Article
Microbiology
Md Taj Shafi, Tanvir Bamra, Sushmita Das, Ashish Kumar, Kumar Abhishek, Manjay Kumar, Vinod Kumar, Ajay Kumar, Rimi Mukherjee, Abhik Sen, Pradeep Das
Summary: This study investigated the role of mevalonate kinase in Leishmania survival under stress conditions, revealing its crucial role in protecting the parasite from oxidative stress and potentially contributing to drug unresponsiveness, which could aid in future drug design for leishmaniasis.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Microbiology
Kai Zhang
Summary: Leishmania parasites rely on different sources of lipids during their life cycle stages in the sand fly vector and vertebrate host, reflecting their growth rate and metabolic activity. Future studies may reveal how Leishmania uptake and remodel host lipids at cellular and molecular levels, and blocking this lipid transfer could be a novel strategy to control Leishmania growth.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Patrick O. Sakyi, Samuel K. Kwofie, Julius K. Tuekpe, Theresa M. Gwira, Emmanuel Broni, Whelton A. Miller, Michael D. Wilson, Richard K. Amewu
Summary: Leishmaniasis is a global public health concern and there is a need to find new leads due to resistance and lack of efficacy of current drugs. In this study, a combination of In silico and in vitro approaches was used to identify potential synthetic small-molecule inhibitors targeting LdSMT. The study identified nine compounds as potential hit molecules, which could be optimized to develop potent antileishmanial therapeutic agents.
Article
Microbiology
Damian A. Madrigal-Aguilar, Adilene Gonzalez-Silva, Blanca Rosales-Acosta, Celia Bautista-Crescencio, Jossue Ortiz-Alvarez, Carlos H. Escalante, Jaime Sanchez-Navarrete, Cesar Hernandez-Rodriguez, German Chamorro-Cevallos, Joaquin Tamariz, Lourdes Villa-Tanaca
Summary: Due to the emergence of multidrug-resistant strains of yeasts, discovering antifungal agents that target alternative molecular targets is urgently needed. This study evaluated the capacity of three different series of synthetic compounds to inhibit a specific enzyme in Candida glabrata, leading to the disruption of ergosterol synthesis and yeast viability. Two compounds, 1c and 5b, were identified as the best antifungal candidates among the compounds studied. They effectively inhibited the growth of fluconazole-resistant and fluconazole-susceptible C. glabrata strains. The compounds act by inhibiting the mevalonate synthesis pathway, resulting in a decrease in ergosterol concentration. The docking analysis suggested that their inhibitory effect may be mediated by their interaction with the enzyme's catalytic site. Further research and modifications to increase their specificity and potency are needed.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Andreza do Socorro Silva da Veiga, Fernando Tobias Silveira, Edilene Oliveira da Silva, Jose Antonio Picanco Diniz Junior, Sanderson Correa Araujo, Marliane Batista Campos, Andrey Moacir do Rosario Marinho, Geraldo Celio Brandao, Valdicley Vieira Vale, Sandro Percario, Maria Fani Dolabela
Summary: This study evaluated the morphological changes caused by fractions and subfractions obtained from barks of Aspidosperna nitidum against L. (L.) amazonensis promastigotes. The results suggest that alkaloids isolated from A. nitidum show potential as leishmanicidal agents. Scanning electron microscopy and transmission electron microscopy analysis revealed changes in the parasites' morphology after treatment with the active fractions.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Patrick Lypaczewski, Lovlesh Thakur, Aklank Jain, Sandhya Kumari, Kayla Paulini, Greg Matlashewski, Manju Jain
Summary: Leishmaniasis is a neglected tropical disease with two main types: cutaneous and visceral. Most visceral cases occur in the Indian subcontinent and East Africa due to Leishmania donovani. However, recent outbreaks of cutaneous leishmaniasis in India, such as in Himachal Pradesh, have been found to be caused by atypical phenotypes of L. donovani. Genetic analysis revealed that the strain in Himachal Pradesh is a hybrid of two independent L. donovani parasites from the ISC1 clade, found in the Nepalese highlands.
Article
Microbiology
Bart Cuypers, Pieter Meysman, Ionas Erb, Wout Bittremieux, Dirk Valkenborg, Geert Baggerman, Inge Mertens, Shyam Sundar, Basudha Khanal, Cedric Notredame, Jean-Claude Dujardin, Malgorzata A. Domagalska, Kris Laukens
Summary: Aneuploidy has a significant impact on the transcriptome, proteome, and metabolome of Leishmania donovani, leading to disruptions in stochiometric balances and metabolic differences between aneuploid strains. However, protein complex subunits and non-cytoplasmic proteins show attenuations in dosage effects. The differentially expressed proteins are enriched for those involved in protein metabolism. These findings suggest that aneuploidy in Leishmania can be adaptive and make it an attractive model for studying protein homeostasis in the context of aneuploidy.
Article
Immunology
Rahat Ali, Shams Tabrez, Sajjadul Kadir Akand, Fazlur Rahman, Atahar Husein, Mohd Arish, Ali S. Alqahtani, Mohammad Z. Ahmed, Mohammad Husain, Abdur Rub
Summary: This study evaluated the antileishmanial potential of sesamol against L. donovani, showing that sesamol inhibits parasite growth and proliferation through induction of reactive oxygen species (ROS), mitochondrial dysfunction, DNA fragmentation, cell cycle arrest, and apoptosis-like cell death. The results suggest the possible use of sesamol for the treatment of leishmaniasis following further in vivo validations.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Oncology
Antti Peltomaa, Kirsi Talala, Kimmo Taari, Teuvo L. J. Tammela, Anssi Auvinen, Teemu J. Murtola
Summary: The study found that there is an inverse association between the use of statins and cancer mortality in men with decreased cholesterol levels. This suggests that the observed reduced cancer mortality associated with statin use is related to cholesterol level. This is the first study to evaluate the separate effects of cholesterol level and statin use on cancer mortality.
Article
Immunology
Amrita Das, Mohd. Kamran, Nahid Ali
Summary: The study demonstrates that the liposomal formulation of HO-3867 is effective in inducing apoptosis in Leishmania parasites while being safe for normal murine macrophages. The treatment effectively reduces intracellular L. donovani amastigote burden by inducing parasite killing through ROS and NO activity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Aretha Chan, Jose-Mauricio Ayala, Fernando Alvarez, Ciriaco Piccirillo, George Dong, David Langlais, Martin Olivier
Summary: This study analyzed the early innate inflammatory events during L. major infection, confirming the significant role of the virulence factor GP63 in providing favorable conditions for host cell infection. The data suggest that GP63 does not greatly influence pathogen-induced inflammatory cell recruitment, but rather affects their activation status and effector function, as well as potentially influencing the internalization of promastigotes during early infection.
Article
Immunology
Jianhui Zhang, Jiao Li, Kaifeng Hu, Qi Zhou, Xiaoxiao Chen, Jinlei He, Shuangshuang Yin, Yangjian Chi, Xuechun Liao, Yuying Xiao, Hanxiao Qin, Zhiwan Zheng, Jianping Chen
Summary: This study identified differentially expressed proteins in two Leishmania donovani strains using proteomics analysis and identified potential vaccine candidates through immunoinformatics analysis. The results suggest that these candidates have the potential for intervention against visceral leishmaniasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Jairo Alfonso Mendoza-Roldan, Andrea Zatelli, Maria Stefania Latrofa, Roberta Iatta, Marcos Antonio Bezerra-Santos, Giada Annoscia, Floriana Gernone, Jan Votypka, David Modry, Lucie Ticha, Petr Volf, Domenico Otranto
Summary: This study confirms the circulation of L. infantum and L. tarentolae in sand flies, reptiles, and dogs. It also suggests that geckoes may be infected with L. infantum.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Microbiology
Karthik Ramalingam, Sonali Gangwar, Deep Chandra Balodi, Apeksha Anand, Shailendra Yadav, Subhasish Biswas, Anil Kumar Karunakaran Sasikala, Kailash Chand Gupta, Sanjay Batra, Neena Goyal
Summary: Chemotherapy is crucial for controlling visceral leishmaniasis, but current regimens have drawbacks. This study identified compound 3c as the lead candidate for treating VL, showing the best in vivo antileishmanial efficacy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Daniel P. Marcusa, Robert P. Giugliano, Jeong-Gun Park, James A. de Lemos, Christopher P. Cannon, Marc S. Sabatine
Summary: This secondary exploratory analysis evaluates the association between baseline low-density lipoprotein cholesterol and the percentage low-density lipoprotein cholesterol reduction with different pharmacotherapies. The study found that patients with lower baseline LDL-C levels had a higher percentage reduction in LDL-C with evolocumab, a more modest difference with simvastatin, and no clinically important difference with ezetimibe. These findings suggest that intensive LDL-C-lowering therapies are beneficial even for patients with relatively low baseline LDL-C levels.
Article
Biochemistry & Molecular Biology
Neelagiri Soumya, I. Sravan Kumar, S. Shivaprasad, Landage Nitin Gorakh, Neeradi Dinesh, Kayala Kambagiri Swamy, Sushma Singh
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2015)
Article
Parasitology
Neeradi Dinesh, Neelagiri Soumya, Sushma Singh
PARASITOLOGY RESEARCH
(2015)
Article
Chemistry, Medicinal
Nitin A. Lunagariya, Vikrantsinh M. Gohil, Varun Kushwah, Soumya Neelagiri, Sanyog Jain, Sushma Singh, Kamlesh K. Bhutani
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2016)
Article
Genetics & Heredity
Neelagiri Soumya, Hitendra Tandan, Mangesh V. Damre, Rahul P. Gangwal, Abhay T. Sangamwar, Sushma Singh
Article
Parasitology
Neeradi Dinesh, Soumya Neelagiri, Vinay Kumar, Sushma Singh
EXPERIMENTAL PARASITOLOGY
(2017)
Article
Immunology
Vinay Kumar, Shailendra Yadav, Neelagiri Soumya, Rohit Kumar, Neerupudi Kishore Babu, Sushma Singh
MICROBIAL PATHOGENESIS
(2017)
Article
Biochemistry & Molecular Biology
Mahesh Sharma, Naeem Shaikh, Shailendra Yadav, Sushma Singh, Prabha Garg
MOLECULAR BIOSYSTEMS
(2017)
Article
Parasitology
Neelagiri Soumya, Mitesh N. Panara, Kishore Babu Neerupudi, Sushma Singh
PARASITOLOGY INTERNATIONAL
(2017)
Article
Biochemistry & Molecular Biology
Vinay Kumar, Mahesh Sharma, Bhadoriya Ruchita Rakesh, Chandan Kumar Malik, Soumya Neelagiri, Kishore Babu Neerupudi, Prabha Garg, Sushma Singh
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Immunology
Sushma Singh, K. Raju, Deepika Jatekar, Neeradi Dinesh, M. Stanley Paul, M. E. Sobhia
MICROBIAL PATHOGENESIS
(2014)
Article
Parasitology
Sushma Singh, Neeradi Dinesh, Preet Kamal Kaur, Baigadda Shamiulla
PARASITOLOGY RESEARCH
(2014)
Article
Multidisciplinary Sciences
Preet Kamal Kaur, Neha Tripathi, Jayesh Desale, Soumya Neelagiri, Shailendra Yadav, Prasad V. Bharatam, Sushma Singh
Article
Biochemistry & Molecular Biology
Amandeep Kaur, Preet Kamal Kaur, Sushma Singh, Inder Pal Singh
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
(2014)
Article
Biochemistry & Molecular Biology
Srikant Bhagat, Parth Shah, Sanjeev K. Garg, Shweta Mishra, Preet Kamal Kaur, Sushma Singh, Asit K. Chakraborti