Article
Cell Biology
Ludmila Matos Baltazar, Gabriela Fior Ribeiro, Gustavo J. Freitas, Celso Martins Queiroz-Junior, Caio Tavares Fagundes, Carlos Chaves-Olortegui, Mauro Martins Teixeira, Daniele G. Souza
Summary: Paracoccidioidomycosis is a systemic disease endemic in Latin America, with most cases registered in Brazil. Research has been conducted to improve treatment effectiveness and protection against the disease, including the investigation of extracellular vesicles as potential antigen delivery vehicles.
Article
Microbiology
Caroline Maria Marcos, Haroldo Cesar de Oliveira, Patricia Akemi Assato, Lariane Teodoro de Oliveira, Nathalia Fregonezi, Kelvin Sousa dos Santos, Caroline Barcelos Costa-Orlandi, Ana Marisa Fusco-Almeida, Maria Jose Soares Mendes-Giannini
Summary: This study explores signaling pathways implicated in fungal pathobiology and identifies the crucial role of the Drk1 protein in the morphological shift and virulence of Paracoccidioides brasiliensis. Peptides with affinity for PbDrk1 were found to inhibit the phase transition and adhesion of P. brasiliensis, and augment the effects of antifungal agents. The peptides also showed enhanced larval survival rates in a Galleria mellonella model. Further investigation of PbDrk1 may improve our understanding of P. brasiliensis and lead to the development of novel therapeutic strategies.
Review
Microbiology
Gernot Wagner, Deddo Moertl, Anna Glechner, Verena Mayr, Irma Klerings, Casey Zachariah, Miriam Van den Nest, Gerald Gartlehner, Birgit Willinger
Summary: Paracoccidioidomycosis is a systemic mycosis endemic to Central and South America, with cases imported to Europe through migration and travel. A systematic review identified 83 cases of paracoccidioidomycosis diagnosed in 11 European countries, highlighting challenges in diagnosing the disease in individuals returning or immigrating from endemic areas, given the varied age of patients and time of symptom onset.
Article
Mycology
Anderson Messias Rodrigues, Ferry Hagen, Rosana Puccia, Rosane Christine Hahn, Zoilo Pires de Camargo
Summary: Paracoccidioidomycosis (PCM) is a group of diseases caused by Paracoccidioides species. The genus has expanded to include seven species. Classic PCM affects the human lungs and may lead to systemic granulomatous disease, while PCM loboi and PCM ceti are observed as subcutaneous mycoses in humans and dolphins. Recognizing species boundaries in Paracoccidioides is crucial for understanding its ecology, evolution, clinical features, and strategies to mitigate PCM.
Article
Microbiology
Luciana Bonome Zeminian de Oliveira, Amanda Manoel Della Coletta, Taiane Priscila Gardizani, Hans Garcia Garces, Eduardo Bagagli, Luciana Trilles, Ligia Vizeu Barrozo, Silvio de Alencar Marques, Julio De Faveri, Luciane Alarcao Dias-Melicio
Summary: Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America. Recent studies have identified three cryptic species in addition to Paracoccidioides brasiliensis. Through analysis of biopsy samples from patients, it has been found that P. brasiliensis is the predominant species in the Sao Paulo State region.
Article
Immunology
Aparecido Ferreira de Souza, Laurine Lacerda Pigosso, Lana O'Hara Souza Silva, Italo Dany Cavalcante Galo, Juliano Domiraci Paccez, Kleber Santiago Freitas e Silva, Milton Adriano Pelli de Oliveira, Maristela Pereira, Celia Maria de Almeida Soares
Summary: In this study, the exoproteome of Paracoccidioides brasiliensis (Pb18) under iron deprivation conditions was analyzed using nanoUPLC-MSE. One hundred forty-one proteins were identified, out of which 64 were predicted to be secreted. The regulation of several virulence factors was also identified, and Cyb5 was highlighted as a secreted molecule potentially involved in iron homeostasis in P. brasiliensis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Andreia Ferreira Nery, Zoilo Pires de Camargo, Anderson Messias Rodrigues, Tiago Ferreira Portela, Hugo Dias Hoffmann-Santos, Breno Goncalves Pinheiro, Ana Paula Possa, Leticia Rossetto da Silva Cavalcante, Ferry Hagen, Rosane Christine Hahn
Summary: This study investigated the clinical and laboratory data of 44 paracoccidioidomycosis patients caused by Paracoccidioides lutzii. The neutrophil to lymphocyte ratio (NLR) was found to complement the criteria for disease severity, and applying the low-cost NLR test can greatly increase diagnostic sensitivity and contribute to better disease control. Treatment prescribed did not follow recommendations in some severe cases.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Mycology
Giannina Ricci, Emeline Boni Campanini, Angela Satie Nishikaku, Rosana Puccia, Mariangela Marques, Ralf Bialek, Anderson Messias Rodrigues, Wagner Luiz Batista
Summary: This study identified six phylogenetic lineages belonging to the P. brasiliensis complex, with two lineages not grouping with any of the four recognized species and a coinfection involving two lineages. Additionally, the study found five parsimony-informative sites with potential non-synonymous substitutions that could lead to changes in the protein.
Article
Immunology
Juliana S. de Curcio, Lucas Nojosa Oliveira, Mariana P. Batista, Evandro Novaes, Celia Maria de Almeida Soares
Summary: Research shows that under iron deprivation conditions, Paracoccidioides brasiliensis produces a variety of microRNAs, which may regulate metabolic pathways related to iron deficiency.
MICROBES AND INFECTION
(2021)
Review
Microbiology
Paulo Mendes Pecanha, Paula Massaroni Pecanha-Pietrobom, Tania Regina Grao-Velloso, Marcos Rosa Junior, Aloisio Falqueto, Sarah Santos Goncalves
Summary: Paracoccidioidomycosis is a systemic mycosis endemic to Latin America. The disease is usually acquired in rural areas but symptoms may occur years later. In Brazil, the incidence is rising in the north and decreasing in the south. Diagnostic methods are scarce, mainly relying on direct microscopic examination.
Article
Plant Sciences
Lauana Aparecida Santos, Pedro Luiz Rosalen, Nayara Andrade Dias, Julianne Caravita Grisolia, Bruno Jose Nascimento Gomes, Lucas Blosfeld-Lopes, Masaharu Ikegaki, Severino Matias de Alencar, Eva Burger
Summary: Brazilian Red propolis shows direct antifungal activity against Paracoccidioides brasiliensis, activates neutrophils, prevents fungal dissemination, and controls excessive inflammation.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Microbiology
Adriane Lenhard-Vidal, Raquel Bender, Joao Paulo Assolini, Ana Elisa Mayumi Maruo, Luiz Tiago Vieira, Ellen Cristina Pereira, Mario Augusto Ono, Ayako Sano, Eiko Nakagawa Itano
Summary: This study aimed to assess the infection rate of paracoccidioidomycosis in an inland municipality of southern Brazil, revealing the epidemiological profile of the disease in the region, as well as the potential of CFA-ELISA for serological screening in epidemiological surveys.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Stephan Alberto Machado de Oliveira, Janayna Nunes Reis, Elisa Catao, Andre Correa Amaral, Ana Camila Oliveira Souza, Alice Melo Ribeiro, Lucia Helena Faccioli, Fabiana Pirani Carneiro, Clara Luna Freitas Marina, Pedro Henrique Burgel, Larissa Fernandes, Aldo Henrique Tavares, Anamelia Lorenzetti Bocca
Summary: The results of the study indicate that CD18(low) mice infected with Pb18 survived with fewer granulomas, lower fungal burden, lower nitrate levels, and high IgG1 levels compared to WT mice. In addition, the in vitro macrophages from CD18(low) mice showed slower internalization of yeast cells and lower fungal burden, suggesting that beta 2 integrins play a crucial role in fungal survival within macrophages.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Lucas Nojosa Oliveira, Patricia de Sousa Lima, Danielle Silva Araujo, Igor Godinho Portis, Agenor de Castro Moreira dos Santos Junior, Alexandre Siqueira Guedes Coelho, Marcelo Valle de Sousa, Carlos Andre Ornelas Ricart, Wagner Fontes, Celia Maria de Almeida Soares
Summary: Paracoccidioides brasiliensis reprograms its metabolism under hypoxia to reduce basal metabolism, increase energy and general metabolism, and modify glucose utilization for glycolysis or cell wall synthesis. Changes in plasma membrane/cell wall composition and activation of detoxification mechanisms are also observed. Proteomic analysis revealed up-regulation of biochemical pathways related to acetyl-CoA, pyruvate, and ergosterol synthesis.Metabolic adaptation processes differ between Paracoccidioides species, with distinct proteome profiles in response to hypoxia.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Dermatology
Igor Massahiro Souza de Suguiura, Mario Augusto Ono
Summary: Paracoccidioidomycosis is an important endemic mycosis in the state of Parana, with reported cases mainly coming from specialized health units and the western region showing the highest incidence. Despite a decreasing trend in confirmed cases, the disease still has the highest mortality rate compared to other mycoses. This study provides an epidemiological baseline for future surveillance and modifications of paracoccidioidomycosis in the region.
Article
Infectious Diseases
Franciane Rosa Miranda, Kenner Morais Fernandes, Cliver Fernandes Farder-Gomes, Rodrigo Cupertino Bernardes, Andre Henrique de Oliveira, Mayara Badaro Arthidoro de Castro, Lidia Aparecida Dourado, Leandro Licursi Oliveira, Gustavo Ferreira Martins, Jose Eduardo Serrao
Summary: Copper sulfate has insecticidal activity against Aedes aegypti, possibly through affecting midgut metamorphosis and reducing proliferation of stem cells, resulting in impaired female mosquito fertility and fecundity.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Agriculture, Dairy & Animal Science
Maykelly da S. Gomes, Alysson Saraiva, Dante T. Valente Junior, Leandro L. de Oliveira, Amanda M. Correia, Nicola V. L. Serao, Gabriel C. Rocha
Summary: This study showed that supplementing arginine + glutamine in pig diets during the growing phase improved growth performance and immune response compared to diets containing antibiotics, but had no significant impact on antioxidant capacity. Antibiotics improved growth performance only during the treatment phase, with no post-treatment effect.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Plant Sciences
Cristiane A. Milagres, Renata Belisario, Daiana M. Q. Azevedo, Leandro L. Oliveira, Fabricio A. Rodrigues, Gleiber Q. Furtado
Summary: This study investigated the infection process of Pseudobeltrania cedrelae on leaves of Cedrelae fissilis and the possible role of Phyllachora balansae in the development of pseudobeltrania spot. The results showed that a higher number of germinated P. cedrelae conidia and appressoria were observed in samples containing P. balansae, whereas conidia barely germinated on healthy leaflets without P. balansae. The penetration of P. cedrelae into the leaflets occurred through both direct penetration and stomata. P. balansae plays an important role in the development of pseudobeltrabia spot.
JOURNAL OF PHYTOPATHOLOGY
(2022)
Article
Food Science & Technology
Leandro de Souza Lopes, Marliane de Casssia Silva, Alessandra de Oliveira Faustino, Leandro Licursi de Oliveira, Maria Catarina Megumi Kasuya
Summary: The study found that different mushroom species have varying sensitivity to high concentrations of lithium, with enriched mushrooms showing increased mineral co-accumulation and oxidative activity. This confirms that lithium-enriched mushrooms can be a healthy alternative source of lithium.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Oncology
Soledad Palameta, Andrea J. Manrique-Rincon, Jessica M. Toscaro, Isadora F. Semionatto, Matheus C. Fonseca, Rhubia S. M. Rosa, Luciana P. Ruas, Paulo S. L. Oliveira, Marcio C. Bajgelman
Summary: Therapeutic strategies based on immunomodulation have improved cancer therapy. A novel platform using virus-like particles derived from lentiviral capsids has been proposed to deliver targeted immunomodulatory signaling, enhancing antitumor response. The platform can be engineered to possess multifunctional ligands on the surface, driving tropism to the tumor site and delivering immunomodulatory signaling.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Medicine, Research & Experimental
Juliana Alves do Vale, Michelle Peixoto Rodrigues, Angela Maria Almeida Lima, Samira Soares Santiago, Graziela Domingues de Almeida Lima, Alisson Andrade Almeida, Leandro Licursi de Oliveira, Gustavo Costa Bressan, Robson Ricardo Teixeira, Mariana Machado-Neves
Summary: Melanoma, the most aggressive skin cancer, has been increasing in incidence over the past decades. Conventional treatments have severe side effects and melanoma can be resistant to commonly used anticancer drugs, necessitating the search for new potential anti-melanoma drugs. In this study, we synthesized seventeen compounds derived from cinnamic acid and evaluated their bioactivity against melanoma cells. Phenyl 2,3-dibromo-3-phenylpropanoate (3q) was found to be the most effective compound against murine B16-F10 cells, showing cytotoxicity and inhibitory effects on cell migration. It also had low cytotoxicity on non-tumor cells. Additionally, compound 3q induced apoptosis at high concentrations, while affecting cell cycle and melanoma cell proliferation at lower concentrations. Furthermore, cinnamate 3q inhibited cell invasion, adhesion, colonization, and actin polymerization. These results demonstrate the antiproliferative and antimetastatic potential of cinnamic acid derivatives on melanoma.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Nutrition & Dietetics
Andressa Ladeira Bernardes, Luis Fernando de Sousa Moraes, Bruna Cristina dos Santos Cruz, Lisiane Lopes da Conceicao, Leandro Licursi de Oliveira, Mariaurea Matias Sarandy, Reggiani Vilela Goncalves, Maria do Carmo Gouveia Peluzio
Summary: This study investigates the effects of supplementation with 5% and 10% dehydrated H. sabdariffa calyces (DHSC) on the development of pre-neoplastic lesions in mice. The results show that DHSC supplementation can reduce the formation of aberrant crypt foci (ACF) and have positive effects on liver enzyme activity and colon mucosa inflammation.
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Nutrition & Dietetics
Fabricia Geralda Ferreira, Luiza Kuhnen Reitz, Aline Valmorbida, Mariana Papini Gabiatti, Fernanda Hansen, Patricia Faria Di Pietro, Leandro Licursi de Oliveira, Erasmo Benicio Santos de Moraes Trindade, Giana Zarbato Longo
Summary: The study aimed to investigate the association between cytokine levels and metabolic phenotypes, and the results showed that an unhealthy metabolic profile is associated with higher levels of proinflammatory cytokines. Excess weight and metabolic dysregulation are related to inflammation, especially when both conditions are present, possibly due to visceral adiposity.
Article
Nutrition & Dietetics
Aline Valmorbida, Giana Zarbato Longo, Giovanna Mozzaquattro Nascimento, Leandro Licursi de Oliveira, Erasmo Benicio Santos de Moraes Trindade
Summary: Obesity is associated with chronic low-grade inflammation. This study found significant associations between anthropometric measurements and inflammatory cytokines. The results highlight the importance of anthropometric assessment in clinical practice and the need for public policies to reduce obesity, inflammation, and metabolic complications.
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Nutrition & Dietetics
Juliana Farias de Novaes, Mariana De Santis Filgueiras, Lara Gomes Suhett, Mariane Alves Silva, Fernanda Martins de Albuquerque, Erica Priulli, Leandro Licursi de Oliveira, Maria do Carmo Gouveia Peluzio
Summary: The study aimed to evaluate the association between body fat, anti- and inflammatory adipokines, and anti- and oxidative markers. A cross-sectional study of 378 schoolchildren in Minas Gerais, Brazil, was conducted. Body fat was estimated using dual energy x-ray absorptiometry, and blood samples were collected to analyze adipokines and antioxidant markers. Results showed that total and central body fat were positively associated with ferric reducing antioxidant power (FRAP), while adiponectin (an anti-inflammatory marker) was inversely associated with FRAP. Chemerin (an adiposity-related inflammation marker) was positively associated with superoxide dismutase (SOD).
Article
Plant Sciences
Greice Lubini, Pedro Boscariol Ferreira, Andrea Carla Quiapim, Michael Santos Brito, Viviane Cossalter, Maria Cristina S. Pranchevicius, Maria Helena S. Goldman
Summary: Successful plant reproduction and fruit formation rely on pollen and pistil development, as well as interactions between them. In this study, we focused on Nicotiana tabacum and investigated the role of a gene called NtPAE1 in the modulation of pectin esterification, which is crucial for the formation of intercellular spaces in pistil-specialized tissues. We found that NtPAE1 is preferentially expressed in stigmas/styles and ovaries, with high expression in the stylar transmitting tissue. Through transgenic plant experiments, we demonstrated that NtPAE1 is essential for normal flower development and pollen tube growth, ultimately affecting fruit production.
Article
Entomology
Barbara Cristina Felix Nogueira, Andres Mauricio Ortega Orozco, Ana Karina Argumedo, Alessandra de Oliveira Faustino, Leandro Licursi de Oliveira, Leandro Abreu da Fonseca, Artur Kanadani Campos
Summary: This study aimed to evaluate the concentration of acute phase proteins and circulating oxidative stress in response to tick infestation in two breed horses. The results showed that tick infestation had some impact on oxidative stress markers, but no significant changes were observed in most of the analyzed variables. Further studies are needed to understand the compounds and mechanisms of tick saliva in acute phase proteins and the relationship between oxidative stress in the host and the tick during blood feeding.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2023)
Article
Food Science & Technology
Mariana de Moura e Dias, Vinicius da Silva Duarte, Lucio Flavio Macedo Mota, Gabriela de Cassia Avila Alpino, Sandra Aparecida dos Reis Louzano, Lisiane Lopes da Conceicao, Hilario Cuquetto Mantovanie, Solange Silveira Pereira, Leandro Licursi Oliveira, Tiago Antonio de Oliveira Mendes, Davide Porcellato, Maria do Carmo Gouveia Peluzio
Summary: This study examines the role of probiotics, antimicrobials, and diet in modulating gut microbiota and improving intestinal health. The results show that high-fat diet reduces bacterial diversity and richness in the gut, but these effects can be counteracted with probiotics and a specific diet, leading to improved intestinal health.
Article
Biotechnology & Applied Microbiology
Leandro de Souza Lopes, Marliane de Cassia Soares da Silva, Juliana Soares da Silva, Jose Maria Rodrigues da Luz, Alessandra de Oliveira Faustino, Gabriel Cipriano Rocha, Leandro Licursi de Oliveira, Maria Catarina Megumi Kasuya
Summary: This study evaluated the bioavailability of lithium-enriched Pleurotus djamor mushrooms in pigs and their effects on oxidative stress. The results showed that these mushrooms are a more bioavailable source of lithium and have protective effects against oxidative stress in animal tissues.
Article
Biochemistry & Molecular Biology
Mariana de Fatima Albuquerque Pereira, Larissa Gabriela Morais de Avila, Bruna Cristina dos Santos Cruz, Bruno Campos Silva, Leandro Licursi de Oliveira, Reggiani Vilela Goncalves, Andrea de Oliveira Barros Ribon, Tiago Antonio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio
Summary: This study evaluated the role of milk kefir on inflammation and gut microbiome in mice infected with Salmonella. The results showed that kefir could prevent systemic infections and regulate cytokines in IL-10(-/-) mice, but not in wild type mice. These findings demonstrate the importance of IL-10 in the prognosis of salmonellosis and the potential use of milk kefir in acute infections.
