Journal
MICROCHIMICA ACTA
Volume 174, Issue 1-2, Pages 123-130Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-011-0592-5
Keywords
Amperometric detection; Microchip capillary electrophoresis; Carbon paste electrode; 3-aminophenol; Drug impurity test
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Funding
- National Natural Science Foundation of China [20727006]
- Guangdong Provincial Science and Technology Project [2008A030102009]
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We report on a simple amperometric detector for use in microchip capillary electrophoresis. A disposable syringe serves as the electrode holder that is fixed at the outlet of the separation channel. A carbon paste electrode is used to detect dopamine (DA) and catechol (CA) after electrophoretic separation. The two model analytes were well separated within 60 s. The response is linear in the concentration range from 4 to 500 mu M, and the detection limit is 1.2 mu M for DA (S/N = 3:1). The relative standard deviations of the inter-run and inter-electrode peak currents, respectively, are 2.8% and 5.7% for DA, and 3.9% and 6.5% for CA. Favorable column efficiency (expressed by the theoretical plate number which is 5.3 x 104 m(-1) for DA) is achieved. The method was successfully applied to the separation and detection of 3-aminophenol (3-AP) in an injection powder containing sodium 4-aminosalicylate. The detection limit of 3-AP is as low as 1.7 mu M, which meets the demand of the impurity test. The facile assembly allows convenient replacement of working electrodes and improves the longevity of the microanalytical system.
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