Review
Immunology
Alaullah Sheikh, James M. Fleckenstein
Summary: Pathogenic Escherichia coli can be classified into specific variants (pathovars) based on their behavior and the expression of specific virulence factors. These pathogens interact with CEACAMs through both core elements common to all E. coli and pathovar-specific virulence traits that target the IgV regions of CEACAMs. Recent data suggests that these interactions may not only benefit the pathogen but also provide a mechanism for pathogen elimination.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Laura Alvarez-Fraga, Minh-Duy Phan, Kelvin G. K. Goh, Nguyen Thi Khanh Nhu, Steven J. Hancock, Luke P. Allsopp, Kate M. Peters, Brian M. Forde, Leah W. Roberts, Matthew J. Sullivan, Makrina Totsika, Scott A. Beatson, Glen C. Ulett, Mark A. Schembri
Summary: This study identified the widespread presence of genes encoding Afa/Dr fimbriae in UPEC ST131 strains, and demonstrated how the integration of IS elements in the afa regulatory region affects Afa expression, altering its virulence. Evidence suggests that IS1 elements have a more widespread impact on ST131 genome evolution.
Article
Microbiology
Laura Alvarez-Fraga, Minh-Duy Phan, Kelvin G. K. Goh, Nguyen Thi Khanh Nhu, Steven J. Hancock, Luke P. Allsopp, Kate M. Peters, Brian M. Forde, Leah W. Roberts, Matthew J. Sullivan, Makrina Totsika, Scott A. Beatson, Glen C. Ulett, Mark A. Schembri
Summary: Many antibiotic resistant uropathogenic Escherichia coli (UPEC) strains belong to clones defined by their multilocus sequence type (ST), with ST131 being the most dominant. Although we have a good understanding of resistance development to fluoroquinolones and third-generation cephalosporins by ST131, our understanding of the virulence repertoire that has contributed to its global dissemination is limited. Afa/Dr fimbriae genes are found frequently in ST131 strains, and the integration of insertion sequence (IS) elements in the afa regulatory region leads to altered virulence capacity.
Article
Multidisciplinary Sciences
Nicky O'Boyle, Gillian R. Douce, Gillian Farrell, Nicholas J. W. Rattray, Mark A. Schembri, Andrew J. Roe, James P. R. Connolly
Summary: The ability of bacterial pathogens to adapt to host niches is driven by the carriage and regulation of genes. The Escherichia coli TF YhaJ exhibits distinct genome-binding dynamics and transcriptome control in different host niches. YhaJ coordinates the activation of fimbriae and the biosynthesis of amino acids.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Microbiology
Hicham Bessaiah, Carole Anamale, Jacqueline Sung, Charles M. Dozois
Summary: Pathogens are exposed to harmful conditions in the host environment, and bacterial responses play a crucial role in colonization and pathogenesis. Type 1 fimbriae in E. coli are important colonization factors and their regulation is influenced by signaling and stress. The regulation of type 1 fimbriae is potentially linked to environmental stress responses, and plays a role in regulating colonization and virulence of extraintestinal pathogenic E. coli.
Article
Agriculture, Dairy & Animal Science
Mingxu Zhou, Yang Yang, Miaomiao Wu, Fang Ma, Yue Xu, Bihua Deng, Jinqiu Zhang, Guoqiang Zhu, Yu Lu
Summary: LPF1 and LPF2 play a role in the adhesion, invasion, and biofilm formation of MPEC, with LPF2 inducing a mild TLR4-independent proinflammatory response.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jun-Young Park, Cheorl-Ho Kim, Seung-Hak Cho
Summary: In this study, we developed a FimH inhibitor to prevent adhesion of enterohemorrhagic Escherichia coli on human intestinal epithelium during the early stage of infection. By investigating the ligand binding domain of FimH and analyzing its evolutionary characteristics, we identified a potential drug target and found that glycan-like peptides can serve as initial infection inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Imed Salhi, Amal Rabti, Asma Dhehibi, Noureddine Raouafi
Summary: Bacterial diseases in livestock animals cause significant economic losses. F17A protein, a crucial virulence factor in pathogenic Escherichia coli, has been identified as a promising therapeutic target and diagnostic tool. Researchers developed anti-F17A antibodies for the detection of this protein and successfully developed a biosensor for electrochemical and fluorescent detection of purified F17A protein and live F17-positive E. coli bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Yancong Zhang, Kelsey N. Thompson, Curtis Huttenhower, Eric A. Franzosa
Summary: Researcher designed and evaluated statistical models in metatranscriptomics that incorporate different combinations of DNA and RNA normalization, finding that models adjusting transcripts relative to their encoding gene copies are significantly more accurate in identifying differential expression. Additionally, normalization of MTX measurements within species and adjusting for total species RNA balance can improve sensitivity, specificity, and interpretability of DE detection.
Article
Multidisciplinary Sciences
Sergey Peltek, Irina Meshcheryakova, Elena Kiseleva, Dmitry Oshchepkov, Alexei Rozanov, Danil Serdyukov, Evgeniy Demidov, Gennady Vasiliev, Nikolay Vinokurov, Alla Bryanskaya, Svetlana Bannikova, Vasiliy Popik, Tatyana Goryachkovskaya
Summary: Exposure of E. coli to THz radiation resulted in changes in gene activities related to cell aggregation, motility suppression, division suppression, adhesin synthesis, and cell envelope stabilization. The radiation also induced the expression of genes involved in amyloid biosynthesis. Electron microscopy revealed increased aggregation and structural aberrations in the bacterial cells, impacting cell morphology and division. Overall, THz radiation had adverse effects on bacterial populations, leading to cells with abnormal morphology.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Yuna Du, Huiqing Zhang, Yujie Guo, Kuangyu Song, Lifeng Zeng, Yiguo Chen, Zhengyu Xie, Rong Li
Summary: The study found that CD38(-/-) mice injected with Escherichia coli resulted in more severe pulmonary injuries and higher levels of cytokine mRNA expressions. CD38 deficiency increased the expressions of IL-1 beta and MCP-1 and aggravated pulmonary injury through the TLR4/ERK/NF-kB signaling pathway in sepsis.
MICROBES AND INFECTION
(2021)
Article
Chemistry, Multidisciplinary
Gregory Francius, Florian Petit, Eloise Clement, Yankel Chekli, Jean-Marc Ghigo, Christophe Beloin, Jerome F. L. Duval
Summary: Yad fimbriae are considered versatile bacterial adhesins that play a significant role in host or plant-pathogen recognition. This study used Single Molecule Force Spectroscopy to quantify the interaction forces between Yad fimbriae and self-assembled monolayers (SAM) on the nanoscale. Results showed strong adhesion of Yad fimbriae with xylose-functionalized probes, while interactions with other types of SAMs were non-specific.
Review
Biochemistry & Molecular Biology
Charles J. Dorman
Summary: This article discusses how bacteria deal with an unpredictable and often hostile environment by being unpredictable themselves, and the contributions of variable DNA topology and nucleoid-associated proteins to stochasticity in bacterial gene expression. It also describes the epigenetic effects on gene expression brought about by the modulation of topoisomerase activity in both prokaryotes and eukaryotes.
MOLECULAR MICROBIOLOGY
(2023)
Article
Immunology
Claudia A. A. Freire, Beatriz O. O. Rodrigues, Waldir P. P. Elias, Cecilia M. M. Abe
Summary: This study aimed to evaluate the presence of AAF, AFP, and CS22-related genes in EAEC strains collected from children with diarrhea. The results indicate that AAF and AFP-related genes may contribute to identify EAEC strains, while the importance of cseA as an EAEC virulence factor and genotypic marker needs further evaluation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biophysics
Tobias Dahlberg, Joseph L. Baker, Esther Bullitt, Magnus Andersson
Summary: This study investigates the biophysical function and structural interactions of adhesion pili on the surface of bacteria. Optical tweezers are used to measure the interaction dynamics of pilus subunits, and steered molecular dynamics simulations reveal atomic details of subunit interactions under tension. The results indicate that the compliance and stability of the helical filament structure are dependent on their length and the presence of the N-terminal "staple" region. This study contributes to our understanding of the essential features of adhesion pili and their importance in bacterial attachment.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Richard Gibson, Blaise Alako, Clara Amid, Ana Cerdeno-Tarraga, Iain Cleland, Neil Goodgame, Petra ten Hoopen, Suran Jayathilaka, Simon Kay, Rasko Leinonen, Xin Liu, Swapna Pallreddy, Nima Pakseresht, Jeena Rajan, Marc Rossello, Nicole Silvester, Dmitriy Smirnov, Ana Luisa Toribio, Daniel Vaughan, Vadim Zalunin, Guy Cochrane
NUCLEIC ACIDS RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Ana Luisa Toribio, Blaise Alako, Clara Amid, Ana Cerdeno-Tarraga, Laura Clarke, Iain Cleland, Susan Fairley, Richard Gibson, Neil Goodgame, Petra ten Hoopen, Suran Jayathilaka, Simon Kay, Rasko Leinonen, Xin Liu, Josue Martinez-Villacorta, Nima Pakseresht, Jeena Rajan, Kethi Reddy, Marc Rosello, Nicole Silvester, Dmitriy Smirnov, Daniel Vaughan, Vadim Zalunin, Guy Cochrane
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Nicole Silvester, Blaise Alako, Clara Amid, Ana Cerdeno-Tarraga, Laura Clarke, Iain Cleland, Peter W. Harrison, Suran Jayathilaka, Simon Kay, Thomas Keane, Rasko Leinonen, Xin Liu, Josu Martinez-Villacorta, Manuela Menchi, Kethi Reddy, Nima Pakseresht, Jeena Rajan, Marc Rossello, Dmitriy Smirnov, Ana L. Toribio, Daniel Vaughan, Vadim Zalunin, Guy Cochrane
NUCLEIC ACIDS RESEARCH
(2018)
Article
Cell & Tissue Engineering
Manolis Gialitakis, Mauro Tolaini, Ying Li, Mercedes Pardo, Lu Yu, Ana Toribio, Jyoti S. Choudhary, Kathy Niakan, Venizelos Papayannopoulos, Brigitta Stockinger
Article
Virology
Evelien M. Adriaenssens, Hans-Wolfgang Ackermann, Hany Anany, Bob Blasdel, Ian F. Connerton, David Goulding, Mansel W. Griffiths, Steven P. Hooton, Elizabeth M. Kutter, Andrew M. Kropinski, Ju-Hoon Lee, Martine Maes, Derek Pickard, Sangryeol Ryu, Zargham Sepehrizadeh, S. Sabouri Shahrbabak, Ana L. Toribio, Rob Lavigne
ARCHIVES OF VIROLOGY
(2012)
Article
Microbiology
Alice Nyambura Maina, Francis B. Mwaura, Julius Oyugi, David Goulding, Ana L. Toribio, Samuel Kariuki
CURRENT MICROBIOLOGY
(2014)
Article
Microbiology
Nicola K. Petty, Richard Bulgin, Valerie F. Crepin, Ana M. Cerdeno-Tarraga, Gunnar N. Schroeder, Michael A. Quail, Nicola Lennard, Craig Corton, Andrew Barron, Louise Clark, Ana L. Toribio, Julian Parkhill, Gordon Dougan, Gad Frankel, Nicholas R. Thomson
JOURNAL OF BACTERIOLOGY
(2010)
Article
Microbiology
Derek Pickard, Ana Luisa Toribio, Nicola K. Petty, Andries van Tonder, Lu Yu, David Goulding, Bart Barrell, Richard Rance, David Harris, Michael Wetter, John Wain, Jyoti Choudhary, Nicholas Thomson, Gordon Dougan
JOURNAL OF BACTERIOLOGY
(2010)
Article
Biochemistry & Molecular Biology
Guy Cochrane, Blaise Alako, Clara Amid, Lawrence Bower, Ana Cerdeno-Tarraga, Iain Cleland, Richard Gibson, Neil Goodgame, Mikyung Jang, Simon Kay, Rasko Leinonen, Xiu Lin, Rodrigo Lopez, Hamish McWilliam, Arnaud Oisel, Nima Pakseresht, Swapna Pallreddy, Youngmi Park, Sheila Plaister, Rajesh Radhakrishnan, Stephane Riviere, Marc Rossello, Alexander Senf, Nicole Silvester, Dmitriy Smirnov, Petra ten Hoopen, Ana Toribio, Daniel Vaughan, Vadim Zalunin
NUCLEIC ACIDS RESEARCH
(2013)
Article
Biochemistry & Molecular Biology
Nima Pakseresht, Blaise Alako, Clara Amid, Ann Cerdeno-Tarraga, Iain Cleland, Richard Gibson, Neil Goodgame, Tamer Gur, Mikyung Jang, Simon Kay, Rasko Leinonen, Weizhong Li, Xin Liu, Rodrigo Lopez, Hamish McWilliam, Arnaud Oisel, Swapna Pallreddy, Sheila Plaister, Rajesh Radhakrishnan, Stephane Riviere, Marc Rossello, Alexander Senf, Nicole Silvester, Dmitriy Smirnov, Silvano Squizzato, Petra ten Hoopen, Ana Luisa Toribio, Daniel Vaughan, Vadim Zalunin, Guy Cochrane
NUCLEIC ACIDS RESEARCH
(2014)
Article
Biochemistry & Molecular Biology
Nicole Silvester, Blaise Alako, Clara Amid, Ana Cerdeno-Tarraga, Iain Cleland, Richard Gibson, Neil Goodgame, Petra ten Hoopen, Simon Kay, Rasko Leinonen, Weizhong Li, Xin Liu, Rodrigo Lopez, Nima Pakseresht, Swapna Pallreddy, Sheila Plaister, Rajesh Radhakrishnan, Marc Rossello, Alexander Senf, Dmitriy Smirnov, Ana Luisa Toribio, Daniel Vaughan, Vadim Zalunin, Guy Cochrane
NUCLEIC ACIDS RESEARCH
(2015)
Article
Multidisciplinary Sciences
Wei Wang, Christine Hale, Dave Goulding, Stuart M. Haslam, Berangere Tissot, Christopher Lindsay, Stephen Michell, Rick Titball, Jun Yu, Ana Luisa Toribio, Raffaella Rossi, Anne Dell, Allan Bradley, Gordon Dougan
Article
Virology
Elizabeth M. Kutter, Kyobi Skutt-Kakaria, Bob Blasdel, Ayman El-Shibiny, Anna Castano, Daniel Bryan, Andrew M. Kropinski, Andre Villegas, Hans-Wolfgang Ackermann, Ana L. Toribio, Derek Pickard, Hany Anany, Todd Callaway, Andrew D. Brabban
Article
Microbiology
Nicola K. Petty, Theresa Feltwell, Derek Pickard, Simon Clare, Ana L. Toribio, Maria Fookes, Kevin Roberts, Rita Monson, Satheesh Nair, Robert A. Kingsley, Richard Bulgin, Siouxsie Wiles, David Goulding, Thomas Keane, Craig Corton, Nicola Lennard, David Harris, David Willey, Richard Rance, Lu Yu, Jyoti S. Choudhary, Carol Churcher, Michael A. Quail, Julian Parkhill, Gad Frankel, Gordon Dougan, George P. C. Salmond, Nicholas R. Thomson
Article
Microbiology
Ana Luisa Toribio, Derek Pickard, Ana M. Cerdeno-Tarraga, Nicola K. Petty, Nicholas Thomson, George Salmond, Gordon Dougan
GENOME ANNOUNCEMENTS
(2014)