Journal
MICROFLUIDICS AND NANOFLUIDICS
Volume 10, Issue 3, Pages 593-605Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10404-010-0692-2
Keywords
Microfluidics; ELISA; Lab-on-a-chip; Magnetic beads; Capillary force
Funding
- National Science Foundation [NSF-OISE-0530203]
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We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are pre-encapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (mu IPELISA). mu IPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use mu IPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng mu l(-1) of digoxigenin-labeled amplicon, mu IPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct mu IPELISA in two parallel microchannels.
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