Article
Multidisciplinary Sciences
Tinmaya Rai, Srijan Shrestha, Sabita Prajapati, Anup Bastola, Niraj Parajuli, Pragya Gautam Ghimire, Parmananda Bhandari, Kishor Pandey, Manju Jain, Greg Matlashewski, Rachel Bras-Goncalves, Krishna Das Manandhar
Summary: Cutaneous leishmaniasis cases have increased significantly in Nepal. This study identified the Leishmania species in 40 patient samples using molecular methods. The results showed the presence of L. donovani complex and L. major as the causative agents. Moreover, the study found the concurrent occurrence of atypical and typical cutaneous infections caused by these two species.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Li Wang, Tao Yu, Yaxin Zhu, Yingfeng Luo, Fan Dong, Xuemei Lin, Wenzhong Zhao, Zilong He, Songnian Hu, Zhiyang Dong
Summary: This study demonstrates that microbial networks on the scalp and hair surface of dandruff patients are less stable compared to their healthy counterparts, with lower node degree and more positive and stronger links. Lactobacillus bacteria have extensive interactions with other bacteria or fungi on the scalp and hair surface.
Article
Multidisciplinary Sciences
Mincai Nie, Yuancheng Zhou, Fengqin Li, Huidan Deng, Mengxi Zhao, Yao Huang, Chaoyuan Jiang, Xiangang Sun, Zhiwen Xu, Ling Zhu
Summary: This article presents a rapid detection method for studying the epidemiology of JEV in pigs in Sichuan province, and finds a high prevalence of JEV GI strains in the area.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Hao T. Mai, Brice C. Vanness, Thomas H. Linz
Summary: This report describes the development of a method to rapidly quantify miRNA in digital microwell arrays using base-stacking digital-quantitative-PCR (BS-dqPCR). BS-dqPCR expedites miRNA measurements by eliminating the need for ligation and reverse transcription steps, which reduces the time and cost compared to conventional miRNA PCR analyses.
Article
Chemistry, Analytical
Wan-chen Tu, Anika M. McManamen, Xiaojing Su, Ingrid Jeacopello, Meg G. Takezawa, Damielle L. Hieber, Grant W. Hassan, Ulri N. Lee, Eden V. Anana, Mason P. Locknane, Molly W. Stephenson, Victoria A. M. Shinkawa, Ellen R. Wald, Gregory P. DeMuri, Karen N. Adams, Erwin Berthier, Sanitta Thongpang, Ashleigh B. Theberge
Summary: Researchers conducted the first study on human subjects using the CandyCollect device, a lollipop-inspired saliva collection device, and successfully collected salivary bacteria from healthy adults. The results showed a 100% concordance between CandyCollect devices and traditional sampling methods in detecting bacteria. The CandyCollect device was ranked as the preferred sampling method and has a shelf life of up to 1 year at room temperature, making it convenient for clinic visits and telemedicine.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Rituja Saxena, Parul Mittal, Cecile Clavaud, Darshan B. Dhakan, Nita Roy, Lionel Breton, Namita Misra, Vineet K. Sharma
Summary: Dandruff is a common scalp disorder, and topical application of coconut oil can enrich healthy scalp-related bacterial pathways and decrease fungal pathogenesis pathways, helping to maintain a healthy scalp.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Max Grogl, Christie Joya, Maria Saenz, Ana Quispe, Luis Angel Rosales, Rocio del Pilar Santos, Maxy de los Santos, Ngami Donovan, Janet Ransom, Ana Ramos, Elmer Llanos Cuentas
Summary: The primary objective of this study was to determine the sensitivity and specificity of the FDA-cleared CL Detect Rapid Test in Peru. The study found that the modified test procedures, including increasing extraction time and sample volume, resulted in higher sensitivity for specimens obtained by scraping compared to those from dental broach. Molecular analysis also showed that the modified CL Detect was more sensitive than microscopy.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Immunology
Luma Al-Bayati, Bahar Nayeri Fasaei, Shahin Merat, Alireza Bahonar, Arefeh Ghoddusi
Summary: This study quantified the abundance of F. prausnitzii, Provetella, and Peptostreptococcus in UC and non-UC patients using Real-Time PCR. The results showed decreased abundance of these bacterial populations in the intestine of UC patients compared to non-UC patients.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Giulia Pivetta, Ludovica Dottori, Federico Fontana, Sophia Cingolani, Irene Ligato, Emanuele Dilaghi, Christian Milani, Marco Ventura, Marina Borro, Gianluca Esposito, Bruno Annibale, Edith Lahner
Summary: This study aimed to assess gender differences in gastric bacterial microbiota between subjects with healthy stomachs and those with autoimmune atrophic gastritis. Results showed that women with healthy stomachs had a higher gastric bacterial abundance and less microbial diversity compared to men. Autoimmune atrophic gastritis seems to reset gender differences in gastric bacterial abundance and reduce biodiversity in males.
Article
Food Science & Technology
Seul-Ah Kim, Dong Hyeon Lee, Byung Hee Ryu, Nam Soo Han
Summary: A newly developed PCR method was utilized to rapidly and accurately monitor the population changes of L. mesenteroides DRC1506 during kimchi fermentation, providing an effective means for quality control in the fermentation process.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Dermatology
Yoojeong Choi, Kui Young Park, Hye Sung Han, Mi-Kyung Lee, Seong Jun Seo
Summary: This study comparatively analyzed the cutaneous fungal distribution between AD patients and healthy individuals. The results showed that the distribution of Candida spp., dermatophytes, and Malassezia spp. were altered with AD development.
ANNALS OF DERMATOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Mathijs J. P. Theelen, Roosmarijn E. C. Luiken, Jaap A. Wagenaar, Marianne M. Sloet Van Oldruitenborgh-Oosterbaan, John W. A. Rossen, Aldert L. Zomer
Summary: Several studies have described the faecal microbiota of horses and factors influencing its composition, but with substantial variation in results. The current study in The Netherlands investigated the microbiota composition in healthy horses and ponies under standard conditions, finding that factors such as location, age, season, horse type, and pasture access significantly affect the composition. This research provides important baseline information for future studies on equine faecal microbiota composition and its influencing factors.
Article
Multidisciplinary Sciences
Pritam Sukul, Simon Grzegorzewski, Celine Broderius, Phillip Trefz, Thomas Mittlmeier, Dagmar-Christiane Fischer, Wolfram Miekisch, Jochen K. Schubert
Summary: The study analyzed exhaled volatile metabolites data from 204 healthy females to investigate the age-related substance-specific differences and their relationship with endocrine differences, energy homeostasis, microbial diversity, oxidative stress, and lifestyle. The results suggest that breathomics offers a noninvasive approach for understanding aging at a systemic level and personalized monitoring.
Article
Immunology
Abigail J. Porzucek, Abigale M. Proctor, Katharina E. Klinkhammer, Sarah R. Tritsch, Molly A. Robertson, Jonathan P. Bashor, Jack Villani, Jorge L. Sepulveda, Christopher N. Mores
Summary: During the 2022 monkeypox outbreak, limited testing and delayed results allowed for ongoing transmission of the virus. This research adapted and validated a qPCR assay for monkeypox detection, which showed high sensitivity and specificity. Broad implementation of this assay in research and public health laboratories can help increase diagnostic capacity amid the growing outbreak.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Microbiology
Jonguk Park, Kumiko Kato, Haruka Murakami, Koji Hosomi, Kumpei Tanisawa, Takashi Nakagata, Harumi Ohno, Kana Konishi, Hitoshi Kawashima, Yi-An Chen, Attayeb Mohsen, Jin-zhong Xiao, Toshitaka Odamaki, Jun Kunisawa, Kenji Mizuguchi, Motohiko Miyachi
Summary: This study conducted a comprehensive analysis of gut microbiota in healthy Japanese individuals based on two independent cohorts, revealing that questionnaire-based covariates such as frequency of bowel movement and specific dietary habits impact the microbial composition of the gut. This was the first study to investigate integrated and non-redundant factors associated with gut microbiota among Japanese populations.
