Review
Immunology
Albane Jouault, Alessandra Mattos Saliba, Lhousseine Touqui
Summary: This mini-review discusses how the type III secretion system (T3SS) of Pseudomonas aeruginosa and the bacterial effectors secreted through this system can activate and manipulate the host immune response, promoting the survival and establishment of the pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Shubham Chakravarty, Layla Ramos-Hegazy, Abigail Gasparovic, Gregory G. Anderson
Summary: The overexpression of polB significantly reduced T3SS transcription and repressed translation of the master T3SS regulator ExsA. Furthermore, polB does not act through previously described genetic pathways that post-transcriptionally regulate ExsA.
MICROBES AND INFECTION
(2021)
Article
Biochemistry & Molecular Biology
Charlotta Sundin, Michael Saleeb, Sara Spjut, Liena Qin, Mikael Elofsson
Summary: The study identified small molecule binders of PcrV through surface plasmon resonance screening, with subsequent analysis showing their protective effects on macrophages in a P. aeruginosa cell-based infection assay. Treatment of P. aeruginosa infections is challenging due to resistance factors and virulence systems, making virulence blocking molecules targeting PcrV valuable for development of next generation antibacterials.
Article
Microbiology
Hao Chen, Xuetao Gong, Zheng Fan, Yushan Xia, Yongxin Jin, Fang Bai, Zhihui Cheng, Xiaolei Pan, Weihui Wu
Summary: Rising antimicrobial resistance poses a severe threat to human health, and understanding bacterial regulation of virulence and antimicrobial resistance determinants is urgently needed for developing new antimicrobial strategies. This study demonstrates that a mutation in citrate synthase gltA increases the expression of type III secretion system (T3SS) genes and antibiotic tolerance in Pseudomonas aeruginosa. The stringent response is activated in the gltA mutant, and restoring the antibiotic tolerance and T3SS gene expression to wild-type level requires deletion of the (p)ppGpp synthetase gene relA. Furthermore, increased intracellular cAMP level in the gltA mutant due to the stringent response enhances the expression of the T3SS master regulator gene exsA. This work reveals the important roles of GltA in metabolism, antibiotic tolerance, and virulence, as well as a novel regulatory mechanism of the stringent response-mediated regulation of the T3SS in P. aeruginosa.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Hanh N. Lam, Tannia Lau, Adam Lentz, Jessica Sherry, Alejandro Cabrera-Cortez, Karen Hug, Annalyse Lalljie, Joanne Engel, R. Scott Lokey, Victoria Auerbuch
Summary: The development of new antibiotics is urgently needed due to the emerging threat of antibiotic-resistant bacteria. A newly discovered isomer, 4EpDN, shows potent inhibitory effects on multiple Gram-negative bacteria's injectisome type III secretion system (T3SS), possibly by preventing complete T3SS assembly.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Yushan Xia, Congjuan Xu, Dan Wang, Yuding Weng, Yongxin Jin, Fang Bai, Zhihui Cheng, Oscar P. Kuipers, Weihui Wu
Summary: YbeY is a highly conserved RNase in bacteria that plays essential roles in rRNA processing, small RNA regulation, and bacterial responses to environmental stresses. Mutation of the ybeY gene in Pseudomonas aeruginosa leads to dysregulation of secretion system gene expression and biofilm formation, indicating the pleiotropic functions of YbeY in bacterial regulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Sardar Karash, Robert Nordell, Egon A. Ozer, Timothy L. Yahr
Summary: Effector proteins translocated by the Pseudomonas aeruginosa type III secretion system play crucial roles in phagocytic avoidance and systemic spread. T3SS gene expression is common in environmental and clinical isolates, except for those from cystic fibrosis patients. A study found loss of T3SS gene expression in chronic infections beyond CF, suggesting it may be a common trait contributing to persistence.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Gertrudis Horna, Joaquim Ruiz
Summary: Pseudomonas aeruginosa is an opportunistic pathogen that mainly affects severe patients in ICUs, characterized by high levels of antibiotic resistance and a long battery of virulence factors. Among these factors, T3SS plays a key role in injecting effectors into host cells and influencing immune responses to increase bacterial survival rates. Despite extensive research on T3SS, there are still questions and further points to be clarified or established. Efforts are being made to develop T3SS-targeting molecules to improve patient outcomes.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Naybi Munoz-Cazares, Israel Castillo-Juarez, Rodolfo Garcia-Contreras, Victor Alberto Castro-Torres, Miguel Diaz-Guerrero, Jose S. Rodriguez-Zavala, Hector Quezada, Bertha Gonzalez-Pedrajo, Mariano Martinez-Vazquez
Summary: This study assessed the inhibition of quorum sensing (QS) and type III secretion systems (T3SSs) on Pseudomonas aeruginosa virulence. The synthesized halogenated furanones, named GBr, effectively reduced pyocyanin production, biofilm formation, and swarming motility, and inhibited the secretion of ExoS, ExoT, or ExoU effectors in tested strains. In a mouse model, GBr significantly decreased necrosis formation and bacterial spread more efficiently than C-30. Molecular docking analysis suggested that GBr had increased affinity for the active site of the QS LasR regulator. These findings highlight the potential of GBr as a multi-target anti-virulence therapy.
Article
Biotechnology & Applied Microbiology
Liwei Fang, Biswarup Banerjee, Xiaochen Yuan, Quan Zeng, Cuirong Liang, Xin Chen, Ching-Hong Yang
Summary: New antimicrobial therapies are urgently needed to combat antibiotic resistance. This study identified a compound, 187R, that inhibits the expression of virulence factors in Pseudomonas aeruginosa without affecting bacterial growth. The compound had limited impact on the host microbiota, suggesting that targeting virulence factors could be an alternative approach to antibiotic treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Weina Kong, Wei Luo, Yaya Wang, Yu Liu, Qianqian Tian, Cheng Zhao, Haihua Liang
Summary: This study reveals that the dual-domain GGDEF/EAL protein RmcA achieves specificity of action through physical interaction with CbrB, which extends understanding of the complex regulatory network of c-di-GMP signaling.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Cristian A. Escobar, Badreddine Douzi, Genevieve Ball, Brice Barbat, Sebastien Alphonse, Loic Quinton, Rome Voulhoux, Katrina T. Forest
Summary: Researchers have deciphered the structural integration and function of a missing component in the T2SS architecture using multiple biophysical techniques, revealing the role of XcpH in bridging different subunits. They have also provided an experimentally validated three-dimensional structural model, showcasing a complete type IV filament.
Article
Biochemistry & Molecular Biology
Jiuxin Qu, Zhao Cai, Xiangke Duan, Han Zhang, Hang Cheng, Shuhong Han, Kaiwei Yu, Zhaofang Jiang, Yingdan Zhang, Yang Liu, Fang Bai, Yingxia Liu, Lei Liu, Liang Yang
Summary: Our study suggests that SARS-CoV-2 infection can lead to genetic adaptation of bacterial pathogens, promoting chronic infections. The adapted bacteria exhibit characteristics such as excessive biofilm formation and reduced virulence, enabling them to evade immune clearance. This highlights the importance of understanding the lung microenvironment post-virus infections and superinfection mechanism in COVID-19 patients.
CELL AND BIOSCIENCE
(2022)
Article
Microbiology
Jing Wang, Yuan Luo, Yilin Gu, Hai-Lei Wei
Summary: Pseudomonas fluorescens 2P24 is a plant growth-promoting rhizobacterium with a complete SPI-1 type III secretion system gene cluster. HilA and InvF were identified as two transcriptional regulator homologs within the SPI-1 T3SS gene cluster, regulating gene expression positively and up-regulating genes, respectively. The SPI-1 mutants of 2P24 showed different phenotypes in resistance against Fusarium graminearum, biofilm formation, and chemotactic responses to glucose compared to the wild type strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Wataru Hayashi, Katsutoshi Izumi, Satoshi Yoshida, Shino Takizawa, Kanae Sakaguchi, Keita Iyori, Ken-ichi Minoshima, Shinya Takano, Maki Kitagawa, Yukiko Nagano, Noriyuki Nagano
Summary: This study investigated antimicrobial resistance trends and virulence gene prevalence among Pseudomonas aeruginosa isolates from dogs and cats in Japan. High-risk clones such as ST235 were found to be prevalent, highlighting the potential spread of these clones in companion animals.
MICROBIOLOGY SPECTRUM
(2021)
Article
Medicine, Legal
Maksymilian Wlodarski, Miron Kaliszewski, Elzbieta Anna Trafny, Malgorzata Szpakowska, Rafal Lewandowski, Aneta Bombalska, Miroslaw Kwasny, Krzysztof Kopczynski, Monika Mularczyk-Oliwa
FORENSIC SCIENCE INTERNATIONAL
(2015)
Article
Biotechnology & Applied Microbiology
ElZbieta A. Trafny, Rafal Lewandowski, Krystyna Kozlowska, Irena Zawistowska-Marciniak, Malgorzata Stepinska
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2015)
Article
Immunology
O. Olszewska-Sosinska, B. Zielnik-Jurkiewicz, M. Stepinska, M. Antos-Bielska, M. Lau-Dworak, K. Kozlowska, E. A. Trafny
PATHOGENS AND DISEASE
(2016)
Article
Biochemistry & Molecular Biology
Slawomir Lewicki, Monika Lesniak, Eugeniusz Krzysztof Machaj, Malgorzata Antos-Bielska, Elbieta Anna Trafny, Janusz Kocik, Zygmunt Pojda
JOURNAL OF LIPOSOME RESEARCH
(2017)
Article
Microbiology
M. Stepinska, O. Olszewska-Sosinska, M. Lau-Dworak, B. Zielnik-Jurkiewicz, E. A. Trafny
CURRENT MICROBIOLOGY
(2014)
Article
Microbiology
Elzbieta A. Trafny, Olga Olszewska-Sosinska, Malgorzata Antos-Bielska, Krystyna Kozlowska, Malgorzata Stepinska, Magdalena Lau-Dworak, Beata Zielnik-Jurkiewicz
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2014)
Article
Engineering, Electrical & Electronic
Yahia Achour, Jacek Starzynski, Wiktoria Kasprzycka, Elzbieta Anna Trafny
INTERNATIONAL JOURNAL OF CIRCUIT THEORY AND APPLICATIONS
(2019)
Article
Materials Science, Multidisciplinary
Paulina Natalia Osuchowska, Roman Ostrowski, Antoni Sarzynski, Marek Strzelec, Zygmunt Mierczyk, Elzbieta Anna Trafny
RESULTS IN PHYSICS
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Rafal Lewandowski, Elibieta A. Trafny, Malgorzata Stepinska, Andrzej Gietka, Pawel Kotowski, Monika Dobrzynska, Mariusz P. Lapinski
LASER TECHNOLOGY 2016: PROGRESS AND APPLICATIONS OF LASERS
(2016)
Proceedings Paper
Engineering, Electrical & Electronic
Monika Dobrzynska, Malgorzata Stepinska, Rafal Lewandowski, Andrzej Gietka, Mariusz P. Lapinski, Elzbieta A. Trafny
LASER TECHNOLOGY 2016: PROGRESS AND APPLICATIONS OF LASERS
(2016)
Proceedings Paper
Engineering, Electrical & Electronic
Paulina Natalia Osuchowska, Antoni Sarzynski, Marek Strzelec, Zdzislaw Bogdanowicz, Jan Marczak, Mariusz Piotr Lapinski, Elzbieta Anna Trafny
LASER TECHNOLOGY 2016: PROGRESS AND APPLICATIONS OF LASERS
(2016)