Article
Multidisciplinary Sciences
Natalia O. Dranenko, Maria N. Tutukina, Mikhail S. Gelfand, Fyodor A. Kondrashov, Olga O. Bochkareva
Summary: The pathogenicity of Shigella and enteroinvasive Escherichia coli is based on the type 3 secretion system (T3SS) encoded by the pINV virulence plasmid. Recent genomic studies have found ipaH genes in potential marmot pathogens and E. coli from fecal samples of bovine calves, suggesting that non-human hosts may also be infected by these strains. Comparative genomic analysis revealed gene losses, duplications, and inconsistencies between strains phylogeny and composition of effectors, indicating horizontal gene transfer between E. coli adapted to different hosts.
SCIENTIFIC REPORTS
(2022)
Review
Microbiology
Ahtesham Ahmad Shad, Wajahat Ahmed Shad
Summary: S. sonnei is an emerging pathogen globally, with increasing prevalence and significant role in occupying ecological niches of other gut pathogens. Recent studies suggest that improved sanitation has reduced cross-immunization with other strains, while enhancing its competitive advantage through specific secretion systems.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Cell Biology
Lalitha Tadala, Dorothee Langenbach, Mirjam Dannborg, Ramon Cervantes-Rivera, Atin Sharma, Kevin Vieth, Lisa M. Rieckmann, Alkwin Wanders, David A. Cisneros, Andrea Puhar
Summary: This study demonstrates that the mechanosensitive plasma membrane channel PIEZO1 acts as a sensor for bacterial entry and initiates immune signaling through ATP secretion and gene expression. It suggests that the detection of infection by PIEZO1 is driven by physical signals instead of chemical ligands, independent of detection of microbial molecules.
Article
Engineering, Environmental
Wenshi Luo, Wencan Dai, Xiaochun Zhang, Li Zheng, Jianbin Zhao, Xiao Xie, Yanbin Xu
Summary: In this study, the toxicity of Shigella flexneri on Xenopus tropical embryos was evaluated. The results showed that exposure to S. flexneri increased embryo mortality, heart rate, and malformation rate, while decreasing hatching rate. It also led to misexpression of embryonic genes, oxidative stress, and immune responses. The study provides a theoretical reference for assessing the influence of Shigella on early amphibian embryos.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Ziquan Wang, Qian Xu, Siyuan Liu, Yingying Liu, Ying Gao, Meng Wang, Ling Zhang, Haiyan Chang, Qiang Wei, Zhiwei Sui
Summary: A rapid and quantitative method for multiplexed detection of Salmonella, E. coli O157:H7, and S. flexneri in ground beef was developed using a sample pretreatment protocol and flow cytometry, enabling analysis within 1 hour without the need for cultural enrichment. The method demonstrated good recovery rates for the three pathogens and had a detection range of 103 to 108 cells/g, with detection limits of 3.1 x 103 cells/g for Salmonella, 2.1 x 103 cells/g for E. coli O157:H7, and 2.3 x 103 cells/g for S. flexneri in ground beef.
Article
Microbiology
Junaid Iqbal, Niharika Malviya, Jennifer A. Gaddy, Chengxian Zhang, Andrew J. Seier, Kathryn P. Haley, Ryan S. Doster, Ana E. Farfan-Garcia, Oscar G. Gomez-Duarte
Summary: This study characterized the biofilm formation phenotype of BF-EIEC O96:H19 strain 52.1 isolated from children with moderate-to-severe gastroenteritis in Colombia, and identified the importance of pgaB and pgaC genes in N-acetylglucosamine-mediated biofilm formation. BF-EIEC O96:H19 exhibits both cell invasion and strong biofilm formation capabilities, which are associated with in vitro cell cytotoxicity.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Wieslaw Swietnicki
Summary: The review discusses strategies for designing vaccines against selected pathogens using bacterial secretory systems and connects them to the biology of the system. Examples are given for Y. pestis, S. enterica, B. anthracis, S. flexneri, and other human pathogens, and their effectiveness and long-term protection are discussed.
Article
Food Science & Technology
Ziquan Wang, Meng Wang, Qian Xu, Siyuan Liu, Ying Gao, Haiyan Chang, Zhiwei Sui
Summary: This study presents a novel highly sensitive flow cytometry-based method for detecting Salmonella, Escherichia coli O157, and Shigella flexneri in beef samples within 8 hours.
FOODBORNE PATHOGENS AND DISEASE
(2022)
Article
Immunology
Margaux Vienne, Marion Etiennot, Bertrand Escaliere, Justine Galluso, Lionel Spinelli, Sophie Guia, Aurore Fenis, Eric Vivier, Yann M. Kerdiles
Summary: NK cells are known to have cytotoxic effects on tumor cell lines, but their specific roles in primary tumor detection and elimination remain unclear. ILC1 play an active role in inhibiting the antitumoral immune response, suggesting the need to evaluate the tumor infiltration of ILC1 and NK cells to optimize immune harnessing in cancer therapies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta Cobo-Simon, Rowan Hart, Howard Ochman
Summary: Escherichia coli has been a vital model organism for studying various aspects of genetics, evolution, molecular biology, and pathogenesis. However, the classification of strains belonging to this species is inconsistent and unstable due to changes in the characteristics and criteria used to differentiate bacterial species. Furthermore, many isolates designated as E. coli are genetically more closely related to Shigella strains, resulting in the inclusion of the entire Shigella genus within the single species E. coli. By applying the biological species concept (BSC) and evaluating complete genomes, we found minimal barriers to gene flow among E. coli and Shigella strains, indicating the need for a more precise classification system.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Review
Immunology
Qifeng Ou, Rachael Power, Matthew D. Griffin
Summary: Regulatory T cells (Treg) play a critical role in maintaining immune homeostasis and have direct modulatory effects on innate immune responses in various acute and chronic diseases. Therapeutic interventions aimed at enhancing Treg numbers or potency show promising outcomes in autoimmunity and allogeneic transplants. Furthermore, interactions between Treg and innate immune effectors significantly impact disease severity and treatment. Treg-based therapeutic strategies hold potential for globally impactful inflammatory conditions such as type 2 diabetes, ischemia reperfusion injury, and atherosclerosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Xiaomei Zhang, Michael Payne, Thanh Nguyen, Sandeep Kaur, Ruiting Lan
Summary: Shigella and enteroinvasive Escherichia coli (EIEC) share similar invasion mechanisms and characteristics, making it difficult to differentiate between the two. Through genome analysis, this study identified multiple clusters of each pathogen and developed a new serotyping tool, ShigEiFinder, which showed high accuracy and specificity in distinguishing between Shigella and EIEC.
MICROBIAL GENOMICS
(2021)
Article
Immunology
Yu-Chen Wang, Yang Cao, Calvin Pan, Zhiqiang Zhou, Lili Yang, Aldons J. Lusis
Summary: This study utilized single-cell RNA sequencing and statistical modeling to investigate the dynamic properties and underlying cellular mechanisms of intestinal cells. It identified new cell subsets and developmental trajectories in different layers of intestinal cells. The high-fat high-sucrose Western diet led to changes in immune cell populations and nutrient absorption function in enterocytes, as well as revealed novel interactions and communication hubs among intestinal cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Review
Cell Biology
Xiaodong Du, Mingqian Li, Chen Huan, Guoyue Lv
Summary: This review discusses the origin, maturation, migration, and pathological effects of dendritic cell subsets in liver transplantation, as well as their roles in transplant rejection and tolerance. The latest progress in dendritic cell-based treatment regimens is also outlined.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Abdullah A. Alarfaj, Abdurahman Hajinur Hirad, Balasubramani Ravindran, Shilpa Perumal Narasimhamoorthi
Summary: This study successfully synthesized stable copper oxide nanoparticles using Aegle marmelos leaf extract and investigated their structural and antibacterial potential. The synthesized nanoparticles showed excellent antibacterial activity against Escherichia coli and Shigella flexneri, and complete plasmid DNA fragmentation was observed at higher concentration. Furthermore, in silico analysis confirmed the binding and interaction of active molecules in Aegle marmelos leaf extract with the BAM A transmembrane protein, suggesting their potential as drug candidates.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Microbiology
Leandro C. D. Breda, Isabela G. Menezes, Larissa N. M. Paulo, Sandro Rogerio de Almeida
Summary: Chromoblastomycosis (CBM) is a chronic and progressive subcutaneous mycosis caused by different species of fungi from the Herpotrichiellaceae family, often associated with agricultural activities. The economic challenges in treatment, lack of specific therapy, and potential for immunotherapeutic approaches make CBM a complex disease requiring further research and innovative treatment strategies.
Editorial Material
Microbiology
Leila M. Lopes-Bezerra, Sandro Rogerio de Almeida
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Microbiology
Paula Keiko Sato, Telma Miyuki Oshiro, Erika Cano Passos, Tatiana Giselle Rodrigues Miranda, Constancia Lima Diogo, Claudia de Abreu Fonseca, Aya Sadahiro, Sandro Rogerio de Almeida, Maria Aparecida Shikanai-Yasuda
Summary: The study investigated the effects of two Paracoccidioides brasiliensis antigens on monocyte-derived dendritic cells from PCM patients. Results showed that CFA-stimulated moDCs induced higher lymphoproliferation and cytokine release, suggesting that a complex antigen like CFA may be more effective in inducing a protective Th1 immune response.
Article
Mycology
Renato Massis Souza Campos, Grasielle Pereira Jannuzzi, Marcelo Augusto Kazuo Ikeda, Sandro Rogerio de Almeida, Karen Spadari Ferreira
Summary: Sporotrichosis is a subcutaneous mycosis distributed worldwide, mainly caused by traumatic inoculation of propagules. Research has shown that extracellular vesicles from Sporothrix brasiliensis play a protective role during infections by increasing fungicidal activity and elevating certain interleukin levels in mice. This indicates a potential therapeutic approach in utilizing EVs for protective effects during Sporothrix infections.
Article
Biochemistry & Molecular Biology
Bruna Felicio Milazzotto Maldonado Porchia, Luana Raposo de Melo Moraes Aps, Ana Carolina Ramos Moreno, Jamile Ramos da Silva, Mariangela de Oliveira Silva, Natiely Silva Sales, Rubens Prince dos Santos Alves, Clarissa Ribeiro Reily Rocha, Matheus Molina Silva, Karine Bitencourt Rodrigues, Tacita Borges Barros, Roberta Liberato Pagni, Patricia da Cruz Souza, Mariana de Oliveira Diniz, Luis Carlos de Souza Ferreira
Summary: The study demonstrates the effectiveness of combining active immunotherapy with cisplatin in treating advanced HPV-induced tumors. The combination treatment leads to complete tumor regression in mice and activates specific CD8+ T cells. The treatment also prevents tumor relapses and reduces the toxic effects of cisplatin.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Leandro Carvalho Dantas Breda, Cristiane Naffah de Souza Breda, Gilberto Hideo Kaihami, Jose Roberto Fogaca de Almeida, Grasielle Pereira Jannuzzi, Lucas Goncalves Ferreira, Sandro Rogerio de Almeida
Summary: Neutrophils play a complex role in F. pedrosoi infection, impacting the spread of the fungus and chronicity of the infection.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Brenda Kischkel, Camila Boniche-Alfaro, Isabela de Godoy Menezes, Suelen Andreia Rossi, Claudia Blanes Angeli, Sandro Rogerio de Almeida, Giuseppe Palmisano, Leila Lopes-Bezerra, Joshua D. Nosanchuk, Carlos Pelleschi Taborda
Summary: With over 6 million human deaths each year due to mycoses, there is an urgent need for fungal vaccines. This study used immunoproteomic and immunopeptidomic methods to map Histoplasma capsulatum epitopes, offering potential for cross-protective vaccines among different fungal species.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Roberta Liberato Pagni, Patricia da Cruz Souza, Rafael Pegoraro, Bruna Felicio Milazzotto Maldonado Porchia, Jamile Ramos da Silva, Luana Raposo de Melo Moraes Aps, Mariangela de Oliveira Silva, Karine Bitencourt Rodrigues, Natiely Silva Sales, Luis Carlos de Souza Ferreira, Ana Carolina Ramos Moreno
Summary: The study demonstrated that IL-6 and IDO negatively contribute to the activation of immune cells, particularly dendritic cells, reducing protective immune responses induced by the gDE7 vaccine. Therefore, combined strategies based on inhibition of IL-6 and IDO offer new perspectives for immunotherapy against HPV-related tumors.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Oncology
Roberta Liberato Pagni, Guilherme Formoso Pelegrin, Ana Carolina Ramos Moreno
TRANSLATIONAL CANCER RESEARCH
(2022)
Article
Cell Biology
Jamile Ramos da Silva, Karine Bitencourt Rodrigues, Guilherme Formoso Pelegrin, Natiely Silva Sales, Hiromi Muramatsu, Mariangela de Oliveira Silva, Bruna F. M. M. Porchia, Ana Carolina Ramos Moreno, Luana Raposo M. M. Aps, Alexia Adrianne Venceslau-Carvalho, Istvan Tombacz, Wesley Luzetti Fotoran, Katalin Kariko, Paulo J. C. Lin, Ying K. Tam, Mariana de Oliveira Diniz, Norbert Pardi, Luis Carlos de Souza Ferreira
Summary: This study compared the performance of three different mRNA vaccine modalities in targeting tumors associated with HPV-16 infection in mice. The results showed that all three vaccines effectively activated E7-specific CD8+ T cells, generated memory T cell responses, prevented tumor relapses, and eradicated subcutaneous tumors at different growth stages. In addition, the mRNA-LNP vaccines showed potent tumor protection in two different mouse tumor models. Compared to DNA and recombinant protein vaccines, the mRNA vaccines demonstrated superior immunogenicity and therapeutic efficacy. These findings support further evaluation of these mRNA vaccines in clinical trials.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Microbiology
Suelen S. Santos, Eline Rampazo, Carlos P. Taborda, Joshua D. Nosanchuk, Silvia B. Boscardin, Sandro R. Almeida
Summary: Paracoccidioidomycosis (PCM) is a systemic mycosis caused by Paracoccidioides brasiliensis, a common endemic fungus in Latin American countries. Vaccines targeting strong T cell-mediated immune responses are being developed. The P10 peptide derived from the fungus has shown promise in inducing immune responses when targeted to dendritic cells (DC) with a monoclonal antibody to the DEC205 receptor. In experiments with mice, treatment with the aDEC/P10 chimeric antibody resulted in increased IFN-γ and IL-4 levels, reduced fungal burdens, and normal lung tissue architecture. This strategy shows potential for vaccination and therapy against PCM.
Article
Multidisciplinary Sciences
Victor Bolsanelli Cioffi, Maria Fernanda de Castro-Amarante, Aleksei Lulla, Robert Andreata-Santos, Mario Costa Cruz, Ana Carolina Ramos Moreno, Mariangela de Oliveira Silva, Bianca de Miranda Peres, Lucio Holanda Gondim de Freitas Junior, Carolina Borsoi Moraes, Edison Luiz Durigon, Nicola Coker Gordon, Marko Hyvoenen, Luis Carlos de Souza Ferreira, Andrea Balan
Summary: This study utilized the RAD display system to present peptides derived from the SARS-CoV-2 Spike protein, leading to the detection of specific antibodies and the generation of inhibitory antibodies against viral infection. The RAD display system shows promise as a platform for peptide-based vaccine antigen design, epitope-specific antibody mapping, and the development of diagnostic and therapeutic antibodies.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Ana Carolina Ramos Moreno, Natalia Pasternak Taschner, Marco Aurelio Floriano Piantola, Barbara Rodrigues Cintra Armellini, Camilo Lellis-Santos, Rita de Cassia Cafe Ferreira
Summary: Traditional lab classes in microbiology are common in educational institutions. In order to provide a more authentic learning experience, the Real-Lab-Day was developed as a multimodal learning experience for undergraduate students to develop competencies, critical analysis, and teamwork skills. Students were mentored by graduate students in research laboratories and conducted scientific assays to address questions in microbiology. The Real-Lab-Day improved students' learning and interest in microbiology research, and was highly approved as a teaching tool.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Mariangela O. Silva, Bianca S. Almeida, Natiely S. Sales, Mariana O. Diniz, Luana R. M. M. Aps, Karine B. Rodrigues, Jamile R. Silva, Ana C. R. Moreno, Bruna F. M. M. Porchia, Fernando B. Sulczewski, Silvia B. Boscardin, Luis C. S. Ferreira
Summary: The delivery of HPV tumor antigens to DCs, particularly via the DEC205 surface receptor, is a promising therapeutic approach, providing new opportunities for the development of alternative immunotherapies for patients with HPV-associated tumors.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Oncology
Jamile Ramos da Silva, Ana Carolina Ramos Moreno, Natiely Silva Sales, Mariangela de Oliveira Silva, Luana R. M. M. Aps, Bruna F. M. M. Porchia, Karine Bitencourt Rodrigues, Natalia Cestari Moreno, Alexia Adrianne Venceslau-Carvalho, Carlos Frederico M. Menck, Mariana de Oliveira Diniz, Luis Carlos de Souza Ferreira
Summary: The combination of Gem and active immunotherapy demonstrates synergistic antitumor effects, providing evidence for a new and feasible chemoimmunotherapeutic strategy in cancer treatment.
