Journal
MICROBIOLOGICAL RESEARCH
Volume 163, Issue 2, Pages 215-224Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2006.05.006
Keywords
Macrophomina phaseolina; pathogenic variations; charcoal rot; sorghum; chlorate phenotype; RAPD fingerprinting
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disease in sorghum grown during the post rainy season in India. Variations in random amplified polymorphic DNA (RAPD) potymorphisms, chlorate sensitivity and pathogenicity were studied among sorghum isolates of M. phaseolina collected from different parts of India. RAPD data based on 14 random primers of Kit A and C (OPA and OPC) on 20 isolates showed a high degree of polymorphism (98.1%) in different isolates. UPGMA dendrogram on RAPD data produced 7 clusters at the level of 37% similarity. Isolates from the same locations showed a tendency to group closer, substantiating closer genetic relatedness. Sorghum infecting Macrophomina isolates showed a mixed response for sensitivity to potassium chlorate (120mM). Chlorate-resistant isolates were predominant (>65% of the isolates) over sensitive isolates. Chlorate-sensitive isolates were found to be genetically closer among them than the resistant ones. For the first time it was shown that chlorate sensitivity in Macrophomina had some relations with charcoal rot severity in sorghum. (C) 2006 Elsevier GmbH. All rights reserved.
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