Journal
MEDICINAL CHEMISTRY RESEARCH
Volume 19, Issue 8, Pages 948-961Publisher
SPRINGER BIRKHAUSER
DOI: 10.1007/s00044-009-9241-5
Keywords
alpha-Glucosidase inhibitor; alpha-Amylase inhibitor; Gymnema montanum; Type 2 diabetes; nSTZ rats; Medicinal plants
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Funding
- Council of Scientific and Industrial Research, New Delhi
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The present study was attempted to investigate the effect of G. montanum leaf extract on inhibition of alpha-glucosidase and alpha-amylase activity. The ethanol extract of G. montanum (GLEt) at various concentrations (1-10 mu g/ml) was tested for its inhibition pattern against alpha-glucosidase and alpha-amylase activity in vitro and compared with the commercially available alpha-glucosidase inhibitor, acarbose. The GLEt showed competitive inhibition against yeast alpha-glucosidase and noncompetitive inhibition against salivary alpha-amylase in a concentration-dependent manner. Further, we investigated the effect of extract on levels of blood glucose and plasma insulin in neonatal streptozotocin-induced type 2 diabetic rats. Long-term administration (12 weeks) of the GLEt effectively reduced the blood glucose level and also increased the insulin level. In a preliminary phytochemical analysis, G. montanum leaves were rich in phenolic composition and found to be positively correlated with the inhibitory effect of alpha-glucosidase and alpha-amylase activity. These results suggest that the GLEt might exert its antidiabetic effect by suppressing carbohydrate absorption from the intestine and thereby reducing hyperglycemia.
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