Journal
MICROBIOLOGY AND IMMUNOLOGY
Volume 62, Issue 9, Pages 607-611Publisher
WILEY
DOI: 10.1111/1348-0421.12641
Keywords
bacterial vaginosis; Gardnerella vaginalis; loop-mediated isothermal amplification; rapid detection
Categories
Funding
- JSPS KAKENHI
Ask authors/readers for more resources
The aim of this study was to develop a method for the rapid detection of Gardnerella vaginalis, which is proposed to play a key role in the pathogenesis of bacterial vaginosis. Specific loop-mediated isothermal amplification (LAMP) primers were designed and used to detect target DNA within 45min under isothermal conditions. Comparative screening indicated that the LAMP assay is superior to PCR in terms of rapidity, and is equivalent in sensitivity and specificity. This LAMP assay can be used for rapid screening and detection of G. vaginalis in vaginal samples; the limit of detection is 10 fg DNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available