Journal
MICROCHIMICA ACTA
Volume 167, Issue 3-4, Pages 179-185Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-009-0229-0
Keywords
Capillary zone electrophoresis; Beta-cyclodextrin; Amperometric detection; Flavonoids; Phenolic acid; Chinese herbal tea
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Funding
- Science and Technology Commission of Shanghai [08142201400]
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Kaempferol, apigenin, rutin, quercetin, luteolin and ferulic acid are separated and detected in Chinese herbal tea using capillary zone electrophoresis coupled to amperometric detection. The phosphate running buffer also contains -cyclodextrin (-CD), which assists in separation and gives excellent separations within 20 min and detection limits as low as 10 ng mL(-1) (S/N = 3). The effects of working electrode potential, pH and concentration of running buffer, concentration of -CD, separation voltage and injection time were investigated. The method was applied to analyze tea samples with recoveries in the range of 90.0 to 107.0%. The method offers high separation efficiency, short analysis time, small sample consumption, and good repeatability.
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