Journal
MICROBIOLOGY
Volume 77, Issue 5, Pages 553-558Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S002626170805007X
Keywords
Paenibacillus polymyxa; exopolysaccharides; viscosity; monosaccharide composition; immunodiffusion analysis
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Exopolysaccharides (EPS) synthesized by Paenibacillus polymyxa 1465 in the course of batch cultivation were proven to contain neutral and acidic fractions. EPS are heterogeneous polysaccharides, represented by a complex of macromolecules with molecular mass of 7 x 10(4) to 2 x 10(6) Da. The acidic component was shown to be predominant in EPS preparations isolated from bacteria cultivated on glucose, which corresponds to a higher viscosity of EPS water solutions. The exoglycans were shown to contain glucose, mannose, galactose, and uronic acids. Polyclonal rabbit antibodies against the isolated P. polymyxa 1465 EPS preparations were used in a comparative immunodiffusion analysis of a number of P. polymyxa strains.
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