Journal
MICROBIOLOGY AND IMMUNOLOGY
Volume 55, Issue 11, Pages 798-808Publisher
WILEY
DOI: 10.1111/j.1348-0421.2011.00376.x
Keywords
Mycobacterium tuberculosis; vaccine; human Interleukin (IL-12) p70; Early Sectetary Antigen Target 6
Categories
Funding
- Chinese National Key Project of Infectious Disease [2008ZX10003-013]
- NSFC of China [30872257]
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ESAT-6 protein of Mycobacterium tuberculosis is absent in Mycobacterium bovis BCG and Mycobacterium microti and has been demonstrated to stimulate strong cell-mediated immunity. IL-12 can play crucial roles in regulating IFN-gamma production and Th1 effectors production. In this study, we constructed three rBCG vaccines that could express proteins of human IL-12p70 and/or ESAT-6 and evaluated their immunogenicity and protective efficacy in mice. Our experiments illustrated that the rBCG-IE (expressing a fusion protein of human IL-12p70 and ESAT-6) was capable of inducing stronger Th1 type cell-mediated immune responses than conventional BCG, or rBCG-I (expressing human IL-12p70), or rBCG-E (expressing ESAT-6). However, the results of protective experiments showed that rBCG-IE could only confer similar and even lower protective efficacy against M. tuberculosis H37Rv infection compared with BCG vaccine.
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