Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Biochemistry & Molecular Biology
Yuqing Xia, Xin Lei, Xiaochen Ma, Shizheng Wang, Zifu Yang, Yifan Wu, Xiaojun Ren
Summary: In this paper, we developed a dual-signal isothermal amplification method based on rolling circle amplification (RCA) coupled with DNAzyme (RCA-DNAzyme) for sensitive detection of low-abundance exosome RNA. By designing padlock probes and relying on the ligation reaction, the specific recognition and precise targeting of target exRNA were achieved. The combination of RCA and DNAzyme achieved a twice-as-large isothermal amplification of the signal compared to RCA alone. The technology was successfully proven to be effective for the quantitative detection of miR-21 by spike recovery, providing a new research approach for the accurate detection of low-abundance exRNA and the exploration of unknown exRNA functions.
Article
Chemistry, Analytical
Shuya He, Yan Zhou, Yaoqi Xie, Kaijiong Zhang, Qiao He, Gang Yin, Haimin Zou, Qiongying Hu, Shilu Zhang, Hongfei He, Dongsheng Wang
Summary: Due to the complexity of detecting nucleic acids in clinical samples and the low abundance of the target, false positives caused by nonspecific amplification are common. This study presents a specific signal extract-to-output isothermal detection system (SSEI) that can detect as low as 10 copies of target DNA per reaction, overcoming the limitations of other nucleic acid isothermal amplification techniques.
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Article
Chemistry, Analytical
Haobo Sun, Lisheng Qian, Jinming Kong, Xueji Zhang
Summary: A novel electrochemical biosensor platform with triple helix structure switch and double signal amplification strategy was proposed for nucleic acid detection, achieving ultra-sensitivity and high selectivity. The biosensor showed a detection limit as low as 1.954 aM and a linear range of 7 orders of magnitude. Additionally, it demonstrated excellent anti-interference ability when analyzing DNA in serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Analytical
Qiuyuan Lin, Guobin Han, Xueen Fang, Hui Chen, Wenhao Weng, Jilie Kong
Summary: The study presents a general and programmable diagnostic strategy for miRNA analysis. By combining exponential isothermal amplification and programmable TtAgo cleavage, this method enables rapid and sensitive multiplex detection of miRNAs.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiuen Cao, Chuanpin Chen, Qubo Zhu
Summary: This review summarizes the applications of functional nucleic acids and isothermal amplification techniques in biosensing, highlighting their important contributions to the field. These technologies have great potential to enhance the performance of biosensors.
Article
Chemistry, Analytical
Nan Zong, Ying Gao, Yong Chen, Xueqing Luo, Xingyu Jiang
Summary: A centrifugal microfluidic platform was developed for combined pretreatment and molecular diagnosis. Multiple signal enhancement systems were used to avoid false-negative results. The platform successfully detected and genotyped hepatitis B virus from whole blood. Compared to traditional diagnostic processes, the platform significantly reduced time consumption and the need for professional labor.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Jeong Moon, Jayeon Song, Hyowon Jang, Hyunju Kang, Yong-Min Huh, Hye Young Son, Hyun Wook Rho, Mirae Park, Chandana S. Talwar, Kwang-Hyun Park, Euijeon Woo, Jaewoo Lim, Eun-Kyung Lim, Juyeon Jung, Yongwon Jung, Hyun Gyu Park, Taejoon Kang
Summary: In this study, a ligation-free DNA extension method for nucleic acid amplification was developed. This method allows for high sensitivity detection of target RNA and shows potential applications in cancer companion diagnostics and other gene detection platforms.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Yuanyuan Zhu, Jian Wu, Qingli Zhou
Summary: Functional DNA (fDNA) refers to DNA molecules that have specific binding ability or catalytic function, with advantages of simple synthesis, low cost, low toxicity, high chemical stability, recognition specificity, and biocompatibility. However, the limited sensitivity of fDNA sensors to trace targets is a major challenge, especially when there is low affinity between fDNA and the targets. Various nucleic acid signal amplification strategies (NASAS) have been explored to improve the limit of detection of fDNA. This review summarizes four NASAS (hybridization chain reaction, entropy-driven catalysis, rolling circle amplification, CRISPR/Cas system) and their corresponding design principles, as well as the principle and application of fDNA sensors integrated with signal amplification strategies for the detection of non-nucleic acid targets. The main challenges and application prospects of NASAS-integrated fDNA biosensing system are also discussed.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Chao Zhang, Tarun Belwal, Zisheng Luo, Bin Su, Xingyu Lin
Summary: This article comprehensively discusses the application of different nanomaterials in isothermal nucleic acid amplification, including the roles of 0D, 1D, 2D, and 3D nanomaterials, as well as recent progress in the field. Nanomaterials are commonly used for reaction enhancement, signal generation/amplification, or surface loading. Additionally, 3D nanomaterials can serve as isolated chambers for digital nucleic acid amplification and DNA sequencing tools, while challenges and recommendations are proposed for future applications.
Article
Chemistry, Analytical
Yin Zhao, Dongjuan Chen, Zhichen Xu, Tao Li, Jiang Zhu, Rui Hu, Guoyong Xu, Ying Li, Yunhuang Yang, Maili Liu
Summary: Fast and simplified detection of HPV using a microfluidic dual-droplet device (M-D3) was developed. The device combined the CRISPR-Cas12a system and multiplexed recombinase polymerase amplification (RPA) assay to simultaneously detect HPV16 and HPV18. The M-D3 platform demonstrated high sensitivity and specificity in detecting HPV DNA, allowing on-chip detection within 30 minutes. The platform was validated using clinical patient samples with HPV infection risk, showing promising results in terms of sensitivity and specificity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yong Chen, Xiaoling Xu, Jiachun Wang, Yibin Zhang, Wentao Zeng, Yizhen Liu, Xueji Zhang
Summary: A photoactivated CRISPR/Cas12a strategy was proposed in this study to achieve high-sensitivity nucleic acid detection in one pot, avoiding amplicon contamination and lowering the threshold for molecular diagnostics in POC.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qian Zhou, Youxiu Lin, Mingdi Xu, Zhuangqiang Gao, Huanghao Yang, Dianping Tang
ANALYTICAL CHEMISTRY
(2016)
Article
Biophysics
Mingdi Xu, Zhuangqiang Gao, Qian Zhou, Youxiu Lin, Minghua Lu, Dianping Tang
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Biophysics
Mingdi Xu, Zhuangqiang Gao, Qiaohua Wei, Guonan Chen, Dianping Tang
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Chemistry, Analytical
Zhuangqiang Gao, Yuping Lin, Yu He, Dianping Tang
Article
Chemistry, Multidisciplinary
Zhuangqiang Gao, Haihang Ye, Dianyong Tang, Jing Tao, Sanaz Habibi, Adrienne Minerick, Dianping Tang, Xiaohu Xia
Article
Chemistry, Multidisciplinary
Zhuangqiang Gao, Haihang Ye, Qingxiao Wang, Moon J. Kim, Dianyong Tang, Zheng Xi, Zhiyuan Wei, Shikuan Shao, Xiaohu Xia
Article
Chemistry, Multidisciplinary
Zheng Xi, Xun Cheng, Zhuangqiang Gao, Mengjing Wang, Tong Cai, Michelle Muzzio, Edwin Davidson, Ou Chen, Yeonwoong Jung, Shouheng Sun, Ye Xu, Xiaohu Xia
Article
Chemistry, Multidisciplinary
Zhuangqiang Gao, Shikuan Shao, Weiwei Gao, Dianyong Tang, Dianping Tang, Shengli Zou, Moon J. Kim, Xiaohu Xia
Summary: The study introduces an effective method for designing morphology-stable nanoparticles with tunable plasmonic properties by controlling internal structure; Plasmonic properties of the nanoparticles can be conveniently adjusted by varying the material deposited on the inner surfaces without changing the overall morphology; The nanoparticles show potential applications in colorimetric sensing with low detection limits.
Article
Chemistry, Multidisciplinary
Chuanyu Wang, Chung-Hui Huang, Zhuangqiang Gao, Jialiang Shen, Jiacheng He, Alana MacLachlan, Chao Ma, Ya Chang, Wen Yang, Yuxin Cai, Yang Lou, Siyuan Dai, Weiqiang Chen, Feng Li, Pengyu Chen
Summary: Tumor-derived exosomes are crucial in cancer development, and quantitative analysis of exosomes can provide valuable predictive biomarkers. The role of exosomal PD-L1 in immune checkpoint therapy suggests the potential clinical application of exosomes.
Article
Chemistry, Multidisciplinary
Zhuangqiang Gao, Yujing Song, Te Yi Hsiao, Jiacheng He, Chuanyu Wang, Jialiang Shen, Alana MacLachlan, Siyuan Dai, Benjamin H. Singer, Katsuo Kurabayashi, Pengyu Chen
Summary: A machine-learning-assisted microfluidic nanoplasmonic digital immunoassay was developed for rapid and accurate monitoring of cytokine storm in COVID-19 patients, enabling simultaneous detection of multiple cytokines with wide working ranges and ultralow detection limits. The immunoassay demonstrated high throughput and efficiency, and the image processing method enhanced by CNN significantly reduced processing time while maintaining high statistical accuracy, showing promise as an intelligent immunoassay for next-generation immune monitoring.
Article
Biophysics
Yeyu Wang, Qiqi Yang, Zhuangqiang Gao, Haifeng Dong
Summary: MicroRNAs (miRNAs) are small, single-stranded, and non-coding RNA molecules that regulate gene expression and are closely related to major human diseases. Developing accurate miRNA detection strategies is crucial for early diagnosis and treatment. Functional nucleic acid nanostructures, including RNA aptamers and DNAzymes selected by SELEX, have advantages in miRNA detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Materials Science, Multidisciplinary
Yang Wang, Zhuangqiang Gao, Bo Liu, Xiaohu Xia
JOURNAL OF MATERIALS CHEMISTRY C
(2019)
Article
Chemistry, Multidisciplinary
Jiuxing Li, Zhuangqiang Gao, Haihang Ye, Shulin Wan, Meghan Pierce, Dianping Tang, Xiaohu Xia
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Analytical
Zhuangqiang Gao, Grace G. Liu, Haihang Ye, Robert Rauschendorfer, Dianping Tang, Xiaohu Xia
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Zhuangqiang Gao, Shuzhen Lv, Mingdi Xu, Dianping Tang