Article
Green & Sustainable Science & Technology
Ahmed A. Ayash, Harith H. Al-Moameri, Ali Abed Salman, Arnold A. Lubguban, Roberto M. Malaluan
Summary: This study presents a novel microfluidic approach for complete separation of blood cells. The approach utilizes a polydimethylsiloxane serpentine microchannel with a series of electrodes, providing two separation zones. The design exploits the unique channel configuration and the inherent differences in dielectric properties of the three blood cell types to achieve size-based sorting. Platelets are separated in the first zone due to their larger dielectrophoretic force compared to red and white blood cells. The model developed in this work demonstrates the feasibility of the proposed approach, showing complete separation of blood content across different flow rates and AC frequencies.
Article
Engineering, Environmental
Alexander Kovalev, Anna Yagodnitsyna, Artur Bilsky
Summary: The study focuses on the hydrodynamic features of liquid-liquid flows in serpentine microchannels to control mixing and mass transfer. Different curvatures of the microchannels lead to changes in plug acceleration/deceleration, plug length, and flow patterns in both oil slugs and aqueous plugs. Optimal conditions for efficient mixing and mass transfer in the plug flow regime are determined based on the research findings.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Analytical
Lei Zhao, Mengqi Gao, Yanbing Niu, Jianchun Wang, Shaofei Shen
Summary: A novel microchannel structure was developed for flow-rate and particle-size insensitive inertial focusing; efficient focusing of particles and tumor cells at different flow rates was achieved through enhanced secondary flow; providing important reference for the development of portable inertial microfluidic devices.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Maliha Saleem Bakhshi, Mohsin Rizwan, Ghulam Jilany Khan, Hong Duan, Kefeng Zhai
Summary: In this study, a novel two-stage, label-free, rapid, and continuous CTC separation device based on hydrodynamic inertial focusing and dielectrophoretic separation is proposed. The device can effectively separate CTCs from other blood cells, yielding viable CTCs with high efficiency.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Yong Qin, Liang-Liang Fan, Liang Zhao
Summary: A new method using a reverse external force was proposed to efficiently focus aerosol particles in a microchannel. A numerical model of particle movement in the microchannel was established, and simulations were conducted to study the gas-particle two-phase flow under the effect of the reverse external force. The results showed that a suitable reverse external force could significantly enhance the Saffman lift force on the aerosol particle, resulting in efficient particle focusing in the microchannel.
Article
Engineering, Chemical
Haotian Cha, Hoseyn A. Amiri, Sima Moshafi, Ali Karimi, Ali Nikkhah, Xiangxun Chen, Hang T. Ta, Nam-Trung Nguyen, Jun Zhang
Summary: Inertial microfluidics is a technique that uses the finite inertia of fluid at high flow speed to manipulate and separate microparticles. Embedding periodic micro-obstacles into curvilinear channels has been found to be an effective strategy to improve inertial focusing and separation. This study systematically investigated the influence of micro-obstacles on inertial focusing and developed a high-resolution microfluidic device for particle and cell separation. The results showed that concave obstacles were more effective in tuning particle inertial focusing and separation compared to convex obstacles, and the square concave obstacle channel offered the highest separation resolution. The developed microfluidic device showed high-efficiency separation of polystyrene beads and U87MG cancer cells from blood.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Chemistry, Analytical
Uihwan Kim, Byeolnim Oh, Jiyeon Ahn, Sangwook Lee, Younghak Cho
Summary: In this study, an integrated microfluidic device was proposed for non-contact, label-free separation of particles and cells using a combination of inertial microfluidics and acoustophoresis. The device demonstrated excellent separation performance and high recovery ratio, making it a potential alternative to current cell separation methods.
Article
Chemistry, Multidisciplinary
Yongxin Song, Xiaoshi Han, Deyu Li, Qinxin Liu, Dongqing Li
Summary: A novel microfluidic method was proposed to count the number of particles while they are separated by localized DC-dielectrophoresis force. The separation and counting of particles of different sizes, including polystyrene particles, were successfully demonstrated with a resolution of 1 μm. This method allows for simultaneous separation and in situ counting of particles, showing potential for various applications in particle analysis.
Article
Biochemical Research Methods
Atakan Atay, Ali Beskok, Barbaros Cetin
Summary: The DC-electrokinetic behavior of colloidal cylinders near a conducting wall is investigated, taking into account the boundary effects and the contribution of different forces. By incorporating the electrokinetic and colloidal forces into a computational model and applying the electrokinetic slip-velocity and thin electrical double layer assumption, the position, velocity, electric field, and flow field of the particles are analyzed.
Article
Mechanics
Giridar Vishwanathan, Gabriel Juarez
Summary: Oscillatory flows have the potential to overcome limitations of using steady flows for inertial focusing due to low particle Reynolds numbers. Unsteady inertial forces on inertial focusing have not been thoroughly examined. Experimental and theoretical studies show that oscillatory flows are remarkably efficient for inertial focusing, even at low particle Reynolds numbers.
JOURNAL OF FLUID MECHANICS
(2021)
Article
Engineering, Electrical & Electronic
Liang Huang, Guiqiang Wang, Guoqin Zhan, Pei Pei
Summary: The study developed a microchip with 3D electrodes and wavy microchannels for cell focusing and separation using the synergistic effect of DEP and fluidic forces. Experimental results showed successful separation of A549 and HeLa cells within a specific frequency range, with improved separation efficiency.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2021)
Article
Computer Science, Theory & Methods
Eugen Chiriac, Marioara Avram, Corneliu Balan
Summary: The paper discusses the method of using dielectrophoresis to separate cancer cells from blood cells, simulations conducted using COM-SOL Multiphysics can be extended to testing multiple CTC lines at various flow velocities and electric field frequencies.
ROMANIAN JOURNAL OF INFORMATION SCIENCE AND TECHNOLOGY
(2021)
Article
Thermodynamics
Yi Peng, Zhibin Li, Shaobo Li, Bin Cao, Xuan Wu, Xianfeng Zhao
Summary: The paper presents a zigzag-serpentine microchannel heat sink (ZSMHS) designed to study heat transfer and hydraulic performance through experiments. Different angles of the zigzag microchannel were investigated, with 30 degrees providing the lowest pressure drop, friction coefficient, and thermal resistance. The serpentine microchannel improved the hydraulic performance of the ZSMHS.
INTERNATIONAL JOURNAL OF THERMAL SCIENCES
(2021)
Article
Chemistry, Analytical
Qiang Zhao, Yunjiao Wang, Bangyong Sun, Deqiang Wang, Gang Li
Summary: This research presents a dielectrophoretic manipulation technique based on nanogap electrodes, which allows for the capture, enrichment, and sorting of nanometer-sized objects in liquids. The technique shows potential applications in micro/nanofluidics and provides an alternative method for the non-invasive manipulation and characterization of nanoparticles such as DNA, proteins, and viruses.
Article
Biochemical Research Methods
Joseph Bentor, Amirreza Malekanfard, Mahmud Kamal Raihan, Sen Wu, Xinxiang Pan, Yongxin Song, Xiangchun Xuan
Summary: Experimental study shows that rheological properties of fluids significantly impact the focusing and trapping effects in iDEP microdevices. Strongly viscoelastic polyacrylamide solution demonstrates the best performance, while xanthan gum solution is not suitable for iDEP focusing.
Article
Chemistry, Analytical
Song-Yu Lu, Amirreza Malekanfard, Shayesteh Beladi-Behbahani, Wuzhou Zu, Akshay Kale, Tzuen-Rong Tzeng, Yao-Nan Wang, Xiangchun Xuan
Article
Biophysics
Eric S. McLamore, Evangelyn Alocilja, Carmen Gomes, Sundaram Gunasekaran, Daniel Jenkins, Shoumen P. A. Datta, Yanbin Li, Yu (Jessie) Mao, Sam R. Nugen, Jose Reyes-De-Corcuera, Paul Takhistov, Olga Tsyusko, Jarad P. Cochran, Tzuen-Rong (Jeremy) Tzeng, Jeong-Yeol Yoon, Chenxu Yu, Anhong Zhou
Summary: The biosensor research in North America faces challenges and opportunities, calling for platform approaches including tools supporting interoperability, open-source analytics tools, data algorithms, and sensor designs inspired by usage. The analysis focused on mobile devices and phone-based biosensors to enhance decision support. Results show a concentration on fundamental platform development and material characterization in biosensor research, with optical transduction being predominant in food and environmental applications, while electrochemical transduction is common in agricultural applications, with limited use of mobile platforms.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yueyuan Zhang, Paul L. Dawson, Timothy W. Hanks, Julie K. Northcutt, Tzuen-Rong Tzeng, William T. Pennington
Summary: Membrane filters coated with polymerized PCDA were used for rapid bacterial detection, with successful color changes in response to Salmonella Typhimurium and Escherichia coli. The time required for color change was determined visually and validated with a linear regression model. The method accurately estimated Salmonella Typhimurium populations within a range of 8 to 3 log CFU ml(-1) in 1.5-7.5 hours.
Article
Chemistry, Analytical
Amirreza Malekanfard, Shayesteh Beladi-Behbahani, Tzuen-Rong Tzeng, Hui Zhao, Xiangchun Xuan
Summary: This study introduces a technique for particle/cell focusing using low-frequency AC electric fields, demonstrating its effectiveness compared to DC iDEP focusing with a second-order power relationship for improved efficiency. The AC iDEP focusing method eliminates the need for large electric fields and small constrictions, offering better performance benefits.
ANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Tzuen-Rong J. Tzeng, Tzu-Yu Liu, Chiao-Wei Lin, Pei-En Chang, Pei-Xin Liao, Wen-Yuan Yang, Chih-Yuan Cheng, Pei-Chun Liao, Wen-Dee Chiang, Shih-Torng Ding, Yuan-Yu Lin
Summary: The study shows that Hydrastis canadensis can improve egg albumen height and modulate the cecum digesta microbiota composition of laying hens, providing additional health benefits for the chickens.
Article
Chemistry, Multidisciplinary
Yash S. Raval, Anna Samstag, Cedric Taylor, Guohui Huang, Olin Thompson Mefford, Tzuen-Rong Jeremy Tzeng
Summary: PEO-IONPs exhibited cytotoxicity to normal human colon cells, while GM3-IONPs were relatively safe. PEO-IONPs affected cell viability, ATP synthase activity, and membrane integrity, while GM3-IONPs caused changes in cell morphology without membrane damage.
Article
Materials Science, Multidisciplinary
Sarah M. Helms, Liam O'Neill, Shayesteh Beladi Behbahani, Jeremy Tzeng, Kyle Jeray, Marian S. Kennedy, Andrew W. Cross, Stephanie L. Tanner, John D. DesJardins
Summary: This study examined the efficacy of co-deposited vancomycin/chitosan coatings on metallic implants using a plasma process in a bacterially challenged rabbit model. The thin chitosan and vancomycin coating showed antimicrobial properties, prevented bacterial adhesion, and inhibited biofilm formation. Both roughening and partial implantation of alumina particles on titanium alloy implants after blasting did not affect the short-term antimicrobial effects of the chitosan/vancomycin coating.
Article
Toxicology
Carlos Angele-Martinez, Fathima S. Ameer, Yash S. Raval, Guohui Huang, Tzuen-Rong J. Tzeng, Jeffrey N. Anker, Julia L. Brumaghim
Summary: Polyphenolic antioxidants have varying effects on preventing DNA damage and cytotoxicity induced by (CuO)-Cu-NP and H2O2, with some being protective, some having no effect, and some even increasing DNA damage. Additionally, polyphenols can modulate levels of reactive oxygen species (ROS) generated, and protect DNA under oxidative stress conditions.
TOXICOLOGY IN VITRO
(2022)
Review
Biochemical Research Methods
Chenxu Yu, Paul Takhistov, Evangelyn Alocilja, Jose Reyes de Corcuera, Margaret W. Frey, Carmen L. Gomes, Yu J. Mao, Eric S. McLamore, Mengshi Lin, Olga V. Tsyusko, Tzuen-Rong J. Tzeng, Jeong-Yeol Yoon, Anhong Zhou
Summary: This review discusses the presence and impact of microplastics and nanoplastics throughout the agricultural and food supply chain, focusing on the methods for their detection and characterization. The lack of standardized and reliable detection methods remains a challenge, and the development of rapid and high-throughput field-deployable methods is a high priority.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Kendall Gassman, Sarah G. Hill, Nathan D. Smith, Marian S. Kennedy, Tzuen-Rong Tzeng, Shayesteh Beladi Behbahani, Sarah M. Helms, Liam O'Neill, John D. DesJardins
Summary: Orthopaedic hardware infections are a significant burden and chitosan surface modifications can reduce infection rates. Surface roughness and chitosan concentration have a significant impact on biofilm growth.
Review
Microbiology
Shayesteh Beladi Behbahani, Sachindra D. Kiridena, Uthpala N. Wijayaratna, Cedric Taylor, Jeffrey N. Anker, Tzuen-Rong Jeremy Tzeng
Summary: The advent of implanted medical devices has improved the quality of life and increased longevity, but infection remains a significant risk. Measuring the pH in biofilms is crucial for understanding their biochemistry and offers potential routes for detecting and treating latent infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Apeksha C. Rajamanthrilage, Erin Levon, Unaiza Uzair, Cedric Taylor, Tzuen-Rong Tzeng, Jeffrey N. Anker
Summary: Researchers have described a technique called XELCI that allows noninvasive imaging of chemical concentrations near the surface of implantable medical devices to monitor infections. By coating the devices with chemically reporting surfaces and scanning them with a focused X-ray beam, researchers can measure the spectral ratio of light passing through the tissue and generate an image to study the distribution of chemical concentrations.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Chemistry, Analytical
Kelli M. McCourt, Jarad Cochran, Sabah M. Abdelbasir, Elizabeth R. Carraway, Tzuen-Rong J. Tzeng, Olga V. Tsyusko, Diana C. Vanegas
Summary: This article discusses the toxicity of nanomaterials used in biosensor development and highlights five factors that should be considered to manage toxicity. It emphasizes the importance of a precautionary "One Health" approach and circular use to mitigate the risks of uncontrolled nanomaterial release.
Article
Chemistry, Multidisciplinary
Sachindra D. D. Kiridena, Uthpala N. N. Wijayaratna, Erin Levon, Phillip Moschella, Ronald G. G. Pirrallo, Tzuen-Rong J. Tzeng, Jeffrey N. N. Anker
Summary: A hydrogel-based peritoneal fluid pH sensor has been developed to detect and monitor peritoneal infections in patients undergoing peritoneal dialysis. The sensor, attached to a catheter and implanted in a rat peritoneum, showed a rapid pH drop during infection. This is the first report of an in vivo chemically responsive hydrogel sensor.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yueyuan Zhang, Paul L. Dawson, Tzuen-Rong Tzeng, Timothy W. Hanks, Julie K. Northcutt, William T. Pennington
RESULTS IN CHEMISTRY
(2020)
