Article
Food Science & Technology
Xin Shen, Weicheng Li, Hongyu Cai, Shuai Guo, Min Li, Yangshuo Liu, Zhihong Sun
Summary: This study analyzed the fermentation capacity and metabolic profiles of 17 L. lactis subsp. lactis strains and found significant metabolic differences between strains with different fermentation rates. Peptides, esters, and tributyrin were identified as biomarkers to distinguish between fast and slow fermentation groups.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agriculture, Multidisciplinary
Bibek Byanju, Swastik Sen, Thomas Mansell, Buddhi P. P. Lamsal
Summary: Corn steep liquor (CSL) and thin stillage were evaluated as growth media for recombinant Lactococcus lactis to produce antifreeze proteins (AFPs). Light CSL and thin stillage were optimized with additives and trace elements to enhance the growth of wild-type and recombinant strains. The fermentation supernatants showed longer times to supercool and freeze, indicating potential use as antifreeze compounds in frozen food and non-food applications.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agriculture, Dairy & Animal Science
Xin Song, Lu Liu, Xin-Xin Liu, Zhi-Qiang Xiong, Chun-Liang Xie, Shij-Jie Wang, Lian-Zhong Ai
Summary: This study established a precise and efficient genome editing plasmid for Lactococcus lactis NZ9000 using CRISPR-Cas9 technology, enabling efficient gene deletion and editing. The tool showed high efficiency in gene deletion and sequential gene deletion, providing a new means for further investigation of the L. lactis genome.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Ke Li, Ning Gao, Jiaojiao Tang, Huiqin Ma, Jiayan Jiang, Yufan Duan, Zongjun Li
Summary: Canned bamboo shoots in clear water can produce a unique flavor through bacterial diversity during the fermentation process. The most abundant free amino acid is tyrosine, which has a negative correlation with Lactococcus. Lactococcus lactis TJJ2 can decompose tyrosine and phenylalanine to produce benzaldehyde and benzene acetaldehyde, providing fermented bamboo shoots with a grassy aroma.
Review
Biotechnology & Applied Microbiology
Lejaniya Abdul Kalam, Michelle Yee Mun, Yu Hsuan, Lionel Lian Aun, Liew Phing
Summary: Fermented foods are becoming increasingly popular due to their health-promoting properties. Lactococcus lactis, with its unique fermentation process, plays a key role in the production of dairy products and exhibits beneficial biological activities, such as anti-inflammatory and immunomodulatory effects. It can enhance growth performance, regulate amino acid profiles, improve intestinal immunology and microbiota, and increase infection clearance rate. L. lactis is widely used in the food industry and is being explored for biotechnological applications. In this study, the immunomodulatory effects of different L. lactis strains were reviewed. (c) 2022, The Society for Biotechnology, Japan. All rights reserved.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Nathalie N. S. E. Henriksen, Mads Frederik Hansen, Heiko T. Kiesewalter, Jakob Russel, Joseph Nesme, Kevin R. Foster, Birte Svensson, Gunnar Oregaard, Jakob Herschend, Mette Burmolle
Summary: This study shows that cultivation in a structured environment facilitates coexistence, evolution, and adaptation of bacterial communities. Co-cultivation in a biofilm model resulted in stable coexistence of two bacterial species and drove the evolution of high-yield variants. This study emphasizes the importance of structured environments for the cultivation and evolution of industrially important bacterial communities.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Biotechnology & Applied Microbiology
Adilene Mendoza-Salazar, Lourdes Santiago-Lopez, Maria J. Torres-Llanez, Adrian Hernandez-Mendoza, Belinda Vallejo-Cordoba, Andrea M. Liceaga, Aaron F. Gonzalez-Cordova
Summary: This study evaluated the antioxidant and ACEI activity of edible insect flours fermented with specific Lactococcus lactis strains. The results showed that fermented grasshopper flours demonstrated higher antioxidant activity and ACEI inhibition compared to mealworm flours. Fermentation increased protein hydrolysis, total polyphenol content, and peptide profile, providing potential bioactive compounds for antioxidant and antihypertensive purposes.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Rintaro Sato, Motoyuki Ikeda, Tomonari Tanaka, Hitomi Ohara, Yuji Aso
Summary: This study demonstrates the production of R- and S-1,2-propanediol using engineered Lactococcus lactis for the first time. The addition of mannitol and gluconate in the culture medium enhances 1,2-propanediol production, and a pH-stat approach is used to achieve high concentration of S-1,2-propanediol.
Review
Biochemistry & Molecular Biology
Michael Sauer, Nam Soo Han
Summary: Lactic acid bacteria (LAB) are a specialized group of bacteria found in the gut, food fermentations, and plants, with potential industrial applications. They are being studied for their use in food production, probiotics, therapeutics, and as cell factories for various products.
MICROBIAL CELL FACTORIES-BOOK
(2021)
Article
Agricultural Engineering
Liuyan Gu, Xinxin Xiao, Sang Yup Lee, Bin Lai, Christian Solem
Summary: Anodic electro-fermentation (AEF) shows great potential for using an anode as electron acceptor. In this study, a Lactococcus lactis strain and its adaptively evolved mutant were investigated using a bioelectrochemical system setup. The mutant showed significantly faster growth and achieved a record high current density, demonstrating the potential of AEF.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Fan Suo, Jianming Liu, Jun Chen, Xuanji Li, Christian Solem, Peter R. Jensen
Summary: Selecting a suitable microorganism is crucial for developing economically feasible bioprocesses. Engineering Lactococcus lactis into an efficient microbial platform for producing pyruvate can be successful, while converting lactose into pyruvate can enhance waste valorization.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Ye-Rang Yun, Moeun Lee, Jung Hee Song, Eun Ji Choi, Ji Yoon Chang
Summary: Lactic acid bacteria isolated from kimchi have beneficial effects such as antioxidant, anti-obesity, anti-cancer, and immunomodulatory effects. The fermented vegetable juice showed immunomodulatory effects in vitro and in vivo, indicating its potential use in health promoting fermented foods.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Chemistry, Applied
Min Li, Weicheng Li, Dongyu Li, Juanjuan Tian, Luyao Xiao, Lai-Yu Kwok, Wei Li, Zhihong Sun
Summary: Exopolysaccharides (EPSs) from Lactococcus lactis subsp. lactis IMAU11823 were found to have strong reducing power and no cytotoxicity. The EPSs had different sugar compositions and molecular weights, with EPS-1 being a linear structure composed of glucose and mannose, and EPS-2 being a more complex, non-linear structure composed of mannose, glucose, and rhamnose. Furthermore, a biosynthesis model for EPS production in the IMAU11823 strain was proposed. This study broadens the understanding of the formation, structure, and function of complex EPSs produced by Lactococcus lactis subsp. lactis IMAU11823.
Article
Agriculture, Dairy & Animal Science
Chen Chen, Jiajie Yuan, Haiyan Yu, Bei Wang, Juan Huang, Haibin Yuan, Zhiyuan Xu, Shanshan Zhao, Huaixiang Tian
Summary: This study investigated the genetic and phenotypic association of different Lactococcus lactis strains in the biosynthesis of 3-methylbutanal, revealing the impact of diverse genotypes and gene expression levels on 3-methylbutanal production through both direct and indirect pathways.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Oncology
Katarzyna Kaczmarek, Jerzy Wieckiewicz, Kazimierz Weglarczyk, Maciej Siedlar, Jarek Baran
Summary: This study investigated the use of genetically modified Lactococcus lactis bacteria for local delivery of human soluble TRAIL in CRC treatment. The results showed selective elimination of CRC cells and enhanced anti-tumor activity with oral administration of Metformin.
Article
Microbiology
Vera Analise Schommer, Mateus Torres Nazari, Flavia Melara, Julia Catiane Arenhart Braun, Alan Rempel, Lara Franco dos Santos, Valdecir Ferrari, Luciane Maria Colla, Aline Dettmer, Jeferson Steffanello Piccin
Summary: This review introduces the techniques and mechanisms of bacteria immobilization on biochar, as well as its applications in bioremediation and agriculture. The immobilization characteristics of biochar depend on pyrolysis methods, raw materials, and properties of biochar. Scanning electron microscope and colony forming unit analysis are commonly used to verify the immobilization efficiency. Applying biochar-immobilized bacteria can improve soil quality, plant growth, and crop yield.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Manoj Kumar Solanki, Naveen Chandra Joshi, Prashant Kumar Singh, Sandeep Kumar Singh, Gustavo Santoyo, Lucas Carvalho Basilio de Azevedo, Ajay Kumar
Summary: The rhizosphere microbiome plays a crucial role in plant growth and health. Rhizosphere engineering can be used to modify the microbiome and improve plant productivity and resilience.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xin Cao, Xing-Wang Cheng, Yin-Ying Liu, Hong-Wei Dai, Ren-You Gan
Summary: Maintaining oral health is crucial for overall well-being. Imbalances in oral microorganisms can lead to various infectious diseases. Exploring alternatives for prevention and management of oral infections is important due to limitations of current treatments. Natural products offer potential as reliable, effective, and affordable therapeutic options for oral infectious diseases.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Jose David Flores-Felix, Ana Carolina Goncalves, Sara Meirinho, Ana Raquel Nunes, Gilberto Alves, Cristina Garcia-Viguera, Diego A. Moreno, Luis R. Silva
Summary: The application of bacterial biofortifiers is increasing and has been shown to improve the nutritional characteristics of blueberries in Portugal.
MICROBIOLOGICAL RESEARCH
(2024)
Review
Microbiology
Asma Sani, Wan-Qi Qin, Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Shi-Zhong Yang, Bo-Zhong Mu
Summary: This article summarizes the structural diversity of microbial lipopeptide biosurfactants and their applications as biocontrol agents in plants. Lipopeptides have low toxicity and wide range of applications, making them important in the pesticide industry.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xiao-Yu Sun, Jie Deng, Chenhui Zhang, Sin-Yee Fung, Kam-Leung Siu, Ying-Ying Cheng, Liumei Ye, Jiaoxia Qin, Ke Wang, Jiu-Xin Qu, Wenying Gao, Fuxiang Wang, Dong-Yan Jin, Liang Yang
Summary: In this study, a novel c-di-GMP binding protein SodA that regulates oxidative stress tolerance in S. maltophilia was identified. The enzyme activity of SodA was shown to be directly modulated by c-di-GMP through various in vivo and in vitro experiments.
MICROBIOLOGICAL RESEARCH
(2024)
