Journal
MEMORIAS DO INSTITUTO OSWALDO CRUZ
Volume 106, Issue 8, Pages 1014-1016Publisher
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762011000800019
Keywords
Trypanosoma cruzi; cell invasion; cytoskeleton; membrane protrusion
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Funding
- FAPESP
- CNPq
- CAPES
- NIH
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It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
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