Journal
MICROCHIMICA ACTA
Volume 182, Issue 1-2, Pages 409-417Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-014-1352-0
Keywords
Copper sulfide nanosheet; Au nanoparticles; 17 beta-estradiol; Glucose oxidase; Signal amplification
Categories
Funding
- National Natural Science Foundation of China [U1304214]
- State Key Laboratory of Chemo/biosensing and Chemometrics [2013013]
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We have developed an electrochemical method for the determination of 17 beta-estradiol. A glassy carbon electrode was modified with a composite made from copper sulfide nanosheets, gold nanoparticles, and glucose oxidase. The copper sulfide nanosheet was prepared by a single-step hydrothermal process, and its properties were characterized by X-ray powder diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy and transmission electron microscopy. Finally, an estradiol-specific aptamer was assembled on the electrode. The copper sulfide nanosheet on the electrode surface acts as a relatively good electrical conductor. Glucose oxidase acts as an indicator, and the dual modification of glucose oxidase and gold nanoparticles for signal amplification. The determination of 17 beta-estradiol was performed by differential pulse voltammetry of glucose oxidase because the signal measured at typically -0.43 V depends on the concentration of 17 beta-estradiol because addition of 17 beta-estradiol at electrode hinders electron transfer. A linear relationship exists between the peak current and the logarithm of concentration of 17 beta-estradiol in the 0.5 pM to 5 nM range, with a 60 f. detection limit (at 3 sigma/S). The method displays good selectivity over bisphenol A, 1-aminoanthraquinone and naphthalene even if present in 100-fold concentrations.
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