Article
Public, Environmental & Occupational Health
Arshi Islam, Farah Deeba, Bansidhar Tarai, Ekta Gupta, Irshad H. Naqvi, Mohd. Abdullah, Ravins Dohare, Anwar Ahmed, Fahad N. Almajhdi, Tajamul Hussain, Shama Parveen
Summary: Evolutionary analysis of Dengue virus reveals genotypic variations and serotype shifts, which can lead to future outbreaks. The study collected and analyzed 250 samples from 2017 and 2018, as well as 800 sequences from global sources. Predominant genotypes were identified for DENV-1, DENV-3, and DENV-4. The findings provide valuable insights for vaccine development against Dengue virus.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Virology
Aung Min Soe, Mya Myat Ngwe Tun, Takeshi Nabeshima, Theingi Win Myat, Moh Moh Htun, Htin Lin, Nang Sarm Hom, Shingo Inoue, Khine Mya Nwe, Lynn Pa Pa Aye, Mizuki Fukuta, Kyaw Zin Thant, Futoshi Hasebe, Kouichi Morita, Sujan Shresta, Hlaing Myat Thu, Meng Ling Moi
Summary: This study analyzed DENV-1, DENV-2, DENV-3, and DENV-4 serotypes in 1235 serum samples collected in Myanmar between 2017 and 2019, identifying the emergence of DENV-3 genotype-I and its persistence over the years. Despite the changes in serotype dynamics and increased DENV-3 cases, no significant differences were found in viral loads, disease severity, and infection status of individuals infected with different DENV serotypes during the 3-year study.
Article
Infectious Diseases
Praveen Rai, Sonam Kille, Akshatha Kotian, Ballamoole Krishna Kumar, Vijaya Kumar Deekshit, Mysore Shivalingappa Ramakrishna, Indrani Karunasagar, Iddya Karunasagar
Summary: The study examined the circulating dengue virus and serotypes in patients in Mysuru district, Southern India during disease outbreak. Results showed DENV-4 as the predominant serotype in 2016, while DENV-2, DENV-3, and DENV-1 emerged in 2017. The study highlights the importance of genetic diversity in monitoring and controlling dengue infection.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Infectious Diseases
Snigdha Rahman Titir, Shyamal Kumar Paul, Salma Ahmed, Nazia Haque, Syeda Anjuman Nasreen, Khondoker Shoaib Hossain, Fahim Uddin Ahmad, Sultana Shabnam Nila, Jobyda Khanam, Neaz Nowsher, Abu Mohammod Mayeenuddin Al Amin, Amdad Ullah Khan, Meiji Soe Aung, Nobumichi Kobayashi
Summary: In 2019, in Bangladesh, DENV-3 genotype I was predominant, with the identification of DENV-3 genotype III. These findings suggest an increased incidence of recent secondary infection of dengue fever in Bangladesh in 2019.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2021)
Article
Infectious Diseases
Haoyu Long, Chi Zhang, Cai Chen, Jing Tang, Bing Zhang, Yinghan Wang, Jiali Pang, Wenzhe Su, Kuibiao Li, Biao Di, Yao-Qing Chen, Yuelong Shu, Xiangjun Du
Summary: This study designed a sequence-based pipeline to study the transmission of dengue, identifying limited intercontinental transmission and quantifying regional circulation.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Muhammad Amir, Abrar Hussain, Muhammad Asif, Sagheer Ahmed, Hina Alam, Marius Alexandru Moga, Maria Elena Cocuz, Luigi Marceanu, Alexandru Blidaru
Summary: Dengue fever, caused by the Dengue virus (DENV), is a common vector-borne disease belonging to the Flaviviridae family. DENV infects various vertebrates and arthropods, causing diseases in birds, wild animals, and primates. A study on different geographical strains of DENV-3 revealed significant variations in genome size and amino acid sequences.
Article
Infectious Diseases
Abderrahman Bimouhen, Zakia Regragui, Fatima El Falaki, Hassan Ihazmade, Samira Benkerroum, Amal Barakat, Ahmed Rguig, Touria Benamar, Soumia Triki, Youssef Bakri, Hicham Oumzil
Summary: This study investigated the prevalence and characteristics of HRSV in Morocco, as well as the circulating genotypes between 2012 and 2017. The results showed that BA9, BA10, and ON1 genotypes were the most commonly detected genotypes during this period. Continuous genomic surveillance is needed to monitor the variations in the virus.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2023)
Letter
Immunology
Marta Giovanetti, Luiz Augusto Pereira, Gilberto A. Santiago, Vagner Fonseca, Maria Paquita Garcia Mendoza, Carla de Oliveira, Laise de Moraes, Joilson Xavier, Stephane Tosta, Hegger Fristch, Emerson de Castro Barbosa, Evandra Strazza Rodrigues, Dana Figueroa-Romero, Carlos Padilla-Rojas, Omar Caceres-Rey, Ana Flavia Mendonca, Fernanda de Bruycker Nogueira, Rivaldo Venancio da Cunha, Ana Maria Bispo de Filippis, Carla Freitas, Cassio Roberto Leonel Peterka, Carlos Frederico Campelo de Albuquerque, Leticia Franco, Jairo Andres Mendez Rico, Jorge L. Munoz-Jordan, Vinicius Lemes da Silva, Luiz Carlos Junior Alcantara
Summary: In this study, a cosmopolitan genotype of dengue virus serotype 2 was identified in a 56-year-old male patient from the state of Goias in Brazil using nanopore sequencing and phylogenetic analysis. The emergence of this genotype in Brazil highlights the need for risk assessment and surveillance to mitigate its epidemic potential.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Virology
Rabia Hakim, Shaheen Bibi, Qasim Ali, Bisma Sarfraz, Yasir Arshad, Zaira Rehman, Muhammad Salman, Aamer Ikram, Massab Umair
Summary: The aim of this study was to identify the prevalent dengue serotypes during the major outbreak in Pakistan in 2021. Out of 373 serum samples tested, 273 (73%) were positive for dengue virus infection, with a predominance of DENV-2 serotype (99%) and a single case of DENV-1. Sequencing data revealed the presence of a cosmopolitan genotype within the DENV-2 Pakistani isolates. This study highlights the persistent predominance of DENV-2 during the 2021 outbreak, emphasizing the need to improve surveillance strategies and vector breeding control programs in Pakistan.
Review
Virology
Tiantian Wu, Zhongdao Wu, Yi-Ping Li
Summary: Infection with dengue virus (DENV) can lead to a range of symptoms from dengue fever to severe dengue, causing a significant burden on global populations, especially in tropical and subtropical regions. The four serotypes of DENV have been identified in China, leading to frequent outbreaks in southern and coastal provinces. Despite the lack of antiviral drugs and approved vaccines, strategies such as public awareness and mosquito control have helped reduce the prevalence of dengue in China.
REVIEWS IN MEDICAL VIROLOGY
(2022)
Article
Microbiology
Hua Sun, Wenwu Yao, Abubakar Siddique, Fan He, Min Yue
Summary: This study aimed to investigate the genetic diversity and molecular evolution of the dengue virus in Hangzhou, China, during and after a dengue fever epidemic. The results showed that the virus strains from 2017 were imported from Singapore, Malaysia, and Thailand, while local strains from 2018 and 2019 were closely associated with Guangzhou isolates, indicating within-country transmission. Various amino acid substitutions and recombination events were observed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Sandesh Rimal, Sabin Shrestha, Kishor Pandey, Thanh Vu Nguyen, Parmananda Bhandari, Yogendra Shah, Dhiraj Acharya, Nabaraj Adhikari, Komal Raj Rijal, Prakash Ghimire, Yuki Takamatsu, Basu Dev Pandey, Stefan Fernandez, Kouichi Morita, Mya Myat Ngwe Tun, Shyam Prakash Dumre
Summary: The largest dengue outbreak in Nepal's history occurred in 2022, affecting all 77 districts. The capital, Kathmandu, was the hardest hit. The epidemic had a new serotype addition and multiple circulating serotypes, leading to a higher frequency of hospitalizations, more severe dengue, and more deaths than in the past.
Article
Microbiology
Juthamas Phadungsombat, Huong Thi Thu Vu, Quynh Thi Nguyen, Ha Thi Van Nguyen, Ha Thi Nhu Nguyen, Bich Thi Dang, Emi E. Nakayama, Azumi Ishizaki, Hiroshi Ichimura, Tatsuo Shioda, Thach Ngoc Pham
Summary: The study conducted molecular characterization of dengue patients' samples in northern Vietnam collected in 2019-2020, revealing the circulating serotypes as DENV-1 and DENV-2, with DENV-2 being the predominant strain. The current Asian-Pacific lineage of the cosmopolitan virus is closely related to recent outbreaks in Southeast Asian countries and China. Multiple introductions of the virus occurred in 2016-2017, possibly from maritime Southeast Asia, mainland Southeast Asia, or China, rather than an expansion of localized Vietnamese cosmopolitan strains.
Article
Virology
Jorge Alonso Rivera, Aura Caterine Rengifo, Alicia Rosales-Munar, Taylor H. H. Diaz-Herrera, Jose Usme Ciro, Edgar Parra, Diego A. Alvarez-Diaz, Katherine Laiton-Donato, Maria Leonor Caldas
Summary: This study presents the results of fragment sequencing assays on paraffin-embedded tissue samples from fatal DENV cases during the 2010 epidemic in Colombia. We found that the predominant serotype was DENV-2, with the Asian/American genotype of lineages 1 and 2.
Article
Microbiology
Ningning Yang, Mingguo Xu, Zhenguo Ma, Honghuan Li, Shengnan Song, Xiaoxiao Gu, Jingnan Liu, Zhonglian Yang, Hongji Zhu, Hailong Ma, Jihai Yi, Yong Wang, Zhen Wang, Jinliang Sheng, Chuangfu Chen
Summary: Xinjiang pastoral area, the second largest pastoral area in China, has a high prevalence rate of BVDV infection in dairy cows, with the genetic diversity increasing. HoBiPeV, an emerging atypical Pestivirus, was successfully identified and isolated in Xinjiang for the first time, posing a potential threat to the cattle industry in Xinjiang.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Luciano F. Huergo, Khaled A. Selim, Marcelo S. Conzentino, Edileusa C. M. Gerhardt, Adrian R. S. Santos, Berenike Wagner, Janette T. Alford, Nelli Deobald, Fabio O. Pedrosa, Emanuel M. de Souza, Meri B. Nogueira, Sonia M. Raboni, Denio Souto, Fabiane G. M. Rego, Dalila L. Zanette, Mateus N. Aoki, Jeanine M. Nardin, Bruna Fornazari, Hugo M. P. Morales, Vania A. Borges, Annika Nelde, Juliane S. Walz, Matthias Becker, Nicole Schneiderhan-Marra, Ulrich Rothbauer, Rodrigo A. Reis, Karl Forchhammer
Summary: The study developed a simple immunoassay method for rapid, inexpensive, and quantitative detection of human antibodies against SARS-CoV-2 in serum. The method showed comparable performance to traditional ELISA and Luminex assays but required less consumables, instrumentation, time, and sample volume.
Article
Parasitology
Larissa Staurengo-Ferrari, Raquel Arruda da Silva Sanfelice, Julia Bagatim de Souza, Joao Paulo Assolini, Dayane Priscila dos Santos, Allan Henrique Depieri Cataneo, Juliano Bordignon, Ivete Conchon-Costa, Idessania Nazareth da Costa, Glaura Scantamburlo Alves Fernandes
Summary: T. gondii infection can lead to an increase in the number of infected Leydig cells and affect the production of testosterone and inflammatory mediators, thus compromising male reproductive system.
Review
Microbiology
Allan Henrique Depieri Cataneo, Eloah Pereira Avila, Larissa Albuquerque de Oliveira Mendes, Viviane Guedes de Oliveira, Camila Rodrigues Ferraz, Mauro Vieira de Almeida, Sandra Frabasile, Claudia Nunes Duarte dos Santos, Waldiceu Aparecido Verri Jr, Juliano Bordignon, Pryscilla Fanini Wowk
Summary: Zika virus is mainly transmitted to humans by Aedes mosquitoes and can lead to serious consequences such as birth defects, microcephaly, and Guillain-Barre syndrome. Despite efforts, there are currently no effective vaccines or drugs to control Zika virus infection. Flavonoids, natural compounds with antiviral properties, are being explored as potential anti-Zika molecules for further research and clinical trials.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Developmental Biology
Juan C. Opazo, Federico G. Hoffmann, Kattina Zavala, Scott Edwards
Summary: The DAN gene family plays fundamental roles in development and is associated with diseases in adulthood. This study investigates the evolutionary history of the DAN gene family in vertebrates, identifying two new gene lineages and reconstructing the ancestral repertoire of DAN genes in gnathostomes.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael W. Vandewege, Roy N. I. I. I. I. Patt, Dana K. Merriman, David A. Ray, Federico G. Hoffmann
Summary: The PIWI/piRNA defense system against transposable elements (TEs) is less effective in the absence of actively mobilizing TEs. The study on the 13-lined ground squirrel reveals that while there is no active TE insertion, TEs are still actively transcribed. The Piwi genes are not expressed until after a certain stage of testis development, and there is little change in TE expression with the onset of Piwi expression. This suggests that the PIWI/piRNA system is less efficient in suppressing TE transcription in squirrels compared to mice and rabbits.
Article
Infectious Diseases
Ludmila A. Assis, Moezio V. C. Santos Filho, Joao R. da Cruz Silva, Maria J. R. Bezerra, Irassandra R. P. U. C. de Aquino, Kleison C. Merlo, Fabiola B. Holetz, Christian M. Probst, Antonio M. Rezende, Barbara Papadopoulou, Tamara D. C. da Costa Lima, Osvaldo P. de Melo Neto
Summary: Poly(A) Binding Proteins (PABPs) are key eukaryotic RNA-binding proteins with roles in mRNA stability and translation. Leishmania, a flagellated protozoan, has three distinct PABPs with preferential associations to different mRNAs encoding ribosomal proteins. This study identified specific RNA binding partners for each PABP, shedding light on their unique roles in mRNA translation regulation.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Infectious Diseases
Lucas Bochnia-Bueno, Sergio Monteiro De Almeida, Sonia Mara Raboni, Douglas Adamoski, Ludmilla Louise Moreira Amadeu, Suzana Carstensen, Meri Bordignon Nogueira
Summary: This study aimed to evaluate the immunogenicity of the CoronaVac vaccine in healthcare workers. The results showed a high seroconversion rate of the vaccine in this population.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Correction
Immunology
Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, Taylon Felipe Silva, Bruna Taciane da Silva Bortoleti, Manoela Daiele Goncalves, Virginia Marcia Concato, Ana Carolina Jacob Rodrigues, Mariana Barbosa Detoni, Larissa Staurengo-Ferrari, Waldiceu Aparecido Verri, Idessania Nazareth Costa, Carolina Panis, Ivete Conchon-Costa, Juliano Bordignon, Wander Rogerio Pavanelli
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Virology
Ana Paula Andreolla, Alessandra Abel Borges, Juliano Bordignon, Claudia Nunes Duarte dos Santos
Summary: Mayaro virus is a newly emerged arbovirus that causes similar symptoms to Chikungunya virus. While outbreaks of Mayaro virus mainly occur in northern and central-western states of Brazil, it has been spreading to other regions in recent years. Due to the similarity of initial symptoms with other endemic arboviruses, cases of Mayaro virus infection might be underestimated. The lack of specific prophylactic approaches or antiviral drugs for Mayaro virus limits the management of patients to relieving symptoms like pain and inflammation, similar to other pathogenic alphaviruses. This review aims to present the latest research progress on screening and developing compounds/molecules with potential anti-Mayaro virus activity and infection inhibition.
Article
Evolutionary Biology
William Joyce, Daniel M. Ripley, Todd Gillis, Amanda Coward Black, Holly A. Shiels, Federico G. Hoffmann
Summary: The troponin complex, responsible for muscle activation, is composed of three interacting protein subunits: TnC, TnI, and TnT. Through phylogenetic and synteny analysis, we have identified five distinct TNNI classes (TNNI1-5) and four TNNT classes (TNNT1-4) in a diverse array of vertebrates.
GENOME BIOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Juan C. Opazo, Michael W. Vandewege, Federico G. Hoffmann, Kattina Zavala, Catalina Melendez, Charlotte Luchsinger, Viviana A. Cavieres, Luis Vargas-Chacoff, Francisco J. Morera, Patricia Burgos, Cheril Tapia-Rojas, Gonzalo A. Mardones
Summary: Studying the evolutionary history of gene families is important for understanding gene origin, evolution, and their implications for functional studies. The sirtuin gene family, involved in aging-related functions, has a complex duplicative history, but our results provide a well-resolved phylogeny and shed light on the repertoire of sirtuin genes among vertebrates. We identified a new member (SIRT3.2) that was lost in amniotes but retained in other jawed vertebrates, and its experimental analysis revealed its mitochondrial location and deacetylase activity similar to mammalian SIRT3.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Yingpu Yu, William M. Schneider, Maximilian A. Kass, Eleftherios Michailidis, Ashley Acevedo, Ana L. Pamplona Mosimann, Juliano Bordignon, Alexander Koenig, Christine M. Livingston, Hardeep van Gijzel, Yi Ni, Pradeep M. Ambrose, Catherine A. Freije, Mengyin Zhang, Chenhui Zou, Mohammad Kabbani, Corrine Quirk, Cyprien Jahan, Xianfang Wu, Stephan Urban, Shihyun You, Amir Shlomai, Ype P. de Jong, Charles M. Rice
Summary: Hepatitis B virus (HBV) is a chronic infection affecting approximately 300 million people, with current treatments being ineffective. The virus increases the risk of liver cirrhosis and hepatocellular carcinoma, causing nearly 1 million deaths annually. A new method using synthetic RNA to initiate HBV replication is described, eliminating background signals from input virus or plasmid DNA. This approach can be used to study multiple stages of HBV replication and identify sequence variants that confer resistance to antiviral drugs.
Article
Cell Biology
Carina Z. Segato-Vendrameto, Camila Zanluca, Amanda Z. Zucoloto, Tiago H. Zaninelli, Mariana M. Bertozzi, Telma Saraiva-Santos, Camila R. Ferraz, Larissa Staurengo-Ferrari, Stephanie Badaro-Garcia, Marilia F. Manchope, Amanda M. Dionisio, Felipe A. Pinho-Ribeiro, Sergio M. Borghi, Ana Luiza Pamplona Mosimann, Rubia Casagrande, Juliano Bordignon, Victor Fattori, Claudia N. Duarte dos Santos, Waldiceu A. Verri
Summary: This article investigates therapeutic strategies against Chikungunya virus, finding that inhibiting viral proteins and using recombinant antibodies can alleviate virus-induced pain symptoms.
Article
Parasitology
Christian Macagnan Probst, Myllena de Fatima Alheiros Dias Melo, Daniela Parada Pavoni, Max Jean de Ornelas Toledo, Tainah Silva Galdino, Adeilton Alves Brandao, Constanca Britto, Marco Aurelio Krieger
Summary: A molecular method for T. cruzi genotyping based on PCR amplification of a single target with multiple copies in the nuclear genome by large scale sequencing was developed. This method classified all samples into sub DTU level and determined the evolutionary relationship between DTUs, suggesting that TcIII and TcIV DTUs are non-hybrid and DTU IV is more similar to the common ancestral. As the TS-LSS method is based on a single PCR reaction with several copies of the target, it is potentially useful for clinical samples and can be increasingly applied for T. cruzi genotyping as large scale sequencing systems become more common.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2021)
