Journal
MICROCHEMICAL JOURNAL
Volume 93, Issue 1, Pages 12-16Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2009.03.008
Keywords
Mercury speciation; Monomethylmercury; Human cell lines; Genotoxicity
Categories
Funding
- Agencia Espanola de Cooperacion Internacional (AECI) [A/6556/06, BOE 10 enero 2007]
Ask authors/readers for more resources
A method for mercury speciation analysis on cell lines of the human central nervous system has been developed in order to study the intracellular mercury uptake and the distribution of mercury species. Glioblastoma (U373) and neuroblastoma (B103) human cell lines were exposed to different levels of monomethylmercury (MMHg). Mercury species were simultaneously extracted with tetramethylammonium hydroxide (TMAH) using a closed-vessel microwave system. The analysis was performed by ethylation and injection into a CGC-pyro-AFS system. An excellent procedural detection limit of 0.008 mu M for MMHg was achieved. Recovery studies of cell extracts spiked with MMHg were done to check the reliability of the method and in all cases satisfactory recoveries (90-108%) were obtained. Remarkable differences between MMHg levels inside both human glioblastoma and neuroblastoma cell lines were found in initial experiments, which seem to be related to different genotoxic effects. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available