Journal
METABOLOMICS
Volume 9, Issue 2, Pages 300-310Publisher
SPRINGER
DOI: 10.1007/s11306-012-0445-1
Keywords
Nitzschia closterium f. minutissima; Lipidomics; Growth phase; UPLC-Q-TOF-MS
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Funding
- National Natural Science Foundation of China [31172448]
- National Sparking Plan Project of China [2011GA701002]
- Zhejiang Public Welfare Project, China [2010C32021]
- Zhejiang Natural Science Foundation, China [Y3100534, Z3100565]
- Ningbo Science and Technology Research Projects, China [2011C11003, 2010C10054]
- Ningbo Marine Algae Biotechnology Team, China [2011B81007]
- K. C. Wong Magna Fund in Ningbo University
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Ultra Performance Liquid Chromatography-Electrospray ionization-Quadrupole-Time of Flight Mass Spectrometry (UPLC-ESI-Q-TOF-MS) is a powerful lipidomic tool. In this study, we developed a UPLC/Q-TOF-MS based method to investigate the lipid metabolomic changes in different growth phases of Nitzschia closterium f. minutissima. The data classification and biomarker selection were carried out by using multivariate statistical analysis, including principal components analysis (PCA), projection to latent structures with discriminant analysis (PLS-DA), and orthogonal projection to latent structures with discriminant analysis (OPLS-DA). We discovered that the intercellular lipid metabolites were significantly different among exponential, early stationary and late stationary phases. Thirty-one lipid molecules were selected and identified as putative biomarkers, including free fatty acid, Harderoporphyrin, phosphatidylglycerol, 1,2-diacyglycerl-3-O-4'-(N,N-trimethy)-homoserine, triacylglycerol, cholesterol, sulfoquinovosyldiacylglycerol, lyso-sulfoquinovosyldiacylglycerol, monogalactosyldiacylglycerol, digalactosyldiacylglycerol and lyso-digalactosyldiacylglycerol. These lipids have been shown previously to function in energy storage, membrane stability and photosynthesis efficiency during the growth of diatoms. Further analysis on the putative biomarkers demonstrated that nitrate starvation played critical role in the transition from exponential phase to stationary phase in N. closterium. This study is the first one to explore the lipidomic changes of microalgae in different growth phases, which promotes better understanding of their physiology and ecology.
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