Journal
MICROBIOLOGICAL RESEARCH
Volume 165, Issue 4, Pages 329-335Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2009.06.001
Keywords
Phytase; Lactobacillus casei; Gene expression; Biochemical characteristics
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Funding
- Natural Science Foundation of China [30571346]
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A 1.4-kb DNA containing the coding region of phytase gene from Aspergillus ficuum (A. ficuum) was connected with the plasmid of plA beta 8 to construct shuffling vector, which was inserted into Lactobacillus casei (L. casei) by electroporation. The Lactobacillus with phytase gene was selected and incubated in anaerobic liquid medium. The results indicated that the highest phytase activities in the supernatant and cells were 22.12 and 4.49 U ml(-1) (P<0.05) at the fourth day of incubation; the optimum pH and temperature of phytase were 5.0 and 40-80 degrees C, respectively (P<0.05); the lowest pH value in the anaerobic medium was 3.35 (P<0.05) at the third day incubation; and the molecular weight of the expressed phytase was 39.2 kDa. (c) 2009 Elsevier GmbH. All rights reserved.
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