Article
Engineering, Environmental
Mingchang Li, Jiaqi Yu, Lu Cao, Yujun Yin, Zhaoying Su, Shuai Chen, Guoqiang Li, Ting Ma
Summary: This study demonstrates that thermophilic facultative anaerobic bacteria can produce bioemulsifiers with excellent emulsifying capability and resilience, providing potential applications in bioremediation and enhanced oil recovery.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Hyun-Song Kim, Jung-A Choi, Bu-Yeon Kim, Lenny Ferrer, Jung-Min Choi, Volker F. Wendisch, Jin-Ho Lee
Summary: Aromatic aldehydes, such as 4-HB aldehyde, PC aldehyde, and vanillin, are widely used in flavors, fragrances, and pharmaceutical precursors. However, their production in microbial hosts is hindered by rapid reduction to alcohols. In this study, the NCgl0324 gene was identified to possess aromatic aldehyde reductase activity, and its deletion led to increased production of 4-HB aldehyde, PC aldehyde, and vanillin in microbial strains. These findings highlight the potential of modulating the NCgl0324 gene to enhance the production of valuable aromatic aldehydes and alcohols.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Nagarajan Siddharthan, Ramasamy Balagurunathan, Srinivasan Venkatesan, Natarajan Hemalatha
Summary: Microbial interactions with plant hosts play a significant role in plant growth and productivity. In this study, the insecticidal and antifungal effects of the Geobacillus thermodenitrificans PS41 strain were investigated. The secondary metabolites of PS41 showed potential for controlling Spodoptera litura larvae and inhibiting plant pathogenic fungi. Moreover, PS41 did not exhibit toxicity towards earthworms and supported plant growth. It also did not show antagonistic activity against soil bacteria. These findings highlight the potential of PS41 as an eco-friendly organic candidate for crop production.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Vandierly Sampaio de Melo, Brisa Moreira Gomes, Felipe Santiago Chambergo
Summary: In this study, a beta-xylosidase from Geobacillus thermodenitrificans (GtXyl) was expressed and characterized. The enzyme showed the highest activity at pH 5.0 and a temperature of 60°C. It exhibited stability in the presence of metal ions and xylose, and also presented bifunctional activity of beta-xylosidase and alpha-L-arabinofuranosidase.
CARBOHYDRATE RESEARCH
(2023)
Article
Immunology
Li-Ping Zhu, Wei-Lan Yin, Lei Peng, Xiao-Hong Zhou, Peng Zhou, Shu-Xia Xuan, Ying Luo, Chen Chen, Bin Cheng, Jin-Duan Lin, Yan-Mei Liu, Feng-Jun Tan, Wei-Guo Yin
Summary: The study revealed a correlation between the Glu504Lys polymorphism in the ALDH2 gene and myocardial infarction (MI), with the genotype GA/AA being an independent risk factor for MI. Alcohol consumption, high myoglobin levels, and low levels of high-density lipoprotein cholesterol were also significantly associated with a higher incidence of MI.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Siddharthan Nagarajan, Balagurunathan Ramasamy, Hemalatha Natarajan
Summary: Feather waste can be effectively converted into bioactive peptides using keratinase producing bacteria, which exhibit antioxidant, antibacterial, anticancer, and wound healing properties.
PROCESS BIOCHEMISTRY
(2023)
Review
Cardiac & Cardiovascular Systems
Junyan Jin, Jieying Chen, Yaping Wang
Summary: This review summarizes the potential role of ALDH2 activation in antiarrhythmic protection, the correlation between the ALDH2*2 polymorphism and arrhythmic risk, and discusses important new findings on the use of ALDH2 activators as promising antiarrhythmic therapy agents.
Article
Chemistry, Physical
Hubert Casajus, Aurelie Lagarde, Lionel Nauton, Nazim Ocal, Martin Leremboure, Wolf-Dieter Fessner, Nicolas Duguet, Franck Charmantray, Laurence Hecquet
Summary: This study demonstrates the successful cleavage of aliphatic alpha-hydroxy ketones using thermostable transketolase variants obtained through rational design, offering a promising biocatalytic strategy as a one-step alternative for cleavage reactions.
Article
Chemistry, Physical
Hubert Casajus, Aurelie Lagarde, Lionel Nauton, Nazim Ocal, Martin Leremboure, Wolf-Dieter Fessner, Nicolas Duguet, Franck Charmantray, Laurence Hecquet
Summary: Thermostable transketolase variants from Geobacillus stearothermophilus obtained through rational design can be used in vitro to cleave aliphatic alpha-hydroxy ketones, with high activity and yield.
Article
Medicine, General & Internal
Ichiro Wakabayashi, Harald Sourij, Yoko Sotoda, Takashi Daimon, Klaus Groschner, Peter P. Rainer
Summary: There were no significant differences in serum levels of ALDH2- and ADH1B-related miRNAs between Austrian and Japanese middle-aged men. However, miR-150-3p and miR-4314 showed higher expression levels in Austrian men compared to Japanese men, indicating they may play a role in the ethnic difference in sensitivity to alcohol through modifying the expression of ADH1B.
JOURNAL OF CLINICAL MEDICINE
(2021)
Editorial Material
Biochemistry & Molecular Biology
Jolanta Floryszak-Wieczorek, Ewa Sobieszczuk-Nowicka, Magdalena Arasimowicz-Jelonek
Summary: Nitroxyl (HNO), a one-electron reduced and protonated congener of nitric oxide (NO), has been recently discovered in Arabidopsis thaliana. Due to its distinct chemical properties, further studies are needed to determine the impacts of HNO on physiological processes.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Che-Hong Chen, Benjamin R. Kraemer, Lucia Lee, Daria Mochly-Rosen
Summary: Human ALDH is a multigene family with 19 functional members involved in detoxification and biotransformation. Genetic mutations can lead to toxic aldehyde accumulation and human pathologies. The physiological functions and substrate specificity of many ALDH genes are still unknown.
Article
Biotechnology & Applied Microbiology
Jean-Philippe Kanter, Philipp Jakob Honold, David Lueke, Sven Heiles, Bernhard Spengler, Marco Alexander Fraatz, Christoph Harms, Jakob Peter Ley, Holger Zorn, Andreas Klaus Hammer
Summary: Aldehydes can be produced by converting fatty acids with enzymes from the alpha-dioxygenase pathway. The enzymes alpha-dioxygenase (alpha-DOX) from Crocosphaera subtropica and fatty aldehyde dehydrogenase (FALDH) from Vibrio harveyi were used in a tandem repetitive reaction to achieve high aldehyde yields. The method was then applied to sea buckthorn pulp oil to produce flavoring/fragrance ingredients. The resulting aldehydes were identified, characterized, and analyzed for their odor impressions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Jichao Xiao, John Montgomery
Summary: A simple procedure for the nickel-catalyzed defluorinative alkylation of unactivated aliphatic aldehydes is reported. The protocol exhibits broad substrate scope, mild conditions, and a simple catalytic setup.
Article
Microbiology
Nagarajan Siddharthan, Ramasamy Balagurunathan, Natarajan Hemalatha
Summary: In this study, a novel feather-degrading Geobacillus thermodenitrificans PS41 bacterial isolate was identified from poultry farm soil. The isolated bacterial isolates showed significant feather degradation with keratinase enzyme production, indicating their potential in biodegradation of feather waste.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shuang Yang, Daoyi Xi, Fuyi Jing, Deju Kong, Junli Wu, Lu Feng, Boyang Cao, Lei Wang
CANADIAN JOURNAL OF MICROBIOLOGY
(2018)
Article
Multidisciplinary Sciences
Xiaojun Zhang, Jianbo Yuan, Yamin Sun, Shihao Li, Yi Gao, Yang Yu, Chengzhang Liu, Quanchao Wang, Xinjia Lv, Xiaoxi Zhang, Ka Yan Ma, Xiaobo Wang, Wenchao Lin, Long Wang, Xueli Zhu, Chengsong Zhang, Jiquan Zhang, Songjun Jin, Kuijie Yu, Jie Kong, Peng Xu, Jack Chen, Hongbin Zhang, Patrick Sorgeloos, Amir Sagi, Acacia Alcivar-Warren, Zhanjiang Liu, Lei Wang, Jue Ruan, Ka Hou Chu, Bin Liu, Fuhua Li, Jianhai Xiang
NATURE COMMUNICATIONS
(2019)
Correction
Microbiology
Lingyan Jiang, Lu Feng, Bin Yang, Wenwen Zhang, Peisheng Wang, Xiaohan Jiang, Lei Wang
Review
Microbiology
Bin Liu, Axel Furevi, Andrei Perepelov, Xi Guo, Hengchun Cao, Quan Wang, Peter R. Reeves, Yuriy A. Knirel, Lei Wang, Goran Widmalm
FEMS MICROBIOLOGY REVIEWS
(2020)
Article
Biochemistry & Molecular Biology
Dalong Hu, Bin Liu, Lei Wang, Peter R. Reeves
MOLECULAR BIOLOGY AND EVOLUTION
(2020)
Article
Chemistry, Applied
Xiaolong Jiang, Jing Bai, Jian Yuan, Huijing Zhang, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Lei Wang, Di Huang, Lu Feng
Summary: By reprogramming the metabolic network, the research team increased the yield of OPS-based glycoconjugate vaccines to 38.6 mg L-1, the highest reported so far. Mass spectrometry analysis showed a high glycosylation efficiency of the designed glycosylation sequon in CTB-O5 and CTB-O7, demonstrating the effectiveness of the glyco-optimized platform in producing vaccines against ExPEC.
CARBOHYDRATE POLYMERS
(2021)
Article
Immunology
Shujie Li, Yu Pang, Si Zhang, Qing Li, Qian Wang, Lei Wang, Lu Feng
Summary: This study provides the first comprehensive analysis of the transcriptome profile of UPEC during its intra-BECs life, revealing a new virulence-associated gene and its regulatory mechanism.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
Summary: S. Typhimurium infection induces upregulated glycolysis and decreased serine synthesis in host macrophages, leading to accumulation of glycolytic intermediates that promote bacterial replication and virulence.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yutao Liu, Bin Liu, Tingting Xu, Qian Wang, Wendi Li, Jialin Wu, Xiaoyu Zheng, Bin Liu, Ruiying Liu, Xingmei Liu, Xi Guo, Lu Feng, Lei Wang
Summary: The study identifies a fructose uptake system crucial for the growth of Vibrio cholerae in seawater, where fructose concentrations are low. This system may be important for the survival of pandemic V. cholerae in seawater.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Bin Liu, Chengqian Qian, Pan Wu, Xiaodan Li, Yutao Liu, Huiqian Mu, Min Huang, Yang Zhang, Tianyuan Jia, Yuanyuan Wang, Lu Wang, Xiao Zhang, Di Huang, Bin Yang, Lu Feng, Lei Wang
Summary: Enterohemorrhagic Escherichia coli regulates its cell wall structure during infection, balancing survival in the intestinal lumen and infection of intestinal epithelial cells. The bacteria express long-chain OAg in the intestinal lumen for increased resistance to antimicrobial peptides, but reduce OAg chain length upon sensing epithelial cell attachment, leading to enhanced injection of T3SS effectors into epithelial cells for host cell infection. This regulatory mechanism may be widespread in pathogenic bacteria and closely related to bacterial pathogenesis.
Article
Food Science & Technology
Min Wang, Bin Liu, Yanli Xu, Xi Guo, Tian Fu, Jiafei Liu, Lei Wang
Summary: In this study, the O-antigen gene clusters of V. fluvialis were analyzed by whole-genome sequencing, and a microsphere-based suspension array (MSA) was developed for the identification of different serogroups. Additionally, 13 new O-antigen gene cluster types were discovered. This method provides a rapid and efficient tool for detecting and identifying V. fluvialis serogroups, with potential applications in epidemiological investigations and food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Cell Biology
Yu Pang, Zhihui Cheng, Si Zhang, Shujie Li, Xueping Li, Xiaodan Li, Xiao Zhang, Xiaoxiao Li, Yingxing Feng, Heting Cui, Zhen Chen, Le Liu, Qing Li, Jianxiao Huang, Mingqing Zhang, Siwei Zhu, Lei Wang, Lu Feng
Summary: Urinary tract infections are mainly caused by uropathogenic Escherichia coli (UPEC). This study reveals that UPEC senses host immune responses and initiates escape by upregulating a key phospholipase. The findings provide insights into pathogenic bacteria's response to intracellular phosphate shortage and tackling host defense, and offer potential for developing new therapeutic agents to treat UPEC infection.
Article
Cell Biology
Lu Feng, Bin Yang, Yongchang Xu, Yingying Xiong, Fang Wang, Bin Liu, Wen Yang, Ting Yao, Lei Wang
Summary: This study reveals a signal transduction pathway for the activation of virulence in EHEC O157:H7, providing insights into the relationship between adherence, gene regulation, and pathogenicity.
Article
Cell Biology
Hao Sun, Xuehua Wan, Yu Fan, Peng Liu, Yajun Song, Ningyu Zhu, Zhifeng Duan, Qian Wang, Fang Chen, Changhong Zhou, Yangyang Zheng, Peng Ding, Fenxia Liu, Lu Feng, Kwang Sik Kim, Lei Wang
Summary: The immune response of brain cells to invading bacteria and the mechanism used by pathogenic bacteria to escape immune surveillance in the brain are still not well understood. Microglia can engulf pathogenic bacteria and activate astrocytes to produce C3 for antibacterial activity. Pathogenic bacteria can sense low concentration of threonine in cerebrospinal fluid to reduce the immune response.
Article
Multidisciplinary Sciences
Bin Liu, Yutao Liu, Bin Yang, Qian Wang, Xingmei Liu, Jingliang Qin, Ke Zhao, Fan Li, Xiaohui Feng, Linxing Li, Pan Wu, Miaomiao Liu, Siwei Zhu, Lu Feng, Lei Wang
Summary: This study reveals that EHEC O157:H7 acquired a previously uncharacterized regulatory system rbfSR, which senses riboflavin produced by gut microbiota and activates the expression of virulence genes. This acquisition represents an essential step in the evolution of the highly pathogenic O157:H7. The introduction of rbfSR into other EHEC serotypes also increased their pathogenicity, indicating a potential public health issue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Vera Analise Schommer, Mateus Torres Nazari, Flavia Melara, Julia Catiane Arenhart Braun, Alan Rempel, Lara Franco dos Santos, Valdecir Ferrari, Luciane Maria Colla, Aline Dettmer, Jeferson Steffanello Piccin
Summary: This review introduces the techniques and mechanisms of bacteria immobilization on biochar, as well as its applications in bioremediation and agriculture. The immobilization characteristics of biochar depend on pyrolysis methods, raw materials, and properties of biochar. Scanning electron microscope and colony forming unit analysis are commonly used to verify the immobilization efficiency. Applying biochar-immobilized bacteria can improve soil quality, plant growth, and crop yield.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Manoj Kumar Solanki, Naveen Chandra Joshi, Prashant Kumar Singh, Sandeep Kumar Singh, Gustavo Santoyo, Lucas Carvalho Basilio de Azevedo, Ajay Kumar
Summary: The rhizosphere microbiome plays a crucial role in plant growth and health. Rhizosphere engineering can be used to modify the microbiome and improve plant productivity and resilience.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xin Cao, Xing-Wang Cheng, Yin-Ying Liu, Hong-Wei Dai, Ren-You Gan
Summary: Maintaining oral health is crucial for overall well-being. Imbalances in oral microorganisms can lead to various infectious diseases. Exploring alternatives for prevention and management of oral infections is important due to limitations of current treatments. Natural products offer potential as reliable, effective, and affordable therapeutic options for oral infectious diseases.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Jose David Flores-Felix, Ana Carolina Goncalves, Sara Meirinho, Ana Raquel Nunes, Gilberto Alves, Cristina Garcia-Viguera, Diego A. Moreno, Luis R. Silva
Summary: The application of bacterial biofortifiers is increasing and has been shown to improve the nutritional characteristics of blueberries in Portugal.
MICROBIOLOGICAL RESEARCH
(2024)
Review
Microbiology
Asma Sani, Wan-Qi Qin, Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Shi-Zhong Yang, Bo-Zhong Mu
Summary: This article summarizes the structural diversity of microbial lipopeptide biosurfactants and their applications as biocontrol agents in plants. Lipopeptides have low toxicity and wide range of applications, making them important in the pesticide industry.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xiao-Yu Sun, Jie Deng, Chenhui Zhang, Sin-Yee Fung, Kam-Leung Siu, Ying-Ying Cheng, Liumei Ye, Jiaoxia Qin, Ke Wang, Jiu-Xin Qu, Wenying Gao, Fuxiang Wang, Dong-Yan Jin, Liang Yang
Summary: In this study, a novel c-di-GMP binding protein SodA that regulates oxidative stress tolerance in S. maltophilia was identified. The enzyme activity of SodA was shown to be directly modulated by c-di-GMP through various in vivo and in vitro experiments.
MICROBIOLOGICAL RESEARCH
(2024)
