4.7 Article Proceedings Paper

Cell segmentation in phase contrast microscopy images via semi-supervised classification over optics-related features

Journal

MEDICAL IMAGE ANALYSIS
Volume 17, Issue 7, Pages 746-765

Publisher

ELSEVIER
DOI: 10.1016/j.media.2013.04.004

Keywords

Phase contrast microscopy image; Sparse representation; Phase retardation feature; Semi-supervised classification; Cell segmentation

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Phase-contrast microscopy is one of the most common and convenient imaging modalities to observe long-term multi-cellular processes, which generates images by the interference of lights passing through transparent specimens and background medium with different retarded phases. Despite many years of study, computer-aided phase contrast microscopy analysis on cell behavior is challenged by image qualities and artifacts caused by phase contrast optics. Addressing the unsolved challenges, the authors propose (1) a phase contrast microscopy image restoration method that produces phase retardation features, which are intrinsic features of phase contrast microscopy, and (2) a semi-supervised learning based algorithm for cell segmentation, which is a fundamental task for various cell behavior analysis. Specifically, the image formation process of phase contrast microscopy images is first computationally modeled with a dictionary of diffraction patterns; as a result, each pixel of a phase contrast microscopy image is represented by a linear combination of the bases, which we call phase retardation features. Images are then partitioned into phase-homogeneous atoms by clustering neighboring pixels with similar phase retardation features. Consequently, cell segmentation is performed via a semi-supervised classification technique over the phase-homogeneous atoms. Experiments demonstrate that the proposed approach produces quality segmentation of individual cells and outperforms previous approaches. (C) 2013 Elsevier B.V. All rights reserved.

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