Journal
METHODS
Volume 46, Issue 3, Pages 194-203Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2008.09.013
Keywords
Acridine orange; Acidic Ca(2+) stores; Ca(2+); Confocal laser scanning microscope; Cyclic adenosine diphosphoribose (cADPR); Fluorescence; Lysosomes; Nicotinic acid adenine dinucleotide; phosphate (NAADP); pH; Sea urchin egg
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The body of literature characterizing cyclic adenosine diphosphoribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) as Ca(2+)-mobilizing second messengers is growing apace. However, their unique properties may, for the uninitiated, make them difficult to work with. This article reviews many of the available techniques (and associated pitfalls) for investigating these nucleotide messengers, predominantly focusing upon optical techniques using fluorescent reporters to measure Ca(2+) in the cytosol as well as Ca(2+) or pH within the lumen of intracellular organelles. (C) 2008 Elsevier Inc. All rights reserved.
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