Journal
MICROBIOLOGY
Volume 82, Issue 4, Pages 404-409Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261713040061
Keywords
microbial fuel cells; NAD(+)-dependent formate dehydrogenase; anaerobic respiration; Shewanella oneidensis MR-1
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An expression plasmid was constructed in order to carry out heterologous expression of the gene of the NAD(+)-dependent formate dehydrogenase (FDH) from methylotrophic bacterium Moraxella sp. in the cells of Shewanella oneidensis MR-1 under aerobic and anaerobic conditions. In both modes of cell cultivation, recombinant FDH activity was revealed in the cell lysate of the transformants. In the medium with laA double dagger tate as a carbon source, the rate of anaerobic respiration determined as the rate of conversion of fumarate (the electron acceptor) to succinate was higher in the transformant with recombinant FDH. Anaerobic cultivation of the FDH-containing transformant of S. oneidensis MR-1 in a microbial fuel cell (MFC) revealed increased current density.
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