Journal
MICROCHIMICA ACTA
Volume 173, Issue 3-4, Pages 347-352Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-011-0558-7
Keywords
DNA-hybridization biosensor; DNA damage; Cd(II) ion; Co(phen)(3)(3+); Methylene blue
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Funding
- National Natural Science Foundation of China [20775002]
- Program for Innovative Research Team in Anhui Normal University
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A DNA biosensor was constructed by immobilizing a 20-mer oligonucleotide probe and hybridizing it with its complementary oligomer on the surface of a glassy carbon electrode modified with gold nanoparticles. The properties of the biosensor and its capability of recognizing its complementary sequence were studied by electrochemical impedance spectroscopy. The oxidative stress caused by cadmium ions can be monitored by differential pulse voltammetry using the cobalt(III)tris(1,10-phenanthroline) complex and methylene blue as electrochemical indicators. The biosensor is capable of indicating damage caused by Cd(II) ions in pH 6.0 solution. The results showed that the biosensor can be used for rapid screening for DNA damage.
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