilmaz, Ibrahim Akduman
Summary: This study presents a prototype for microwave imaging of breast cancer, which aims to project the geometrical features of anomalies inside the breast to a single-slice image at the coronal plane for diagnosis.
Article
Pharmacology & Pharmacy
Tina Vindis, Anja Blazi, Diaa Khayyat, Tjasa Potocnik, Shaurya Sachdev, Lea Rems
Summary: In this study, a localized electroporation method based on commercially available cell culture inserts and porous substrates was designed to enhance gene electrotransfer efficiency while preserving cell viability. The device tested in mammalian cell lines showed similar transfection efficiencies to conventional bulk electroporation, but with lower voltage pulses and without the need for expensive electroporators.
Article
Chemistry, Multidisciplinary
Xiao Wei, Haoqi Li, Hao Li, Zewen Zuo, Fengqi Song, Xi Kang, Manzhou Zhu
Summary: Metal nanoclusters can be transformed into new nanoclusters with customized structures and enhanced performance using efficient strategies. However, tracking the nanocluster transformations has been challenging. In this study, a slice visualization approach was used to monitor the transformation of Au1Ag24(SR)(18) to Au1Ag30(SR)(20) nanoclusters. The mechanism of nanocluster structure growth was mapped and intermediate products were observed with atomic precision. This approach not only provides a platform for studying structure-property correlations but also offers a means to obtain clear information on nanocluster structure evolution.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biophysics
T. (Tim) Bognar, J. S. (Jurjen) Kingma, E. H. (Erin) Smeijsters, K. C. M. (Kim) van der Elst, C. T. M. (Klaartje) de Kanter, C. A. (Caroline) Lindemans, A. C. G. (Toine) Egberts, I. H. (Imke) Bartelink, A. (Arief) Lalmohamed
Summary: This observational study assessed the impact of therapeutic drug monitoring on busulfan exposure in pediatric patients undergoing hematopoietic cell transplantation. The results showed no significant difference in achieving therapeutic busulfan exposure between the two monitoring regimens, suggesting that day 1-guided dosing may be sufficient with subsequent monitoring if needed.
BONE MARROW TRANSPLANTATION
(2023)
Article
Immunology
Chuan Yan, Qiqi Yang, Songfa Zhang, David G. Millar, Eric J. Alpert, Daniel Do, Alexandra Veloso, Dalton C. Brunson, Benjamin J. Drapkin, Marcello Stanzione, Irene Scarfo, John C. Moore, Sowmya Iyer, Qian Qin, Yun Wei, Karin M. McCarthy, John F. Rawls, Nick J. Dyson, Mark Cobbold, Marcela Maus, David M. Langenau
Summary: This study utilized optically clear immunocompromised zebrafish models engrafted with fluorescent-labeled human cancers and various T cell immunotherapies to visualize T cell immune responses at single-cell resolution in real time. Differences in kinetics of T cell infiltration, tumor cell engagement, and killing were identified among the immunotherapies, leading to early endpoint analysis for predicting therapy responses. EGFR-targeted immunotherapies were established as a powerful approach to eradicate rhabdomyosarcoma muscle cancers, suggesting the potential of assessing a broader range of T cell immunotherapies in this disease.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Yue Wu, Afu Fu, Gilad Yossifon
Summary: In this study, a metallodielectric hybrid micromotor was used to perform localized electroporation and gene transfection of mammalian cells, showing high reversible electroporation yield and precise targeting of cells. The micromotor was able to enhance gene introduction into difficult-to-transfect cells and demonstrated successful gene expression post transfection, which is significant for molecular biology research.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)