Journal
MICROCHEMICAL JOURNAL
Volume 96, Issue 2, Pages 301-307Publisher
ELSEVIER
DOI: 10.1016/j.microc.2010.05.004
Keywords
Laccase; Redox mediator; Voltammetry; Biosensor; Wine; Catecholamines
Categories
Funding
- Sapienza University of Rome
- European Commission [017350 (BioMedNano)]
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Bioelectrochemical properties of Trametes versicolor Laccase (TvL) and Trametes hirsuta Laccase (ThL) immobilized by using polyazetidine prepolymer (PAP) onto multi-walled carbon nanotubes (MWCNTs) screen printed electrode (SPE) surface, have been studied with several redox mediators by cyclic voltammetry (CV). The efficient entrapment of lactase in the PAP layer was confirmed by determination of both kinetic parameters (maximum current and Michaelis-Menten apparent constant) and analytical performances by chronoamperometry. The Laccase-modified MWCNTs electrode provides an effective biosensor for determination of polyphenols and catecholamines in real matrices; performances of the considered biosensors for real samples analysis are also compared and discussed. (C) 2010 Elsevier B.V. All rights reserved.
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