Review
Pharmacology & Pharmacy
Mark L. Dallas, Damian Bell
Summary: It is clear that APC technology has significantly advanced drug discovery programs, particularly in the fields of neuroscience and cardiovascular research. The challenge for the future lies in keeping up with fundamental research and improving the translation of the large datasets obtained.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Chemistry, Multidisciplinary
Ke Yang, Ke Ma, Manman Yang, Yinghua Lv, Yuxin Pei, Zhichao Pei
Summary: A supramolecular nanoprodrug (DOX@GP5 Pro-NFA) was developed, which could target tumor cells and release DOX/NFA responsively. The nanoprodrug showed the ability to overcome drug resistance through inhibiting chloride channels and inhibiting cell migration. This strategy can enhance chemotherapy potency through reverse chemoresistance.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Jing Gong, Yu Gong, Ting Zou, Yuxiao Zeng, Cao Yang, Lingyue Mo, Jiahui Kang, Xiaotang Fan, Haiwei Xu, Jun Yang
Summary: A controllable perfusion microfluidic chip (CPMC) was designed to culture retinal organoids (ROs) derived from human pluripotent stem cells. The results demonstrated that this system allowed for expansion of retinal progenitor cell (RPC) pool, orchestration of retinal ganglion cell (RGC) specification, and axon growth. This study highlights the advantages of the CPMC in promoting RO growth and providing a controllable platform for the maturity of RGCs, with promising applications in modeling RGC-related disorders, drug screening, and cell transplantation.
Review
Biochemistry & Molecular Biology
Christophe Simard, Margaux Aize, Sebastien Chaigne, Harlyne Mpweme Bangando, Romain Guinamard
Summary: The role of ion channels in cardiac activity, morphological remodeling, and valve development and remodeling is discussed. Mutation in ion channel genes is observed in patients with malformation, such as bicuspid aortic valve. Ion channels are also involved in the morphological remodeling of valves, leading to aortic stenosis. Understanding the role of ion channels in the progression of aortic stenosis is crucial for developing new therapeutic approaches to avoid valve replacement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Zeyu Deng, Vipin Kumar, Felix T. Bolle, Fernando Caro, Alejandro A. Franco, Ivano E. Castelli, Pieremanuele Canepa, Zhi Wei Seh
Summary: Battery research often relies on trial-and-error, but understanding of battery interfaces is lacking. To transform research methods, theory and computation can be used together to guide targeted experiments. Automated and intelligent computational tools have been developed to accelerate material description.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Article
Chemistry, Physical
Nur Sofina Mohamad Johari, Antranik Jonderian, Shipeng Jia, Victor Cozea, Elissa Yao, Syed Bahari Ramadzan Syed Adnan, Noraini Ahmad, Eric McCalla
Summary: This study develops a hybrid solid-state electrolyte (HybSSE) made of halloysite clay-derived Na2ZnSiO4 ceramic and ionic liquid. The results show a significantly improved ionic conductivity, making it a feasible option for quasi-solid sodium-ion batteries.
JOURNAL OF POWER SOURCES
(2022)
Article
Multidisciplinary Sciences
Zhuo-Xing Shi, Zhi-Chao Chen, Jia-Yong Zhong, Kun-Hua Hu, Ying-Feng Zheng, Ying Chen, Shang-Qian Xie, Xiao-Chen Bo, Feng Luo, Chong Tang, Chuan-Le Xiao, Yi-Zhi Liu
Summary: Long-read single-cell RNA isoform sequencing can reveal the complex landscape of alternative RNA splicing in individual cells, but it has a low read throughput. The authors developed circular consensus sequencing methods to achieve high-throughput and high-accuracy single-cell RNA isoform sequencing.
NATURE COMMUNICATIONS
(2023)
Article
Anesthesiology
Reiko Horishita, Yuichi Ogata, Ryo Fukui, Ryo Yamazaki, Kuniaki Moriwaki, Susumu Ueno, Nobuyuki Yanagihara, Yasuhito Uezono, Yuka Yokoyama, Kouichiro Minami, Takafumi Horishita
Summary: This study investigated the effects of local anesthetics on the 2-aminoethoxydiphenyl borate (2APB)-induced currents of the TRPV3 channel expressed in Xenopus oocytes. The results showed that clinically used local anesthetics inhibited the 2APB-activated currents from the TRPV3 channel in a concentration-dependent manner at pharmacologically relevant concentrations, possibly through extracellular interactions with the channel pore.
ANESTHESIA AND ANALGESIA
(2021)
Article
Cell Biology
Tobias Ruck, Stefanie Bock, Steffen Pfeuffer, Christina B. Schroeter, Derya Cengiz, Paul Marciniak, Maren Lindner, Alexander Herrmann, Marie Liebmann, Stjepana Kovac, Lukas Gola, Leoni Rolfes, Marc Pawlitzki, Nils Opel, Tim Hahn, Udo Dannlowski, Thomas Pap, Felix Luessi, Julian A. Schreiber, Bernhard Wuensch, Tanja Kuhlmann, Guiscard Seebohm, Bjoern Tackenberg, Patricia Seja, Frank Doering, Erhard Wischmeyer, Achmet Imam Chasan, Johannes Roth, Luisa Klotz, Gerd Meyer zu Hoerste, Heinz Wiendl, Tobias Marschall, Stefan Floess, Jochen Huehn, Thomas Budde, Tobias Bopp, Stefan Bittner, Sven G. Meuth
Summary: This study reveals how K(2P)18.1 translates TCR signals into thymic T cell fate decisions and Treg development, as well as its role in human Treg development. The findings provide a basis for therapeutic utilization of Treg in several human disorders.
Article
Instruments & Instrumentation
Michael Moorehead, Benoit Queylat, Hongliang Zhang, Kim Kriewaldt, Adrien Couet
Summary: Ion irradiation has been used as a substitute for neutron irradiation experiments due to their faster speed and lower cost, but ion irradiation facilities need to upgrade to accommodate new material platforms. The University of Wisconsin-Madison developed a high-throughput ion irradiation system that can handle a large number of samples and uses infrared laser heating to achieve high-temperature, high-throughput irradiation.
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
(2021)
Review
Biochemistry & Molecular Biology
Wei Xiang, Yi Hui Lam, Giridharan Periyasamy, Charles Chuah
Summary: Acute myeloid leukemia (AML) is a complex hematological malignancy with extensive heterogeneity, making it a prototype for personalized medicine. Recent studies have shown the potential of implementing genomic and phenotypic screens to improve survival in refractory AML patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Delphine Ji, Zheng-wei Luo, Andrea Ovcjak, Rahmah Alanazi, Mei-Hua Bao, Zhong-Ping Feng, Hong-Shuo Sun
Summary: Ion channels, especially TRPM2, play important roles in brain tumours, particularly in relation to oxidative stress and inflammation. Investigating TRPM2 as a potential therapeutic target for brain tumours is of significant interest in the fields of ion channels and pharmacology.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Biochemistry & Molecular Biology
Alexander L. R. Lubbock, Leonard A. Harris, Vito Quaranta, Darren R. Tyson, Carlos F. Lopez
Summary: Thunor is an open-source software platform designed to manage, analyze, and visualize large dose-dependent cell proliferation datasets. It supports both end-point and time-based proliferation assays, providing a user-friendly interface with interactive plots.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yuan Shui, Zhao-Wen Wang
Summary: Heterologous expression of connexins and innexins in Xenopus oocytes is a powerful approach for studying the biophysical properties of gap junctions. However, it is technically challenging and requires technical modifications to make it more convenient and reliable.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Andre Bazzone, Maria Barthmes, Cecilia George, Nina Brinkwirth, Rocco Zerlotti, Valentin Prinz, Kim Cole, Soren Friis, Alexander Dickson, Simon Rice, Jongwon Lim, May Fern Toh, Milad Mohammadi, Davide Pau, David J. Stone, John J. Renger, Niels Fertig
Summary: This study investigated the function of TMEM175 channel in lysosomes using solid supported membrane-based electrophysiology (SSME). The results showed that TMEM175 is a pH-dependent ion channel, and decreasing cytosolic pH inhibits its function. Additionally, the effects of different compounds on TMEM175 were evaluated and the advantages and disadvantages of different experimental techniques were compared.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
K. Ramki, G. Thiruppathi, Selva Kumar Ramasamy, P. Sundararaj, P. Sakthivel
Summary: A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semiaqueous solution. The probe exhibited enhanced fluorescence in its aggregated state and even higher fluorescence when chelated with Hg(II). The probe demonstrated high sensitivity and specificity for Hg(II) detection and was successfully applied for imaging Hg(II) in a living model.
Article
Biochemical Research Methods
Qun Zhang, Rui Yang, Gang Liu, Shiyan Jiang, Jiarui Wang, Juqiang Lin, Tingyin Wang, Jing Wang, Zufang Huang
Summary: This research aims to develop a cost-effective and portable method for measuring creatinine levels using the enhanced Tyndall effect phenomenon. The method offers a promising solution for monitoring renal healthcare in resource-limited settings.
