Journal
MICROBIOLOGICAL RESEARCH
Volume 164, Issue 1, Pages 105-113Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2006.10.006
Keywords
Plackett - burman design; Response surface methodology; Actinomycetes; Streptomyces psammoticus; Laccase
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Funding
- Council of Scientific and Industrial Research, Government of India
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Response surface methodology was employed for the optimization of different nutritional and physical parameters for the production of laccase by the filamentous bacteria Streptomyces psammoticus MTCC 7334 in submerged fermentation. Initial screening of production parameters was performed using a Plackett - Burman design and the variables with statistically significant effects on laccase production were identified. Incubation temperature, incubation period, agitation rate, concentrations of yeast extract, MgSO(4)7H(2)O, and trace elements were found to influence laccase production significantly. These variables were selected for further optimization studies using a Box-Behnken design. The statistical optimization by response surface methodology resulted in a three-fold increase in the production of laccase by S. psammoticus MTCC 7334. (C) 2006 Etsevier GrnbH. All rights reserved.
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