Article
Chemistry, Multidisciplinary
Tao Peng, Jin Tian, Yuyan Zhao, Xu Jiang, Xiaoling Cheng, Guozhong Deng, Quan Zhang, Zhongqiang Wang, Jiawei Yang, Yongzheng Chen
Summary: This study achieved the cyclic deracemization of sulfoxides by combining the high-enantioselective enzyme MsrA with the low-enantioselective enzyme SMO, resulting in enantiopure sulfoxides. The cofactors of the enzymes were regenerated by the cascade catalysis of auxiliary enzymes, enabling high yield and enantioselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemistry & Molecular Biology
Lionel Tarrago, Alaattin Kaya, Hwa-Young Kim, Bruno Manta, Byung-Cheon Lee, Vadim N. Gladyshev
Summary: Methionine sulfoxide reductases (MSR) are enzymes that reduce methionine sulfoxide (MetO) back to methionine. MSRB1, a selenoprotein found in mammals, uses selenocysteine as a catalytic residue. It plays a crucial role in oxidative stress protection, neuronal cell maintenance, cognition, cancer cell proliferation, and immune response.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Maxence S. Vincent, Benjamin Ezraty
Summary: Methionine, a sulfur-containing residue in proteins, is prone to oxidation which can damage or activate protein function. Many bacteria possess enzymatic systems to reduce oxidized methionine, highlighting its reversible post-translational modification role. This review examines recent examples of methionine oxidation leading to protein loss or gain of function, and speculates on its role as a multilayer endogenous antioxidant system and its potential consequences for bacterial virulence.
MOLECULAR MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hayat Hage, Marie-Noelle Rosso, Lionel Tarrago
Summary: Research shows that most fungi possess genes for three types of methionine sulfoxide reductase: MsrA, MsrB, and fRMsr in their genomes. However, some fungi living in anaerobic environments or as obligate intracellular parasites lack these genes. Additionally, several horizontal gene transfer events from bacteria to fungi involving msr genes have been identified.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jana Schepers, Zorana Carter, Paraskevi Kritsiligkou, Chris M. Grant
Summary: This study reveals that methionine oxidation is a key factor in the formation of the yeast [PSI+] prion. By lacking methionine sulfoxide reductases, it is found that [PSI+] formation is increased, while lacking both isoenzymes of methionine sulfoxide reductases reduces [PSI+] formation.
Article
Biochemistry & Molecular Biology
Catherine Duport, Jean-Paul Madeira, Mahsa Farjad, Beatrice Alpha-Bazin, Jean Armengaud
Summary: The study reveals that under fermentative anoxic conditions, MsrA plays a significant role in antioxidant activities, affecting cell growth, motility, and energy metabolism.
Article
Biochemistry & Molecular Biology
Lihue N. Gonzalez, Matias S. Cabeza, Carlos Robello, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. Arias
Summary: Trypanosoma cruzi is a unicellular parasite that causes Chagas Disease and infects various mammalian hosts. It exhibits auxotrophy for L-Met and requires the amino acid from the extracellular environment of the host. The study identified a coding sequence for a free-R-MSR enzyme in T. cruzi, which is involved in the reduction of L-MetSO using specific cysteine residues. The results provide new insights into the redox metabolism of T. cruzi and contribute to understanding L-Met metabolism in this parasite.
Article
Food Science & Technology
Kristina Fleischer, Michael Hellwig
Summary: During the production of malt, cereal proteins undergo glycation and oxidation reactions. This study characterized malt proteins and found that the extent of oxidation increased with malt color. The results suggest that malt proteins are subject to oxidation during kilning, and further research is needed to determine the impact on the activity of malt.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Chemistry, Physical
Silvia Anselmi, Alexandra T. P. Carvalho, Angela Serrano-Sanchez, Jose L. Ortega-Roldan, Jill Caswell, Iman Omar, Gustavo Perez-Ortiz, Sarah M. Barry, Thomas S. Moody, Daniele Castagnolo
Summary: This study identifies selective and robust MsrA biocatalysts capable of efficiently catalyzing enantioselective reduction of various aromatic and aliphatic chiral sulfoxides at concentrations of 8-64 mM with high yields and excellent enantiomeric excess (up to 99%). Through rational mutagenesis design using in silico docking, molecular dynamics, and structural nuclear magnetic resonance (NMR) studies, a mutant enzyme MsrA33 was developed, which can catalyze the kinetic resolution of bulky sulfoxide substrates with non-methyl substituents on the sulfur atom with enantiomeric excess up to 99%, overcoming a significant limitation of current MsrA biocatalysts.
Article
Chemistry, Multidisciplinary
Yeting Guo, Jianxi Ying, Dongru Sun, Yumeng Zhang, Minyang Zheng, Ruiwen Ding, Yan Liu, Yufen Zhao
Summary: The yields of DKPs were found to be influenced by the sequence of linear dipeptides and the presence of trimetaphosphate in the reaction, suggesting that DKPs may play a key role in the origin of life.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Nikita Kuldyushev, Roland Schonherr, Ina Coburger, Marwa Ahmed, Rama A. Hussein, Eric Wiesel, Amod Godbole, Thorsten Pfirrmann, Toshinori Hoshi, Stefan H. Heinemann
Summary: This study introduces a genetically encoded probe for methionine oxidation using super-folder green fluorescent protein as a sensor, which enables the detection of methionine oxidation in living cells and organisms.
Article
Chemistry, Multidisciplinary
Sarah Bierbaumer, Luca Schmermund, Alexander List, Christoph K. Winkler, Silvia M. Glueck, Wolfgang Kroutil
Summary: In this study, a concurrent biocatalytic reduction and photocatalytic oxidation process was demonstrated. By coupling the enantioselective biocatalytic sulfoxide reduction and light-dependent sulfoxidation, a cyclic deracemization process for nonracemic sulfoxides was achieved with excellent conversions and optical purity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Applied
Amelie Boersig, Nevzat Konar, Sevim Dalabasmaz
Summary: Polyphenol-protein reactions in beta-lactoglobulin model solutions incubated with (-)-epicatechin were analyzed using microLC-timsTOF Pro-MS/MS combined with bioinformatics strategies. The addition of (-)-epicatechin resulted in changes in tryptic peptide profiles and covalent bond formation between (-)-epicatechin o-quinones and beta-LG. The study also identified significant oxidation promoted by the incubation of beta-LG with (-)-epicatechin, and the presence of (-)-epicatechin o-quinone in milk chocolate samples. This study has important implications for comprehensive food profiling strategies.
Article
Immunology
William N. Beavers, Ashley L. DuMont, Andrew J. Monteith, K. Nichole Maloney, Keri A. Tallman, Andy Weiss, Alec H. Christian, F. Dean Toste, Christopher J. Chang, Ned A. Porter, Victor J. Torres, Eric P. Skaar
Summary: Oxidative stress is a host strategy to control Staphylococcus aureus infections, with sulfur-containing amino acids being particularly susceptible to oxidation. Several systems have evolved in S. aureus to protect against protein oxidation or repair oxidized proteins.
INFECTION AND IMMUNITY
(2021)
Review
Chemistry, Multidisciplinary
Tao Peng, Xiaoling Cheng, Yongzheng Chen, Jiawei Yang
Summary: Chiral sulfoxides, valuable organosulfur compounds widely used in medicinal and organic synthesis, can be prepared through biocatalytic approaches, with sulfoxide reductases emerging as a new method with extraordinary catalytic properties. This review article discusses the chemical and biological functions of sulfoxide reductases and highlights their applications in chiral sulfoxide preparation.
FRONTIERS IN CHEMISTRY
(2021)
Article
Nutrition & Dietetics
Mark Krogh Hvistendahl, Rahim Mohammad Naimi, Svend Hoime Hansen, Jens Frederik Rehfeld, Hannelouise Kissow, Jens Pedersen, Lars Ove Dragsted, David Peick Sonne, Filip Krag Knop, Palle Bekker Jeppesen
Summary: The study evaluated the effects of glepaglutide on bile acid synthesis in patients with short bowel syndrome (SBS). Glepaglutide was found to stimulate the bile acid/FXR/FGF19 axis, leading to increased plasma concentrations of FGF19, which may play a role in preventing and treating intestinal failure-associated liver disease.
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
(2022)
Review
Agronomy
Signe M. Jensen, Felix M. Kluxen, Christian Ritz
Summary: The benchmark dose (BMD) methodology is increasingly recommended for risk assessment due to its advantages such as utilizing more information and being less dependent on tested dose levels, but it also requires expert knowledge and sophisticated statistical methods.
PEST MANAGEMENT SCIENCE
(2022)
Article
Gastroenterology & Hepatology
Nicola Prochazkova, Gwen Falony, Lars Ove Dragsted, Tine Rask Licht, Jeroen Raes, Henrik M. Roager
Summary: Accumulating evidence indicates that gut transit time is a key factor in shaping the gut microbiota composition and activity, which are linked to human health. This review discusses the variations in whole gut and segmental transit times, the impact of these variations on the gut microbiota, and the mechanisms by which the gut microbiota may affect gut motility. It argues that considering individual differences in gut transit time can improve our understanding of diet-microbiota interactions and disease-related microbiome signatures.
Review
Cardiac & Cardiovascular Systems
Trine L. Wilkens, Helle Sorensen, Majken K. Jensen, Jeremy D. Furtado, Lars O. Dragsted, Kenneth J. Mukamal
Summary: This study found a positive association between alcohol intake and HDL subspecies containing or lacking apoC3, apoE, and apoJ. Additionally, total levels of apoA1 and apoC3 were directly associated with alcohol consumption.
CURRENT PROBLEMS IN CARDIOLOGY
(2023)
Editorial Material
Nutrition & Dietetics
Elizabeth Mannion, Christian Ritz, Paola G. Ferrario
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2023)
Article
Nutrition & Dietetics
Joseph Mbabazi, Hannah Pesu, Rolland Mutumba, Gareth McCray, Kim F. Michaelsen, Christian Ritz, Suzanne Filteau, Andre Briend, Ezekiel Mupere, Benedikte Grenov, Henrik Friis, Mette Frahm Olsen
Summary: This study investigated the effects of milk protein (MP) versus soy and whey permeate (WP) versus maltodextrin in large-quantity, lipid-based nutrient supplement (LNS), as well as LNS itself versus no supplementation, on child development and head circumference among stunted children aged 1-5 years. The results showed that neither MP nor WP had an effect on any developmental domain. Although LNS itself had no impact on development, it resulted in a 0.07cm increase in head circumference.
Article
Nutrition & Dietetics
Suvi T. Kangas, Issa Niamanto Coulibaly, Zachary Tausanovitch, Bareye Ouologuem, Bethany Marron, Elizabeth Radin, Christian Ritz, Salimou Dembele, Cesaire T. Ouedraogo, Jeanette Bailey
Summary: The study aimed to determine the incidence of relapse and associated factors among children recovering from acute malnutrition using the ComPAS protocol. A cohort of 420 children with a MUAC >= 125 mm was monitored for 6 months. The cumulative incidence of relapse was 26.1% for MUAC < 125 mm and/or edema and 1.7% for MUAC < 115 mm and/or edema. Factors such as lower anthropometry at admission and discharge, more illness episodes per month, and certain socio-economic factors were associated with relapse.
Article
Nutrition & Dietetics
Sofie Hauerberg Henninger, Anna Yde Fibieger, Faidon Magkos, Christian Ritz
Summary: Current treatment options for overweight and obesity rely on strict diet and exercise regimes, making it difficult to sustain as a lifelong routine. This study explored the effects of mindful eating and YogaDance, individually and in combination, as alternative approaches to manage obesity. The findings suggest modest benefits of an 8-week combination of mindful eating and YogaDance, supporting previous research on the effectiveness of mindful eating, yoga, and dance.
Article
Nutrition & Dietetics
Alessia Trimigno, Bekzod Khakimov, Morten Arendt Rasmussen, Lars Ove Dragsted, Thomas Meinert Larsen, Arne Astrup, Soren Balling Engelsen
Summary: The New Nordic Diet (NND) has been found to have a positive impact on weight loss and blood pressure reduction in obese individuals. This study examined the differences in blood plasma metabolites and lipoprotein biomarkers between subjects who followed the Average Danish Diet (ADD) or NND. The findings suggest that the NND affects the plasma metabolome and lipoprotein profiles, with specific biomarkers such as HDL-1 cholesterol, apolipoprotein A1, phospholipids, and ketone bodies playing a significant role.
FRONTIERS IN NUTRITION
(2023)
Article
Nutrition & Dietetics
Nicola Prochazkova, Naomi Venlet, Mathias L. Hansen, Christian B. Lieberoth, Lars Ove Dragsted, Martin Bahl, Tine Rask Licht, Michiel Kleerebezem, Lotte Lauritzen, Henrik M. Roager
Summary: This study investigated the effects of whole-grain and refined-grain diets on gut fermentation and bowel function. The results showed that compared to the refined-grain diet, the whole-grain diet increased the levels of butyrate and caproate in feces, as well as stool frequency. The study also found that changes in gut microbiome were associated with fecal pH and colonic transit time.
FRONTIERS IN NUTRITION
(2023)
Article
Nutrition & Dietetics
Rolland Mutumba, Hannah Pesu, Joseph Mbabazi, Eva Greibe, Mette F. Olsen, Andre Briend, Christian Molgaard, Christian Ritz, Nicolette Nabukeera-Barungi, Ezekiel Mupere, Suzanne Filteau, Henrik Friis, Benedikte Grenov
Summary: This study assessed the correlates of iron, cobalamin, folate, and vitamin A biomarkers in stunted children in eastern Uganda. The results showed that these children have deficiencies in iron, cobalamin, and vitamin A. Therefore, interventions addressing stunting should also target these micronutrient deficiencies.
Article
Nutrition & Dietetics
Victor Nikiema, Suvi T. Kangas, Cecile Salpeteur, Andre Briend, Leisel Talley, Henrik Friis, Christian Ritz, Ebba Nexo, Adrian Mccann
Summary: In children with severe acute malnutrition, treatment with RUTF improved B12 status but did not fully correct deficiency. Breastfed children had lower B12 status compared to non-breastfed children.
Article
Nutrition & Dietetics
Rolland Mutumba, Joseph Mbabazi, Hannah Pesu, Eva Greibe, Mette F. Olsen, Andre Briend, Christian Molgaard, Christian Ritz, Ezekiel Mupere, Suzanne Filteau, Henrik Friis, Benedikte Grenov
Summary: Anemia is common among stunted children in eastern Uganda, and micronutrient deficiencies, inflammation, and malaria are associated with low hemoglobin levels.
Article
Nutrition & Dietetics
Kristina Pigsborg, Valdemar Stentoft-Larsen, Samuel Demharter, Mona Adnan Aldubayan, Alessia Trimigno, Bekzod Khakimov, Soren Balling Engelsen, Arne Astrup, Mads Fiil Hjorth, Lars Ove Dragsted, Faidon Magkos
Summary: A predictive model was developed using machine learning to predict successful weight loss on a New Nordic Diet. The study found that individual responses to weight loss vary and the optimal diet for successful weight loss may differ for different individuals. This research is important for improving precision dietary treatment for obesity.
FRONTIERS IN NUTRITION
(2023)
Article
Endocrinology & Metabolism
Diyora Kurmaeva, Yongxin Ye, Inal Bakhytkyzy, Violetta Aru, Dilbar Dalimova, Shahlo Turdikulova, Lars Ove Dragsted, Soren Balling Engelsen, Bekzod Khakimov
Summary: Uzbekistan has a high number of diet-related chronic diseases, possibly due to the high consumption of animal fats. Despite this, sheep meat, which is high in fats and acids, is considered healthy by locals. This study aimed to investigate the association between sheep meat intake frequency and blood plasma metabolites and lipoproteins in healthy Uzbek adults.
