Article
Multidisciplinary Sciences
Yicheng Gong, Chuancun Wei, Jun Wang, Nengjiang Mu, Qinhong Lu, Chengyao Wu, Ning Yan, Huifang Yang, Yao Zhao, Xiuna Yang, Sudagar S. Gurcha, Natacha Veerapen, Sarah M. Batt, Zhiqiang Hao, Lintai Da, Gurdyal S. Besra, Zihe Rao, Lu Zhang
Summary: Arabinogalactan (AG) is an essential component in mycobacterial species, and AftA is a key enzyme involved in AG biosynthesis. The cryo-EM structure of Mtb AftA reveals its dimeric assembly and the participation of a metal ion in its interaction. Structural analysis and mutagenesis studies provide insights into the priming mechanism catalyzed by AftA in Mtb AG biosynthesis, which can contribute to anti-TB drug discovery.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Patrick Niekamp, Gaelen Guzman, Hans C. Leier, Ali Rashidfarrokhi, Veronica Richina, Fabian Pott, Caroline Barisch, Joost C. M. Holthuis, Fikadu G. Tafesse
Summary: Phagocytosis by alveolar macrophages is the initial step in Mycobacterium tuberculosis (Mtb) infection. The process relies on an intact sphingolipid biosynthetic pathway, where disruption leads to reduced pathogen uptake. This pathway is crucial for the segregation of regulatory molecules and downstream activation of signaling molecules, highlighting a potential target for antimycobacterial therapeutics.
Article
Chemistry, Medicinal
Vinayak Singh, Godwin Akpeko Dziwornu, Amanda Mabhula, Kelly Chibale
Summary: This study identified elongation factor G as the target of fusidic acid in Mycobacterium tuberculosis, demonstrating the essentiality of fusA1 gene in M. tuberculosis and validating it as a novel drug target.
ACS INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Felipe B. D'Andrea, Nicholas C. Poulton, Ruby Froom, Kayan Tam, Elizabeth A. Campbell, Jeremy M. Rock
Summary: The Mtb Clp protease system, with its essential ClpP1 proteolytic subunit, serves as a promising noncanonical drug target against Mycobacterium tuberculosis.
Article
Multidisciplinary Sciences
Samantha C. Quintanilla, Neda Habibi Arejan, Parthvi Patel, Cara Boutte
Summary: This study identifies a new polar factor, PlrA, that is responsible for restricting peptidoglycan metabolism at the cell poles in Mycobacterium smegmatis. It is found that only the N-terminal membrane domain of PlrA is necessary for its function. Furthermore, PlrA is shown to regulate the polar foci of the polar growth factor, Wag31.
Article
Cell Biology
Kristina Ritter, Jan Christian Sodenkamp, Alexandra Hoelscher, Jochen Behrends, Christoph Hoelscher
Summary: The study showed that the absence of IL-6 or gp130 on T cells has a minor effect on the development of antigen-specific TH1 and TH17 cells during experimental TB, and these gene-deficient mice were as capable as wild type mice in controlling mycobacterial infection.
Article
Microbiology
Francesca Boldrin, Itxaso Anso, Sogol Alebouyeh, Iker A. Sevilla, Marivi Geijo, Joseba M. Garrido, Alberto Marina, Laura Cioetto Mazzabo, Greta Segafreddo, Marcelo E. Guerin, Riccardo Manganelli, Rafael Prados-Rosales
Summary: The research highlights the vital role of PatA, an acyltransferase involved in the PIM biosynthetic pathway, for the survival and virulence of Mycobacterium tuberculosis. Silencing the patA gene leads to bactericidal effects in vitro and in vivo, underscoring the potential of PatA as a novel drug target against this major human pathogen.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Veterinary Sciences
Lucia Vangone, Lorena Cardillo, Marita Georgia Riccardi, Giorgia Borriello, Anna Cerrone, Paolo Coppa, Roberto Scialla, Emanuela Sannino, Gianluca Miletti, Giorgio Galiero, Giovanna Fusco
Summary: A case of Mycobacterium tuberculosis infection was found in a deceased adult male dog in Southern Italy, showing signs of mistreatment. This is the first report of Mycobacterium tuberculosis SIT42 infection in a dog in Italy.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Tuhina Gupta, Demba Sarr, Kayla Fantone, Nuha Milad Ashtiwi, Kaori Sakamoto, Frederick D. Quinn, Balazs Rada
Summary: Mtb is the primary cause of human tuberculosis, but the role of Duox1 in bacterial infections remains largely unknown. This study showed that in a mouse model, Duox1 is dispensable for the overall clinical course of Mtb lung infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Yoann Rombouts, Olivier Neyrolles
Summary: This study reveals that Mycobacterium tuberculosis produces a virulence factor called 1-tuberculosinyladenosine, which leads to lysosomal dysfunction and lipid accumulation in macrophages similar to lysosomal storage diseases. These findings provide insights into how the bacteria manipulates host lipid metabolism for its survival and suggest new strategies for host-directed therapy against tuberculosis.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Review
Immunology
Cheldon Ann Alcantara, Ira Glassman, Kevin H. Nguyen, Arpitha Parthasarathy, Vishwanath Venketaraman
Summary: Mycobacterium tuberculosis (M. tb) remains a major cause of death in developing countries. The BCG vaccine is widely used in developing countries but its efficacy is still uncertain. Neutrophils play a crucial role in the immune response against M. tb, promoting clearance of the bacteria and regulating the inflammatory response. This review highlights the role of neutrophils during M. tb infection and emphasizes the need for more research on effective vaccination.
Article
Biochemistry & Molecular Biology
Shivangi, Mary Krishna Ekka, Laxman S. Meena
Summary: This study explores the essential factors affecting the enzymatic activity of FtsY, including its hydrolysis kinetics of GTP, the influence of cations like Mg2+ and Mn2+ on activity, and the structural changes of FtsY in response to ligand binding. Mutational analysis showed that mutations at key residues significantly reduce FtsY's GTPase activity, while MD simulation indicated effective binding and stabilization of FtsY with GTP, GDP and ATP.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Shuai Wang, Xiaoyin Cai, Wei Yu, Sheng Zeng, Jingran Zhang, Lingmin Guo, Yamin Gao, Zhili Lu, H. M. Adnan Hameed, Cuiting Fang, Xirong Tian, Buhari Yusuf, Chiranjibi Chhotaray, M. D. Shah Alam, Buchang Zhang, Honghua Ge, Dmitry A. Maslov, Gregory M. Cook, Jiacong Peng, Yongping Lin, Nanshan Zhong, Guoliang Zhang, Tianyu Zhang
Summary: Mycobacterium abscessus is an emerging pathogen that is highly resistant to most antibiotics. This study identified MAB_0189c as a crucial gene responsible for the intrinsic resistance of M. abscessus. Knockout of MAB_0189c decreased cell envelope permeability and sensitized M. abscessus to multiple antibiotics. Additionally, the arabinosyltransferase inhibitor, ethambutol, increased the effectiveness of several drugs against M. abscessus. These findings suggest that targeting MAB_0189c may be a strategy to overcome the intrinsic antibiotic resistance of M. abscessus.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Deodutta Roy, Nasreen Z. Ehtesham, Seyed Ehtesham Hasnain
Summary: The study highlights the potential role of Mycobacterium tuberculosis in inducing lung cancer by sharing key characteristics with established carcinogens. Experimental evidence and mechanistic data support the hypothesis that Mtb could act as a complete human pulmonary carcinogen, suggesting implications for the management and treatment of TB cases with potential cancerous lesions.
Article
Microbiology
Lei Zhang, Ye Zhang, Yuanyuan Li, Fengmin Huo, Xi Chen, Hui Zhu, Shaochen Guo, Lei Fu, Bin Wang, Yu Lu, Alexandra Aubry
Summary: This study reviews and details the discovery of a mutation associated with Clofazimine resistance and provides additional evidence of its link to resistance in 30 additional isolates. This research contributes to a comprehensive understanding of Clofazimine resistance mechanisms, which is critical for reducing resistance emergence and aiding in anti-TB drug discovery.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Eleonore Lepvrier, Laura Mollintraffort, Michael Nigen, Renan Goude, Diane Allegro, Pascale Barbier, Vincent Peyrot, Daniel Thomas, Alexis Nazabal, Cyrille Garnier
ANALYTICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Kevin Mace, Fanny Demay, Charlotte Guyomar, Sylvie Georgeault, Emmanuel Giudice, Renan Goude, Annie Trautwetter, Gwennola Ermel, Carlos Blanco, Reynald Gillet
JOURNAL OF MOLECULAR BIOLOGY
(2017)
Article
Microbiology
R. Goude, A. G. Amin, D. Chatterjee, T. Parish
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2009)
Review
Microbiology
Renan Goude, Tanya Parish
FUTURE MICROBIOLOGY
(2008)
Article
Microbiology
Renan Goude, Anita G. Amin, Delphi Chatterjee, Tanya Parish
JOURNAL OF BACTERIOLOGY
(2008)
Article
Microbiology
Maud Flechard, Catherine Fontenelle, Carlos Blanco, Renan Goude, Gwennola Ermel, Annie Trautwetter
Article
Biochemistry & Molecular Biology
Charlotte Guyomar, Marion Thepaut, Sylvie Nonin-Lecomte, Agnes Mereau, Renan Goude, Reynald Gillet
NUCLEIC ACIDS RESEARCH
(2020)
Article
Hematology
Cyrille Garnier, Fatma Briki, Brigitte Nedelec, Patrick Le Pogamp, Ahmet Dogan, Nathalie Rioux-Leclercq, Renan Goude, Caroline Beugnet, Laurent Martin, Marc Delpech, Frank Bridoux, Gilles Grateau, Jean Doucet, Philippe Derreumaux, Sophie Valleix
Article
Biotechnology & Applied Microbiology
R Goude, S Renaud, S Bonnassie, T Bernard, C Blanco
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2004)
Article
Microbiology
T Touzé, R Goude, S Georgeault, C Blanco, S Bonnassie
JOURNAL OF BACTERIOLOGY
(2004)