Article
Biochemistry & Molecular Biology
Pernille Sos Hovgaard Jensen, Maja Johansen, Lasse K. Bak, Lars Juhl Jensen, Christina Kjaer
Summary: Study finds that gene expression analysis may be influenced by pre-analytical factors, but the collection time, tissue size, collection method, and RNA isolation method do not significantly affect the yield and integrity of brain-derived RNA, with potential practical and financial implications for clinical genomic departments and other laboratory settings.
NEUROCHEMICAL RESEARCH
(2021)
Article
Oncology
Ana Dolinar, Gasper Grubelnik, Irena Srebotnik-Kirbis, Margareta Strojan Flezar, Margareta Zlajpah
Summary: The extraction of nucleic acids using spin columns provides the highest quantity and quality of nucleic acids. The isolation of nucleic acids is feasible under different storage conditions, but higher temperature and longer storage duration are associated with a decline in the quality of isolated nucleic acids. Successful molecular testing requires a minimum number of cells and can detect specific mutations even at a low mutated cell percentage.
Article
Medical Laboratory Technology
Pieter Vermeersch, Glynis Frans, Alexander von Meyer, Sean Costelloe, Giuseppe Lippi, Ana-Maria Simundic
Summary: ISO 15189:2012 standard aims to improve quality in medical laboratories, including the pre-analytical phase. However, there are significant differences in how technical auditors interpret the pre-analytical requirements. EFLM WG-PRE provides guidance for laboratories on meeting these requirements.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Review
Chemistry, Analytical
Francesca Merlo, Antonella Profumo, Andrea Speltini
Summary: Steroid hormones, widely used in medicine, are also known as endocrine-disrupting chemicals due to their presence in water bodies. Various sample treatment protocols, particularly liquid- and solid-phase extractions, have been developed for their sensitive determination. Recent methods have simplified protocols and improved sample throughput, with further potential for overall impact. The AGREEprep tool highlights the strengths and weaknesses of selected procedures and assesses their environmental impact based on green sample preparation principles.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Christina Vakh, Marek Tobiszewski
Summary: Food of animal origin is important for a healthy and sustainable diet, but residues of pharmaceuticals, particularly antibiotics, can cause health problems. Analytical methods used to detect contaminants in food need to be simple, selective, high throughput, and have low detection limits. Recently, greenness metric tools, such as AGREE, have been used to evaluate the environmental impact of analytical procedures. AGREE was used to assess the greenness of liquid and solid-phase extraction procedures for detecting antibiotic residues in food samples. The procedures were compared based on twelve input variables, and the total score indicated the overall impact on greenness improvement.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Henrik Hjelmgren, Emelie Heintz, Britt-Marie Ygge, Nina Andersson, Bjorn Nordlund
Summary: This study estimated the average direct cost of pre-analytical error (PAE) in pediatric care, specifically in blood drawings, per year and per 10,000 procedures. The study found that PAEs lead to significant increases in costs in pediatric hospital care.
Article
Environmental Sciences
Jeff Wagner, William Robberson, Harry Allen
Summary: This study demonstrates how the precision of microplastic (MP) analyses can be assessed in a laboratory setting. The results show that the MP methods used in different laboratories generally yield repeatable results. The precision estimates obtained from the analyses can be used to ensure the quality of MP results and compare them to other data. Additionally, re-analyses of reference samples can be used to assess and compare precision between laboratories.
Article
Chemistry, Analytical
Menyar Ben Jaballah, Abdelhafid Karrat, Aziz Amine, Cherif Dridi
Summary: A novel approach using a smartphone for the detection of Cr (VI) has been developed through two different platforms. The first platform, DPC-CS-PAD, utilizes a crosslinking reaction of chitosan with DPC to create a paper-based analytical device. The second platform, DPC-Nylon-PAD, involves the covalent immobilization of DPC onto a Nylon paper. Both platforms show good detection performance for Cr (VI) within certain concentration ranges.
Article
Medical Laboratory Technology
Pinar Eker
Summary: This study aimed to estimate the direct financial loss created by contaminated urine sampling and highlighted cost elements to potentially contribute to prioritization in corrective and preventive actions. It is the first cost study to provide pricing for human resources, material, waste cost, and the amount reimbursed by SSI per rejection sample. The findings may lead to reduced antibiotic resistance and inspire new additions to detailed pre-analytical error cost studies.
CLINICAL LABORATORY
(2022)
Article
Medical Laboratory Technology
Chao Zhang, Ying Yan, Jie Zeng, Jiali Liu, Na Dong, Chuanbao Zhang
Summary: The National Centre for Clinical Laboratories (NCCL) in China launched the EQA Program for blood lead measurement in 2006 to assess its standardisation process. The results showed that the pass rates of participating laboratories gradually increased over six years, while the inter-laboratory mean CVs decreased. Graphite Furnace Atomic Absorption Spectrometry (GFAAS) and Tungsten Ship Atomic Absorption Spectrometry (TSAAS) demonstrated better performances compared to other detection methods.
CLINICA CHIMICA ACTA
(2023)
Article
Medical Laboratory Technology
Wim Coucke, Corinne Charlier, Kathleen Croes, Boris Mahieu, Hugo Neels, Christophe Stove, Jan Tytgat, Andre Vanescote, Alain G. Verstraete, Sarah Wille, Arnaud Capron
Summary: This study evaluates the performance of automated systems in ethanol concentration analysis. The results show that Alinity c performs poorly in terms of bias and measurement uncertainty, while Architect and Cobas c501/c502 systems have the lowest variability.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Mohammed F. F. Alkadhem, Lucie M. F. Wagenmakers-Huizenga, Marjan Wouthuyzen-Bakker, Anneke C. C. Muller Kobold
Summary: This study investigates the stability of synovial calprotectin and its susceptibility to potential interfering factors. It is found that factors such as time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination do not significantly affect the analysis of synovial calprotectin.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Hematology
Silmara Aparecida de Lima Montalvao, Anna Lowe, Steve Kitchen
Summary: This article explores the benefits of external quality assessment (EQA) programs, including improving local laboratory performance, identifying inadequate diagnostic tests, and evaluating new technologies in the field of blood coagulation. The study emphasizes the educational role of EQA programs in helping countries manage their public policy and in addressing the need for new technologies in haemostasis on an international level.
Review
Medical Laboratory Technology
Elena Revuelta-Lopez, Jaume Barallat, Adriana Cserkoova, Carolina Galvez-Monton, Allan S. Jaffe, James L. Januzzi, Antoni Bayes-Genis
Summary: Clinical biomarker research in the cardiovascular field is rapidly growing, with a multidimensional approach using different biomarkers. However, pre-analytical variables need to be considered to avoid errors that may compromise research results. Adequate study design and basic considerations for biospecimen management are essential for providing efficient and valid data.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Genetics & Heredity
Yuanhang Liu, Aditya Bhagwate, Stacey J. Winham, Melissa T. Stephens, Brent W. Harker, Samantha J. McDonough, Melody L. Stallings-Mann, Ethan P. Heinzen, Robert A. Vierkant, Tanya L. Hoskin, Marlene H. Frost, Jodi M. Carter, Michael E. Pfrender, Laurie Littlepage, Derek C. Radisky, Julie M. Cunningham, Amy C. Degnim, Chen Wang
Summary: Failed bioinformatics qc in FFPE samples was associated with low sample-wise correlation, reads mapped to gene regions, and detectable genes. A decision tree model based on RNA concentration and library qubit values achieved a high F score in predicting QC status of FFPE samples. Recommendations include a minimum RNA concentration of 25 ng/ul and pre-capture library output of 1.7 ng/ul for adequate RNA-seq data.
BMC MEDICAL GENOMICS
(2022)
Article
Oncology
Laura Sala, Stefano Bonomi, Alessandra Fabbri, Chiara Maura Ciniselli, Annalisa Bardelli, Paolo Verderio, Giancarlo Pruneri, Umberto Cortinovis
Summary: This study investigated the use of the PEAK PlasmaBlade and acellular dermal matrix Veritas in implant-based breast reconstruction in the setting of radiotherapy. The results showed that this approach could lead to lower complication rates and higher levels of patient and surgeon satisfaction.
Article
Biology
Yoichiro Shibuya, Akishige Hokugo, Hiroko Okawa, Takeru Kondo, Daniel Khalil, Lixin Wang, Yvonne Roca, Adam Clements, Hodaka Sasaki, Ella Berry, Ichiro Nishimura, Reza Jarrahy
Summary: Attempts to minimize scarring after surgery remain challenging for surgeons. This study identified a compound (Dwn1) that suppressed the expression of Npas2, a protein involved in wound healing. Treatment with Dwn1 resulted in faster and stronger healing of dermal wounds in mice, with reduced scarring.
Review
Immunology
Chiara Maura Ciniselli, Mara Lecchi, Mariangela Figini, Cecilia C. Melani, Maria Grazia Daidone, Daniele Morelli, Emanuela Zito, Giovanni Apolone, Paolo Verderio
Summary: Despite the global public health emergency of the COVID-19 pandemic, studies show that healthcare workers in Italy exhibit a robust antibody response after receiving the second vaccine dose. In a longitudinal study, 99.81% of HCWs developed an antibody response one month after the second dose, and six months later, all HCWs still showed positivity to antibodies, although at lower levels. The literature review and institutional study both confirm a strong antibody response in HCWs even seven months after completing the cycle of vaccination doses.
Article
Biochemistry & Molecular Biology
Valentina Doldi, Mara Lecchi, Silva Ljevar, Maurizio Colecchia, Elisa Campi, Giovanni Centonze, Cristina Marenghi, Tiziana Rancati, Rosalba Miceli, Paolo Verderio, Riccardo Valdagni, Paolo Gandellini, Nadia Zaffaroni
Summary: This study investigated the potential of periostin as a stromal biomarker in prostate cancer (PCa) prostatectomies. It was found that periostin expression in the peritumoral stroma correlates with PCa grade and aggressive disease features. Additionally, the integration of periostin and sparc circulating levels improves the prediction of disease reclassification in low-risk PCa patients enrolled in active surveillance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Oncology
Gaia Colletti, Chiara Maura Ciniselli, Emanuele Rausa, Stefano Signoroni, Ivana Maria Francesca Cocco, Andrea Magarotto, Maria Teresa Ricci, Clorinda Brignola, Andrea Mancini, Federica Cavalcoli, Laura Cattaneo, Massimo Milione, Paolo Verderio, Marco Vitellaro
Editorial Material
Agriculture, Dairy & Animal Science
Paolo Verderio, Mara Lecchi, Chiara Maura Ciniselli, Bjorn Shishmani, Giovanni Apolone, Giacomo Manenti
Summary: The 3Rs principle, consisting of Replacement, Reduction and Refinement, aims to promote ethical and scientifically valid animal experimentation. It requires the consideration of alternative models, statistical designs, and improvements in experimental conditions. The principle was first introduced in a document by the Council Directive in 1986 and developed further in the European Parliament and Council Directive in 2010. This article emphasizes the importance of the 3Rs principle, particularly the concept of Reduction, and highlights the need for its consistent implementation among countries and research teams.
Article
Orthopedics
Bernhard Springer, Robin Dreisbach, Klaus-Dieter Schatz, Bernd Kubista, Wenzel Waldstein
Summary: This study evaluated the information quality of YouTube videos regarding postoperative patient information and rehabilitation after arthroscopic rotator cuff repair. Most of the videos were of poor quality, with videos uploaded by medically trained professionals offering better information. The findings suggest that physicians should provide alternative sources of high-quality information to patients.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2023)
Article
Gastroenterology & Hepatology
E. Rausa, G. Colletti, C. M. Ciniselli, S. Signoroni, V. Duroni, F. Cavalcoli, A. Magarotto, M. T. Ricci, C. Brignola, D. Biasoni, P. Verderio, M. Vitellaro
Summary: This study investigated the effectiveness of superior rectal artery (SRA) preservation in reducing anastomotic leakage during total colectomy with ileorectal anastomosis (TC/IRA) in patients with familial adenomatous polyposis (FAP). The results showed that SRA preservation was associated with fewer complications, lower anastomotic leakage events, and lower overall surgical complications and pelvic sepsis rates. However, it increased operative time while reducing hospital stay.
TECHNIQUES IN COLOPROCTOLOGY
(2023)
Article
Multidisciplinary Sciences
Kieran D. O'Malley, Henry Schofield, Patrick G. R. Wright, Daniel Hargreaves, Tom Kitching, Marina Bollo Palacios, Fiona Mathews
Summary: Identifying peaks of bat activity through acoustic monitoring can help locate bat colonies, and a triage system based on this method can be used to prioritize survey efforts. This study successfully developed a methodology for predicting woodland occupancy by barbastelle bats using acoustic data, and validated it through citizen science surveys and specialist tracking surveys.
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Article
Gastroenterology & Hepatology
Emanuele Rausa, Chiara Maura Ciniselli, Stefano Signoroni, Laura Sophie Boer, Lois Oldhoff, Laura Dijk, Franka Van Luijk, Maria Teresa Ricci, Chiara Ghidoli, Valeria Duroni, Clorinda Brignola, Claudia Borreani, Sara Alfieri, Giovanni Apolone, Paolo Verderio, Marco Vitellaro
Summary: This study investigated the quality of life of colorectal adenomatous polyposis patients following prophylactic surgery and found that most patients adapt to changes in bowel functioning, but a minority may face social and sexual issues.
INTERNATIONAL JOURNAL OF COLORECTAL DISEASE
(2023)
Article
Mycology
Relber Aguiar Goncales, Igor Godinho Portis, Thaila Fernanda dos Reis, Luiz Roberto Basso Junior, Roberto Martinez, Heng Zhu, Maristela Pereira, Celia Maria de Almeida Soares, Paulo Sergio Rodrigues Coelho
Summary: In this study, expression analysis of GPI-anchored proteins in Paracoccidioides brasiliensis was conducted. The findings revealed increased expression levels of certain proteins in the mycelium and yeast forms, and these proteins showed immune reactivity against sera obtained from PCM patients. These results provide valuable information about the function of GPI-anchored proteins and their potential as diagnostic markers.
FRONTIERS IN FUNGAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Susanna Zanutto, Chiara Maura Ciniselli, Antonino Belfiore, Valentina Dall'Olio, Laura Tizzoni, Luca Varinelli, Marco Alessandro Pierotti, Luigi Battaglia, Paolo Verderio, Marcello Guaglio, Manuela Gariboldi
Summary: Although miRNA signatures were not predictive of colorectal cancer recurrence, a decrease in expression of miRNAs from the CL signature was observed 1 year after surgery, while miR-335-5p from the HgA signature was higher in AWD patients before surgery. These findings suggest the potential use of miRNAs as non-invasive biomarkers for monitoring patients at risk of relapse.
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS
(2022)
Article
Biochemical Research Methods
K. Ramki, G. Thiruppathi, Selva Kumar Ramasamy, P. Sundararaj, P. Sakthivel
Summary: A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semiaqueous solution. The probe exhibited enhanced fluorescence in its aggregated state and even higher fluorescence when chelated with Hg(II). The probe demonstrated high sensitivity and specificity for Hg(II) detection and was successfully applied for imaging Hg(II) in a living model.
Article
Biochemical Research Methods
Qun Zhang, Rui Yang, Gang Liu, Shiyan Jiang, Jiarui Wang, Juqiang Lin, Tingyin Wang, Jing Wang, Zufang Huang
Summary: This research aims to develop a cost-effective and portable method for measuring creatinine levels using the enhanced Tyndall effect phenomenon. The method offers a promising solution for monitoring renal healthcare in resource-limited settings.