Article
Infectious Diseases
Maria Evangelina Primo, Julio Bellezze, Nicolas Morel, Matilde Mazzucco Panizza, Beatriz Susana Valentini, Susana Marta Torioni, Carolina Soledad Thompson
Summary: A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the highly conserved msp5 gene of Anaplasma spp. was developed and evaluated in field samples. The assay showed high sensitivity and agreement with other specific nPCR methods for the detection of A. marginale and A. centrale.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Infectious Diseases
Munir Aktas, Sezayi Ozubek
Summary: The genetic diversity of Anaplasma marginale in dairy cows from Turkey was investigated using microsatellites and tandem repeats of the msp1? gene. Different genotypes were identified in cows under different management systems, with high infection potential observed in some genotypes. Phylogenetic analysis revealed clustering of A. marginale strains from Turkey with strains from different continents, suggesting a history of cattle trade and transport.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Veterinary Sciences
Hassan Y. A. H. Mahmoud, Alsagher O. Ali, Tetsuya Tanaka
Summary: In this study, novel molecular techniques were used to detect and characterize A. marginale in cattle, buffalo, and camel populations in southern Egypt. The prevalence of A. marginale infection was found to be 61% in cattle, 12% in buffaloes, and 6.66% in camels. Phylogenetic analysis provided new data on A. marginale infections in camels. Screening for A. marginale is recommended even in the absence of clinical signs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Parasitology
Joshua Kamani, Abdul Matin M. N. Mubo Qasim, Toyin A. Olubade, Moses Samuel Abasiama, Ali Gajibo, Peter Umar Balami, Mike Shands, Shimon Harrus
Summary: Bovine anaplasmosis caused by Anaplasma marginale is a widespread and significant endemic disease in developing countries. This study analyzed samples from Nigerian cattle and identified three different nucleotide sequence types of A. marginale, providing the first nationwide report on its diversity and genetic characterization.
Article
Immunology
Dhruba Das, Kalyan Sarma, Chethan Gollahalli Eregowda, Parimal Roychoudhury, Justus Babykutty Rajesh, Parthasarathi Behera, Hridayesh Prasad, Hniang Lalrinkima, Fatema Aktar, Nikitasha Bora, Champak Deka, Neeraj Thakur, Thingujam Chaa Tolenkhomba
Summary: This study aimed to investigate the distribution of Anaplasma species infection in cattle from the Aizawl region of Mizoram, India, and analyze the associated risk factors. The study also identified haemato-biochemical alterations, oxidant/antioxidant status, and serum trace mineral levels in cattle with naturally occurring Anaplasma marginale infection.
MICROBIAL PATHOGENESIS
(2022)
Article
Veterinary Sciences
Mariana da Silva Casa, Guillermo Giovambattista, Graziela Vieira Fonteque, Ellen Lara Miguel, Carla Ivane Ganz Vogel, Luiz Claudio Miletti, Shin-Nosuke Takeshima, Joandes Henrique Fonteque
Summary: This study investigated the association between BoLA-DRB3 gene and infections of Anaplasma marginale, Babesia bovis, and Babesia bigemina in Crioulo Lageano cattle using PCR-SBT and gene sequencing. The results showed that two alleles were significantly associated with resistance to A. marginale infection, one allele was associated with resistance to B. bovis infection, and none of the alleles were associated with resistance to B. bigemina infection.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Witchuta Junsiri, Amaya Watthanadirek, Napassorn Poolsawat, Sutthida Minsakorn, Nitipon Srionrod, Pornpiroon Nooroong, Siriphan Sangchuai, Runglawan Chawengkirttikul, Kittirat Glab-ampai, Panat Anuracpreeda
Summary: The study found that MSP2 of A. marginale exhibits high variability between different strains, with the identification of 10 different msp2 gene haplotypes. High entropy peaks were observed in the nucleic and amino acid sequences, with MHC-II alleles representing the hypervariable region of MSP2. The distribution of AmMSP2 on infected erythrocyte membranes and outside was demonstrated, indicating the potential use of rMSP2 for immunodiagnosis and vaccine development against anaplasmosis.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Alberto Moraga Fernandez, Jose Antonio Ortiz, Abdul Jabbar, Abdul Ghafar, Alejandro Cabezas-Cruz, Gabriela de la Fuente, Jose de la Fuente, Isabel G. Fernandez de Mera
Summary: Anaplasma marginale is a bacterium capable of causing disease in animals and humans, and its occurrence has been detected in various regions around the world. A study conducted in southwestern Spain identified clinical cases of bovine anaplasmosis and characterized the bacteria using molecular-phylogenetic methods, revealing the presence of novel genotypes and coexistence of different strains.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Infectious Diseases
Maria Evangelina Primo, Macarena Sarli, Carolina Soledad Thompson, Susana Marta Torioni, Ignacio Eduardo Echaide
Summary: This study evaluated the impact of long-acting oxytetracycline treatment on A. marginate genotypes, revealing different sensitivities to OTC and the importance of other variables for successfully sterilizing carriers.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Veterinary Sciences
Remil L. Galay, Carina R. Llaneta, Maria Karla Faye B. Monreal, Antero L. Armero, Arianne Bel D. Baluyut, Czarina Marie F. Regino, Kristina Andrea C. Sandalo, Billy P. Divina, Melbourne R. Talactac, Lennox P. Tapawan, Maarten Czar L. Mojares, Cherry R. Alvarez, Emmanuel R. Mago, Noemi D. Encarnacion, Masako Andoh, Tetsuya Tanaka
Summary: The study investigated the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines. It found a high prevalence of A. marginale in the region and provided the first molecular evidence of E. minasensis in the country.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Amaya Watthanadirek, Witchuta Junsiri, Sutthida Minsakorn, Napassorn Poolsawat, Nitipon Srionrod, Pacharaporn Khumpim, Runglawan Chawengkirttikul, Panat Anuracpreeda
Summary: Anaplasmosis is a tick-borne disease affecting cattle and other ruminants, resulting in significant economic losses worldwide. The study focused on the MSP5 protein of A. marginale, identifying multiple haplotypes and conserving epitopes that could be used for immunodiagnostic assays and vaccine development. Recombinant MSP5 showed promise for controlling anaplasmosis.
Article
Entomology
Matilde N. Mazzucco Panizza, Maria Rossner, Marcelo L. Signorini, Santiago Nava
Summary: The aim of this study was to quantitatively analyze the migration of Rhipicephalus microplus ticks among cattle in different stocking conditions, and discuss its role in the epidemiology of Anaplasma marginale. The results showed that migration of R. microplus occurs under field conditions and is associated with stocking rates. These findings highlight the significance of R. microplus in the epidemiology of A. marginale through its role as a vector for intrastadial transmission.
MEDICAL AND VETERINARY ENTOMOLOGY
(2023)
Article
Veterinary Sciences
Julio Bellezze, Carolina S. S. Thompson, Anabela S. S. Bosio, Susana M. M. Torioni, Maria E. Primo
Summary: Immunization of calves with Anaplasma centrale can prevent acute anaplasmosis caused by A. marginale. A displacement, double-antigen, sandwich ELISA (ddasELISA) was developed to detect antibodies against A. marginale or A. centrale. The ddasELISA was validated and shown to be effective for detecting A. marginale infection.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Veterinary Sciences
Maria Augusta Chavez-Larrea, Cristina Cholota-Iza, Jorge Cueva-Villavicencio, Michelle Yugcha-Diaz, Jorge Washington Ron-Roman, Andrea Rodriguez-Cabezas, Claude Saegerman, Armando Reyna-Bello
Summary: This study molecularly identified and genotyped Trypanosoma theileri isolated from cattle in Ecuador through the analysis of CatL and ITS sequences. It also found a high frequency of coinfection of this haemoparasite with other blood haemotropic organisms.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Farhan Ahmad Atif, Rao Zahid Abbas, Saba Mehnaz, Muhmmad Fiaz Qamar, Kashif Hussain, Muhammad Usman Nazir, Muhammad Arfan Zaman, Aman Ullah Khan, Mourad Ben Said
Summary: This study found that Theileria annulata and Anaplasma marginale infections are prevalent in bovine populations in Jhang and Rawalpindi districts of Punjab, Pakistan. The overall prevalence of these two tick-borne pathogens was 19.79%. Factors such as age, breed, tick infestation, history of tick-borne diseases, frequency of acaricidial application, and season were significantly associated with tick-borne infections.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2022)
Article
Microbiology
Natalia Lins Silva-Gomes, Rita de Cassia Pontello Rampazzo, Claudia Maria do Nascimento Moreira, Gabriane Nascimento Porcino, Cyndia Mara Bezerra dos Santos, Marco Aurelio Krieger, Eveline Gomes Vasconcelos, Stenio Perdigao Fragoso, Otacilio C. Moreira
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Veterinary Sciences
Henrique A. Bomjardim, Mariana C. Oliveira, Matheus D. Cordeiro, Marilene F. Brito, Adivaldo Henrique Fonseca, Carlos M. C. Oliveira, Natalia S. Silva, Jose D. Barbosa
PESQUISA VETERINARIA BRASILEIRA
(2020)
Article
Infectious Diseases
Michele Bahia, Lucas Teixeira da Silva, Bruno Montijo da Silva, Matheus dias Cordeiro, Alexandro Guterres, Claudia Bezerra da Silva, Jenevaldo Barbosa da Silva, Adivaldo Henrique Fonseca
Summary: The study identified 13 different strains of A. marginale, with the alpha-beta-F-F-F strain present in all animals with acute anaplasmosis and in three asymptomatic animals.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Cell Biology
Hellida Marina Costa-Silva, Bruno Carvalho Resende, Adriana Castilhos Souza Umaki, Willian Prado, Marcelo Santos da Silva, Stela Virgilio, Andrea Mara Macedo, Sergio Danilo Junho Pena, Erich Birelli Tahara, Luiz Ricardo Orsini Tosi, Maria Carolina Elias, Luciana Oliveira Andrade, Joao Luis Reis-Cunha, Gloria Regina Franco, Stenio Perdigao Fragoso, Carlos Renato Machado
Summary: DNA topoisomerases, in particular topoisomerase 3 alpha, play crucial roles in genomic maintenance, cell growth, and DNA damage response in Trypanosoma cruzi. Knockout of topoisomerase 3 alpha affects parasite growth, invasion rates, and ability to repair DNA damage induced by genotoxic agents. These findings underscore the importance of topoisomerase 3 alpha in homologous recombination repair and replication stress in T. cruzi.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Diego Rodney Rodrigues de Assis, Alexandre Almeida Oliveira, Samuel Luiz Porto, Rayane Aparecida Nonato Rabelo, Eduardo Burgarelli Lages, Viviane Correa Santos, Matheus Marques Milagre, Stenio Perdigao Fragoso, Mauro Martins Teixeira, Rafaela Salgado Ferreira, Carlos Renato Machado, Lucas Antonio Miranda Ferreira, Nivaldo Lucio Speziali, Heloisa Beraldo, Fabiana Simao Machado
Summary: (English Summary:)
This study investigated the potential antichagasic activities of thiosemicarbazones and hydrazones as new anti-T. cruzi drug candidates. Compounds C1 and C3 showed anti-parasitic activity in macrophages without toxicity to host cells, and were also effective in directly killing trypomastigotes. Moreover, C1 and C3 attenuated parasitemia in T. cruzi-infected mice and maintained anti-T. cruzi activity in vivo when loaded in a lipid nanocarrier system.
BIOORGANIC CHEMISTRY
(2021)
Article
Veterinary Sciences
Ana Paula Gori Palka, Tatiana Reichert Assuncao de Matos, Claudemir de Souza, Danilo Santos Eugenio, Marco Aurelio Krieger, Stenio Perdigao Fragoso, Daniela Parada Pavoni
Summary: The study demonstrated that recombinant VP3 (rVP3) showed high sensitivity and specificity as a diagnostic reagent in an enzyme-linked immunosorbent assay (ELISA) compared to a commercial test, especially for vaccinated animals. rVP3 was able to detect antibodies in non-vaccinated chickens, potentially developed against circulating IBDV strains. Evaluation of VP3 full-length protein can be used to assess post-vaccination immunological status of chickens with low cost.
BMC VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Felipe S. Coelho, Mauricio M. Oliveira, Danielle P. Vieira, Pedro H. M. Torres, Isabel C. F. Moreira, Erica S. Martins-Duarte, Ines C. Goncalves, Adriana Cabanelas, Pedro G. Pascutti, Stenio P. Fragoso, Angela H. Lopes
Summary: The study identified a receptor in T. cruzi that interacts with PAF and LPC, influencing cellular differentiation and infection mechanisms of the parasite.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Juliana Nunes Roson, Marcela de Oliveira Vitarelli, Hellida Marina Costa-Silva, Kamille Schmitt Pereira, David da Silva Pires, Leticia de Sousa Lopes, Barbara Cordeiro, Amelie Kraus, Karin Navarro Tozzi Cruz, Simone Guedes Calderano, Stenio Perdigao Fragoso, T. Nicolai Siegel, Maria Carolina Elias, Julia Pinheiro Chagas da Cunha
Summary: This study focused on the unique features of gene expression regulation in trypanosomatids. Through histone ChIP-seq analysis, it was found that H2B.V was enriched at critical genomic regions in T. cruzi and interacted with chromatin acetylation factors. Parasites with reduced H2B.V levels showed higher rates of differentiation and infectivity.
Article
Microbiology
Filipe Martins Santos, Nayara Yoshie Sano, Sany Caroline Liberal, Maria Augusta Dario, Wesley Arruda Gimenes Nantes, Fernanda Moreira Alves, Alanderson Rodrigues da Silva, Carina Elisei De Oliveira, Andre Luiz Rodrigues Roque, Heitor Miraglia Herrera, Ana Maria Jansen
Summary: This study evaluated the diversity of kinetoplastids infecting small mammals in the forest patches of the Pantanal biome. Through hemocultures and Next Generation Sequencing, eight parasite species were identified, including Leishmania amazonensis, L. infantum and Trypanosoma cascavelli. Additionally, the study revealed the presence of L. infantum and L. amazonensis infections in small mammals from this region for the first time.
Article
Agronomy
Jessica Cecilia Pinheiro Rodrigues, Isabela de Souza Parry, Thais de Cassia Mouzinho Lopes, Eder Barros dos Santos, Livia Caricio Martins, Adivaldo Henrique da Fonseca, Paulo Cesar Magalhaes-Matos
Summary: In the Iguacu National Park, some free-living coatis have antibodies against different types of arboviruses, indicating a potential transmission cycle in the area.
Article
Agriculture, Multidisciplinary
Matheus Dias Cordeiro, Izabela Mesquita Arajo, Claudia Bezerra da Silva, Alexandro Guterres, Adivaldo Henrique da Fonseca, Bruna de Azevedo Bata
Summary: The present study used PCR to detect tick-borne hemoparasites in the blood of wild Didelphis aurita, and found Theileria bicornis DNA. This challenges us to further study marsupial piroplasmosids and their potential involvement in zoonoses.
SEMINA-CIENCIAS AGRARIAS
(2022)
Article
Biochemistry & Molecular Biology
Edlainne Pinheiro Ferreira-Sena, Daiana de Jesus Hardoim, Flavia de Oliveira Cardoso, Luiz Ney d'Escoffier, Isabela Ferreira Soares, Joao Pedro Rangel da Silva Carvalho, Ricardo Almir Angnes, Stenio Perdigao Fragoso, Carlos Roberto Alves, Salvatore Giovanni De-Simone, Josue da Costa Lima-Junior, Alvaro Luiz Bertho, Tania Zaverucha-do-Valle, Franklin Souza da Silva, Katia da Silva Calabrese
Summary: Leishmaniasis is a complex and diverse disease that is considered a major public health problem. Despite the availability of treatment options, there are currently no vaccines for cutaneous leishmaniasis. This study focused on the prediction and characterization of antigenic epitopes that could interact with major histocompatibility complex class I in mice or humans. Through a combination of immunogenicity prediction and interaction assays, nine antigenic peptides were identified as strong candidates for the development of a peptide vaccine against leishmaniasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biodiversity Conservation
Felipe Pereira Bisaggio, Lennon de Souza Malta, Fernando Paiva, Carina Elisei, Luiz Eduardo Roland Tavares
Summary: This study evaluated the parasite community of Gymnogeophagus balzanii for the first time, finding ten taxa including nematodes, digenea, copepods, and monogenea. The community had low richness, diversity, and evenness, with few specialist species and several generalists dominating. Host size and weight, as well as sex, were strong determinants of the parasite community. The parasites did not affect host body condition, but the presence of Clinostomum metacercariae is important for human health issues.
JOURNAL OF NATURAL HISTORY
(2023)
Article
Agriculture, Multidisciplinary
Jose Luis Rodrigues Bautista, Matheus Dias Cordeiro, Izabela Mesquita Araujo, Paula de Abreu Moraes, Jaqueline Rodrigues de Almeida Valin, Juliana de Almeida Coelho, Tays Figueiroa, Gilliard Ferreira, Adivaldo Henrique da Fonseca
Summary: This study evaluated the tick species and tick-borne pathogens in forest fragments in a rural area of Divino, Minas Gerais state, Brazil. After morphological identification and DNA testing of ticks, no DNA from various pathogens was detected.
SEMINA-CIENCIAS AGRARIAS
(2022)
Article
Agriculture, Multidisciplinary
Marcio Barizon Cepeda, Matheus Dias Cordeiro, Bruna de Azevedo Baeta, Adivaldo Henrique da Fonseca
Summary: This study aimed to establish the infection dynamics of B. anserina in experimentally infected Gallus gallus and determine its impact on the health and weight gain of the animals. The number of spirochetes in the blood was directly proportional to the appearance of clinical signs and symptoms, indicating the severity of infection.
SEMINA-CIENCIAS AGRARIAS
(2021)