Article
Nutrition & Dietetics
Sufang Fan, Junmei Ma, Chunsheng Li, Yanbo Wang, Wen Zeng, Qiang Li, Jinru Zhou, Liming Wang, Yi Wang, Yan Zhang
Summary: A UPLC-MS/MS method was developed for rapid screening and quantitative analysis of tropomyosin (TM) in shrimp and crab. This method involved the use of immunoaffinity column, trypsin digestion, Easy-nLC 1000-Q Exactive separation, and analysis of spectrums using Thermo Proteome Discoverer 1.4 software. The method showed good linearity and low limit of quantification, providing technical support for self-testing by food manufacturers and improvement of allergen labeling in China.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemical Research Methods
David M. Hollenstein, Margarita Maurer-Granofszky, Wolfgang Reiter, Dorothea Anrather, Thomas Gossenreiter, Riccardo Babic, Natascha Hartl, Claudine Kraft, Markus Hartl
Summary: We present an effective method to reduce codigestion of bead-bound ligands in affinity purification-mass spectrometry experiments. The method utilizes nontoxic chemicals and mild chemical reaction conditions, leading to a significant reduction in contaminating ligand peptides while improving sensitivity and quantitative accuracy in analysis.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Lingyu Wang, Liqi Xie, Zhongli Zhang
Summary: Chemical crosslinking followed by mass spectrometric identification can provide valuable structural information on the molecular binding interface. This study developed and validated a workflow using two different linkers for the determination of antigen domains targeted by therapeutic antibodies. The results showed the importance of zero-length crosslinks and demonstrated the capacity of XL/MS in protein interaction analysis.
JOURNAL OF PROTEOMICS
(2023)
Article
Engineering, Environmental
Yufeng Gong, Diwen Yang, Holly Barrett, Jianxian Sun, Hui Peng
Summary: Advancements in nontargeted analysis have allowed for the detection of multiple chemical contaminants. However, environmental toxicology approaches are lacking, impeding the transition to health risk assessment. A protein-guided approach called APNA has been developed to screen bioactive chemical contaminants at the exposome-wide level. This approach utilizes a tagged functional protein to isolate and identify contaminants, leading to the discovery of new ligands and protein targets. A proposed framework, eCPIN, aims to establish a comprehensive map of interactions between chemical contaminants and human proteins to facilitate screening and identification at a proteome-wide level.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anusha Valpadashi, Sylvie Callegari, Andreas Linden, Piotr Neumann, Ralf Ficner, Henning Urlaub, Markus Deckers, Peter Rehling
Summary: In this study, a crosslinking-mass spectrometry approach was used to determine the molecular arrangement of subunits of the human TIM22 complex, revealing unexpected features and opening up new avenues for a deeper investigation into the steps required for TIM22-mediated translocation in humans.
Article
Chemistry, Analytical
Daniel Petras, Andres Mauricio Caraballo-Rodriguez, Alan K. Jarmusch, Carlos Molina-Santiago, Julia M. Gauglitz, Emily C. Gentry, Pedro Belda-Ferre, Diego Romero, Shirley M. Tsunoda, Pieter C. Dorrestein, Mingxun Wang
Summary: ChemProp is a tool for contextualizing molecular networks by scoring abundance changes between connected nodes over sequential data series, which can help prioritize potential biological and chemical transformations or proportional changes of related compounds. This workflow has been tested on defined mixtures and shown utility in prioritizing specific molecules within biological samples, including bacterial transformations of bile acids, human drug metabolism, and bacterial natural products biosynthesis. The ChemProp workflow is freely available through the Global Natural Products Social Molecular Networking (GNPS) environment.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Tyler L. Peterson, Gabe Nagy
Summary: The new high-throughput screening method based on cIMS-MS separation allows rapid resolution of alpha/beta anomers for carbohydrates and detection of their impurities at levels below 2%, demonstrating high-throughput nature with experiments completed in 1 minute. This methodology can also be extended to separate isomeric mixtures of protected disaccharides, illustrating its utility beyond monosaccharides.
Article
Chemistry, Analytical
Zdravko Siketic, Iva Bogdanovic Radovic, Marko Barac, Marko Brajkovic, Marijana Popovic Hadzija
Summary: MeV SIMS is a type of secondary ion mass spectrometry (SIMS) technique that uses MeV energy ions to desorb molecules from a sample surface. This study presents a new system for molecular imaging of organic materials using a continuous analytical beam and a start trigger based on the detection of secondary electrons. The system allows for the imaging of samples with a lateral resolution of about 20 μm and the analysis of samples of any thickness. The imaging capabilities of MeV SIMS are demonstrated on various samples to show its potential applications in biology and forensic science.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Adway O. Zacharias, Zixiang Fang, Aurchie Rahman, Akash Talukder, Sharel Cornelius, Saiful M. Chowdhury
Summary: Protein post-translational modifications and interactions are key research areas in mass spectrometry-based proteomics, but selective enrichment of modifications/interactions of interest during sample preparation remains a major bottleneck. Various strategies have been developed to target specific modifications/interactions based on physical or chemical properties, with only a few successful applications for systematic proteome-wide study.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Food Science & Technology
Sandrine Livet, Sylvia Worbs, Herve Volland, Stephanie Simon, Martin B. Dorner, Francois Fenaille, Brigitte G. Dorner, Francois Becher
Summary: The study developed a MALDI-TOF mass spectrometry approach for fast and sensitive detection of abrin in food samples, providing a new method for analyzing the toxin.
Article
Chemistry, Analytical
Sadjad Fakouri Baygi, Yashwant Kumar, Dinesh Kumar Barupal
Summary: The poor chemical annotation of high-resolution mass spectrometry data hinders the use of untargeted metabolomics datasets. To address this issue, we developed a new software called IDSL.CSA, which enables the chemical annotation of high-resolution mass spectrometry coupled with liquid chromatography peaks even without MS2 fragmentation spectra. Validation tests showed comparable annotation rates using IDSL.CSA libraries and MS/MS libraries for commonly detected endogenous metabolites in human blood samples. The IDSL.CSA package can be used for creating and searching composite spectra libraries from any untargeted metabolomics dataset generated using high-resolution mass spectrometry coupled to liquid or gas chromatography instruments.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Richard Marins da Silva, Valeria Monteze Guimaraes, Lizzy Ayra Alcantara Verissimo, Marcia Cristina Teixeira Ribeiro Vidigal, Valeria Paula Rodrigues Minim, Luis Antonio Minim
Summary: This study successfully extracted and purified gamma-conglutin, a protein from lupin seeds known for its blood glucose lowering properties. The purification process was effective in obtaining a high degree of purity, and the protocol used was simple and easily scalable.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Oncology
Francesca Piaggio, Michela Croce, Francesco Reggiani, Paola Monti, Cinzia Bernardi, Marianna Ambrosio, Barbara Banelli, Mehmet Dogrusoez, Ralf Jockers, Domenico Bordo, Roberto Puzone, Silvia Viaggi, Domenico Coviello, Francesco B. Lanza, Martina Bartolucci, Andrea Petretto, Carlo Mosci, Rosaria Gangemi, Pieter A. van der Velden, Martine J. Jager, Ulrich Pfeffer, Adriana Amaro
Summary: This study investigated the impact of GNAQ and GNA11 gene mutations on the characteristics and prognosis of uveal melanoma. The results showed that GNA11 mutation was associated with worse prognosis and high-risk cytogenetic, mutational, and molecular tumor characteristics. Additionally, G-proteins encoded by GNAQ and GNA11 had different protein interaction partners, and differential DNA methylation might contribute to different progression risks.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Chemistry, Analytical
Max K. Edney, Anna M. Kotowska, Matteo Spanu, Gustavo F. Trindade, Edward Wilmot, Jacqueline Reid, Jim Barker, Jonathan W. Aylott, Alexander G. Shard, Morgan R. Alexander, Colin E. Snape, David J. Scurr
Summary: Modern mass spectrometry techniques produce a wealth of spectral data, but the volume and complexity of data can hinder interpretation. This study applies molecular formula prediction to interpret 3D OrbiSIMS data and achieves successful results on different samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shaunak Raval, Pauline Douglas, Danny Laurent, Morgan F. Khan, Susan P. Lees-Miller, David C. Schriemer
Summary: Affinity-purification mass spectrometry (AP-MS) is a technique used to identify protein-protein interactions. Traditional methods suffer from non-specific binding, but the use of microbead technology improves detection efficiency and suppresses non-specific binding. Experimental results show that saturated solid-phase support and a small amount of beads are sufficient to generate comprehensive protein interaction maps.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
K. Ramki, G. Thiruppathi, Selva Kumar Ramasamy, P. Sundararaj, P. Sakthivel
Summary: A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semiaqueous solution. The probe exhibited enhanced fluorescence in its aggregated state and even higher fluorescence when chelated with Hg(II). The probe demonstrated high sensitivity and specificity for Hg(II) detection and was successfully applied for imaging Hg(II) in a living model.
Article
Biochemical Research Methods
Qun Zhang, Rui Yang, Gang Liu, Shiyan Jiang, Jiarui Wang, Juqiang Lin, Tingyin Wang, Jing Wang, Zufang Huang
Summary: This research aims to develop a cost-effective and portable method for measuring creatinine levels using the enhanced Tyndall effect phenomenon. The method offers a promising solution for monitoring renal healthcare in resource-limited settings.