Article
Microbiology
Hovik Panosyan, Armine Margaryan, Nils-Kare Birkeland
Summary: Twelve thermophilic Anoxybacillus strains were isolated from a hot spring in Nagorno-Karabakh, with strain K1(T) being Gram-stain-positive, moderately thermophilic, and capable of growth within specific temperature and pH ranges. The strain showed high similarity to Anoxybacillus flavithermus DSM 2641(T) but was proposed as a separate species, Anoxybacillus karvacharensis, based on genetic analysis.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Ai -Ping Lv, Yu-Zhen Ming, Ze-Tao Liu, Yu-Ting Ouyang, Meng -Meng Li, Zheng-Han Lian, Jian-Yu Jiao, Ting-Ting She, Lan Liu, Wen-Jun Li
Summary: A novel anaerobic bacterium SYSU GA19001T, which belongs to the genus Clostridium, was isolated from a hot spring sediment sample. It showed the highest sequence similarity to Clostridium swellfunianum CICC 10730T (96.63%) and Clostridium prolinivorans PYR-10T (96.11%). The strain SYSU GA19001T is a Gram-stain positive, spore-forming, rod-shaped bacterium, and it can grow at pH 5.0-9.0 (optimum, pH 7.0), 37-55°C (optimum, 45°C), and in NaCl concentrations of 0-2.0% (optimum, 0%).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Junia Schultz, Mariana Teixeira Dornelles Parise, Doglas Parise, Laenne G. Medeiros, Thiago J. Sousa, Rodrigo B. Kato, Ana Paula Trovatti Uetanabaro, Fabricio Araujo, Rommel Thiago Juca Ramos, Siomar de Castro Soares, Bertram Brenig, Vasco Ariston de Carvalho Azevedo, Aristoteles Goes-Neto, Alexandre S. Rosado
Summary: This study provides insights into the genomic features and phylogenomics of Anoxybacillus strains inhabiting the Antarctic thermophilic ecosystem. The strains showed high genomic similarity to each other and to A. flavithermus, and possessed various genes related to resistance, metabolism, and temperature adaptation. Moreover, prediction of glycosyl hydrolase enzyme-encoding genes, secondary metabolites, and prophage sequences suggests their potential for biotechnological applications.
Article
Ecology
Yan Zhang, Tao Liu, Meng-Meng Li, Zheng-Shuang Hua, Paul Evans, Yanni Qu, Sha Tan, Min Zheng, Hui Lu, Jian-Yu Jiao, Sebastian Lucker, Holger Daims, Wen-Jun Li, Jianhua Guo
Summary: The recent discovery of comammox Nitrospira species capable of complete ammonia oxidation in non-marine thermal habitats expands our understanding of microbial nitrification. This study reports the wide distribution of comammox Nitrospira in terrestrial hot springs and provides new insights into their metabolic activity and thermal adaptation.
Article
Microbiology
Chao-Jian Hu, Wen-Dong Xian, Yi-Qing Lv, Cheng-Xiang Peng, Rong-Xu Shan, Ze-Chang Cheng, Qian Lv, Ye Tian, Jian-Yu Jiao, Sha Tan, Wen-Jun Li
Summary: Two closely related moderately thermophilic bacteria, strains SYSU G05006T and SYSU G05005, were isolated from a sample collected from Rehai National Park, Tengchong, Yunnan Province, PR China. They were closely related to Caldovatus sediminis based on the phylogenetic analysis and were described as a new species, Caldovatus aquaticus sp. nov. Strain SYSU G05006T had a genome size of 3.87 Mbp and showed optimal growth at 55 degrees C and pH 6.0.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Mohit Kumar Saini, Shohei Yoshida, Aswathy Sebastian, Eri Hara, Hideyuki Tamaki, Nathan T. Soulier, Istvan Albert, Satoshi Hanada, Marcus Tank, Donald A. Bryant
Summary: A new microbial strain, named Elioraea tepida, was isolated from an alkaline siliceous hot spring in Yellowstone National Park, USA. The strain grows chemoheterotrophically by oxygen-dependent respiration and can also undergo photoheterotrophic growth under strictly oxic conditions. It does not exhibit photoautotrophic growth under oxic or anoxic conditions, indicating that it belongs to an aerobic anoxygenic phototrophic bacterium.
Article
Microbiology
Chao-Jian Hu, Wen-Dong Xian, Xiao-Qing Luo, Lan Liu, Meng-Meng Li, Jian-Yu Jiao, Sha Tan, En-Min Zhou, Wen-Jun Li
Summary: In this study, three new strains closely related to the genus Thermus were isolated from a hot spring in southwestern China. The genomic profile and taxonomic status of one of the strains (SYSU G05001(T)) were determined using whole-genome sequencing and a polyphasic taxonomic approach. This strain, designated as Thermus brevis sp. nov., exhibited optimal growth at a temperature of 55 degrees Celsius, pH 7.0, and NaCl concentration of 1%.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Dariimaa Ganbat, DoKyung Oh, Yong-Jik Lee, Dong-Woo Lee, Seong-Bo Kim, Won-Jae Chi, Ki-Eun Lee, Byoung-Hee Lee, You-Jung Jung, Jung-Sook Lee, Sang-Jae Lee
Summary: The taxonomic position of strain EF45031(T) from a hot spring was determined using molecular methods, and it was classified as a new species of the genus Brachybacterium, named Brachybacterium sillae sp. nov.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
Yuxin Chen, Arisa Nishihara, Takao Iino, Moriya Ohkuma, Shin Haruta
Summary: YA01T, a novel nitrogen-fixing fermentative bacterium isolated from Nakabusa hot springs in Japan, represents a new species of the genus Caldicellulosiruptor, with distinct phylogenetic position, biochemical and physiological characteristics, and major cellular fatty acids differentiating it from other Caldicellulosiruptor species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Shigeru Kawai, Joval N. Martinez, Mads Lichtenberg, Erik Trampe, Michael Kuhl, Marcus Tank, Shin Haruta, Arisa Nishihara, Satoshi Hanada, Vera Thiel
Summary: This study identified two different modes of energy metabolism of Chloroflexus aggregans in hot spring microbial mats through metatranscriptomic analyses and microscale measurements, showing that the organism exhibits phototrophy and chemomixotrophy under dynamic environmental conditions.
Article
Biochemistry & Molecular Biology
Anastasiya Matveeva, Alexander Ryabchenko, Viktoria Petrova, Daria Prokhorova, Evgenii Zhuravlev, Alexander Zakabunin, Artem Tikunov, Grigory Stepanov
Summary: Research on Cas9 nucleases from different organisms has the potential to advance genome engineering and gene therapy tools. Thermophilic Cas9 nucleases, which have varying activity temperature ranges and PAM preferences, are expanding. This study focuses on constructing a compact Cas9 nuclease that displays nuclease activity in the 37-60 degrees C range.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Yi Li, Hong-Qian Peng, Li-Quan Yang
Summary: Thermophilic xylanases from hot-spring microorganisms have potential biological and industrial applications, but the high-temperature adaptation mechanisms of these enzymes are still unclear. Molecular dynamics simulations and comparative analysis revealed that XynM1 exhibits smaller structural dynamics and greater thermal stability than XynDRTY1, and temperature-induced structural differences mainly occur in specific regions. These findings contribute to understanding the molecular mechanism of xylanase high-temperature adaptation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Annabella Tramice, Adele Cutignano, Annalaura Iodice, Annarita Poli, Ilaria Finore, Giuseppina Tommonaro
Summary: Anoxybacillus amylolyticus produces N-butanoyl-homoserine lactone, a signal molecule that may regulate extracellular alpha-amylase activity through QS signaling.
Article
Microbiology
Yu-Zhen Ming, Lan Liu, Ai-Ping Lv, Wen-Dong Xian, Ze-Tao Liu, Meng-Meng Li, Jian-Yu Jiao, Bao-Zhu Fang, Wen-Jun Li
Summary: Two bacterial strains, SYSU G02173(T) and SYSU G03142, isolated from hot springs in Tibet, belong to the family Sphingosinicellaceae and exhibit aerobic growth with optimal conditions at neutral pH and higher temperatures. These novel strains showed positive oxidase and catalase activities, along with motile rod-shaped cells and growth with NaCl in the culture medium.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Christaline George, Chloe Xue Qi Lim, Yan Tong, Stephen Brian Pointing
Summary: The Sembawang Hot Spring in Singapore is located at the foot of a geological feature called the Bentong-Raub Suture Zone. The hot spring exhibits a poly-extreme environment and supports distinct microbial communities along an environmental gradient. The high abundance of phototrophic bacteria and the presence of chemoautotrophs and heterotrophs reflect the known thermal ranges and allochthonous leaf input in the hot spring. The findings contribute to the understanding of hot spring microbiomes and address a biogeographic knowledge gap for the region.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Leonid Kulikov, Natalia Inkova, Daria Cherniuk, Anton Teslyuk, Zorigto Namsaraev
Summary: In this paper, a method called Tensor Interpolating Empirical Orthogonal Functions (TIEOF) is proposed to reconstruct missing data from remote sensors. The method relies on high-order tensor decomposition and shows a 69% improvement compared to the current state-of-the-art DINEOF algorithm in various data scenarios.
Article
Engineering, Environmental
Anna A. Melnikova, Anastasiya V. Komova, Ruslan A. Vasilev, Zorigto B. Namsaraev, Kirill V. Gorin
Summary: In this study, the combined application of FeCl3 and Polyethyleneoxide-based cationic flocculant was used for the first time to harvest enrichment culture from a reservoir during eutrophication, achieving a flocculation efficiency of about 90%. FeCl3, PAA, and PEO alone did not demonstrate sufficient flocculation capacity for the enrichment culture containing different types of microalgae.
Article
Microbiology
E. D. Bakhmutova, A. O. Izotova, S. Toshchakov, Z. B. Namsaraev, A. Komova
Summary: Rhodomicrobium sp. strain Az07 was isolated from a brackish canal and found to be more halotolerant than previously described species of the genus. The genome of the strain was sequenced using the Illumina MiSeq system, yielding an assembly with 3,291,400 bp, 106 contigs, and a GC content of 62.7%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
E. D. Bakhmutova, A. O. Izotova, S. Toshchakov, Z. B. Namsaraev, N. Yermolaeva, A. Komova
Summary: This study presents the draft genome sequence of an anoxygenic phototrophic bacterium, Rhodoferax sp. strain U11-2br, isolated from a freshwater mountain lake in the Ulagan Plateau, Altai, Russia. The assembly comprises 4,514,979 bp with a GC content of 59.9%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Anastasia V. Komova, Elizaveta D. Bakhmutova, Anna O. Izotova, Evelina S. Kochetova, Stepan V. Toshchakov, Zorigto B. Namsaraev, Maxim V. Golichenkov, Aleksei A. Korzhenkov
Summary: The genome of the moderately haloalkaliphilic diazotrophic anoxygenic phototrophic bacterium Rhodovulum tesquicola A-36s(T) was analyzed and compared to closely related species. The study found that the genomic features of the three organisms are similar, but there is a significant difference in nitrogenase activity, with Rhodovulum strictum demonstrating the highest activity.
Review
Microbiology
A. A. Melnikova, A. V. Komova, Z. B. Namsaraev
Summary: This study collected scientific reports on cyanobacterial blooms in terrestrial water bodies and coastal seas of Russia from the 20th and early 21st centuries, and compared them with data on fertilizer use in agriculture, volume of polluted wastewaters, and average air temperature. The results suggest that the cyanobacterial bloom problem was most severe during the period of 1930-1991, driven by large reservoir construction, untreated wastewater discharge, and widespread fertilizer use in agriculture. After the collapse of the Soviet Union, there was a reduction in polluted wastewater and fertilizer use, leading to a decrease in bloom intensity. However, the resumption of fertilizer use and a sharp increase in average annual temperatures may be causing an intensification of cyanobacterial blooms in water bodies observed in the early 21st century.
Article
Biology
Darya Zelenina, Natalia Kuzmenkova, Denis Sobolev, Kirill Boldyrev, Zorigto Namsaraev, Grigoriy Artemiev, Olga Samylina, Nadezhda Popova, Alexey Safonov
Summary: High concentrations of nitrogen, phosphorus, and sulfur can result in strong fixation of strontium, uranium, and plutonium in river bottom sediments, promoting phytoplankton growth and biogenic mineral formation. This can be achieved by adding necessary nutrients to polluted water bodies.
Article
Biotechnology & Applied Microbiology
Hongyu Chen, Shengzhou Shan, Chun Wang, Zorigto Namsaraev, Ivan Dubovskiy, Chengxu Zhou, Roger Ruan, Xiaojun Yan, Pengfei Cheng
Summary: This study examined the effects of different carbon and nitrogen sources on the growth of algal cells. It was found that the highest algal cell density was achieved at a glycerol concentration of 5.0 g/L in mixotrophic culture, reaching 5.25*l06 cells/mL. The maximum algal cell density of 6.23*106 cells/mL was obtained when the concentration of nitrogen urea was 0.075 g/L. Additionally, the lipid content significantly increased to 156.5 mg/L under mixotrophic culture.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Pengfei Cheng, Shengzhou Shan, Zhujun Zhu, Kui Liu, Zorigto Namsaraev, Ivan Dubovskiy, Qingshan Xu
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Zorigto Namsaraev, Anna Bobrik, Aleksandra Kozlova, Anastasia Krylova, Anastasia Rudenko, Anastasia Mitina, Aleksandr Saburov, Maksim Patrushev, Olga Karnachuk, Stepan Toshchakov
Summary: Cryogenic soils are important carbon reservoirs, and their microbial diversity has a strong impact on CO2 emissions. Rising temperatures in the high Arctic can activate microbial processes and increase carbon input from these soils. Using high-throughput sequencing, the microbial community composition and diversity were studied in the soil of Barents Sea archipelagos. The study found that soil moisture and temperature were the main factors influencing the microbial community composition, and CO2 emission levels were influenced by local factors such as moisture, microclimate, and animal fecal input from reindeer.
Article
Biology
Zorigto Namsaraev, Aleksandra Kozlova, Fedor Tuzov, Anastasia Krylova, Anna Izotova, Ivan Makarov, Andrei Bezgreshnov, Anna Melnikova, Anna Trofimova, Denis Kuzmin, Maksim Patrushev, Stepan Toshchakov
Summary: The prokaryotic communities in the Barents Sea are strongly affected by climate change and influenced by microbiota from the Atlantic Ocean. This study identified two distinct types of communities and discussed their origin in terms of biogeography.
Review
Nanoscience & Nanotechnology
I. A. Kolesnikov, M. V. Nikolenko, A. V. Ermakov, A. A. Korzhenkov, A. A. Zaikin, V. E. Velikhov, S. A. Bobkov, F. S. Sharko, Z. B. Namsaraev, M. V. Patrushev
Summary: This article provides information on the National Genomic Information Database (NGID) being created by the National Research Center Kurchatov Institute in Russia. The NGID is planned to be a crucial component of the genetic research and development infrastructure in Russia, allowing for the storage, integration, and analysis of genetic data. It is mandatory for organizations involved in genetic engineering to deposit information in the NGID.
NANOBIOTECHNOLOGY REPORTS
(2023)
Review
Nanoscience & Nanotechnology
M. V. Patrushev, A. A. Borisova, Z. B. Namsaraev
Summary: Synthetic biology is an interdisciplinary field that aims to design and construct living systems at various levels through the application of engineering principles. This review discusses the main characteristics of synthetic biology and the modern methods used by synthetic biologists to create new biological systems and components. It also emphasizes the actions needed to promote the development of synthetic biology in Russia.
NANOBIOTECHNOLOGY REPORTS
(2023)
Article
Nanoscience & Nanotechnology
M. A. Staronenkova, T. V. Chikhalina, Z. B. Namsaraev
Summary: Industrial enzymes, bacterial and yeast starter cultures are highly sought-after products in modern genetic technologies, playing a crucial role in the food industry. Russia, as one of the largest food producers in the world, heavily relies on imported enzyme and microbial cultures in various industries.
NANOBIOTECHNOLOGY REPORTS
(2023)
Review
Nanoscience & Nanotechnology
Z. B. Namsaraev, A. A. Korzhenkov, D. Yu. Fedosov, M. V. Patrushev
Summary: The development of digital biological information brings great opportunities and challenges, and it is necessary to find the optimal balance for further growth of digital sequence information while ensuring the security of personal data and complying with legal and ethical standards.
NANOBIOTECHNOLOGY REPORTS
(2023)