Journal
MICROCHIMICA ACTA
Volume 181, Issue 3-4, Pages 295-301Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-013-1105-5
Keywords
Magnetoferritin; Magnetic nanoparticles; Peroxidase-like activity; Hydrogen peroxide; Oxidative stress
Categories
Funding
- Slovak Scientific Grant Agency VEGA [2/0045/13]
- METAMYLC [APVV 0171-10]
- European Structural Funds [26110230061, 26220120021]
- Ministry of Education of the Czech Republic [LD13021]
Ask authors/readers for more resources
Magnetoferritin is a spherical biomacromolecule with a diameter of about 12 nm. It consists of a protein shell composed of apoferritin that is surrounding magnetic nanoparticles of magnetite (Fe3O4) or maghemite (gamma-Fe2O3). Magnetoferritins with various iron content (loading factor) were synthetically prepared and their peroxidase-like activities studied via the oxidation of the chromogenic substrate N,N-diethyl-p-phenylenediamine sulfate by hydrogen peroxide to give a purple product with an absorption maximum at 551 nm. Magnetoferritin with higher loading factor exhibits a higher peroxidase-like activity. The catalytic activity was successfully applied to the determination of hydrogen peroxide in the 5.8 to 88.2 mM concentration range.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available