Journal
MICROBIOLOGICAL RESEARCH
Volume 164, Issue 6, Pages 674-679Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2009.03.001
Keywords
Allochromatium vinosum; Membrane-bound hydrogenase; Gene cloning; Deletion of hydrogen-uptake system; Biological hydrogen production
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Funding
- National 863 project of China [2006AA05Z111]
- NSFC National Foundation for Fostering Talents of Basic Science [J0630649, 30470395]
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Hydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H-2-uptake hydrogenase and H-2-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vinosum was cloned and sequenced. It consist of two structural genes (hydS, hydL) and two intergenic genes (isp1, isp2), which are therefore organized as hydS-isp1-isp2-hydL. This is different from the arrangement of other typical hydrogenase gene clusters. A deletion mutant-strain Phi hydSL, tacking isp1, isp2, partial hydS and hydL genes, was constructed by marker-exchange mutagenesis. Under dark fermentative conditions, the hydrogen production yield by this mutant increased by 62%. The result suggests that the disruption of MBH could greatly improve the hydrogen production in the cells by decreasing the hydrogen uptake. (C) 2009 Elsevier GmbH. All rights reserved.
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