Article
Parasitology
Mahamat Hissene Mahamat, Adeline Segard, Jean-Baptiste Rayaisse, Rafael Argiles-Herrero, Andrew Gordon Parker, Philippe Solano, Adly Mohamed Mohamed Abd-Alla, Jeremy Bouyer, Sophie Ravel
Summary: Based on experimental data, the research found that sterile male tsetse flies cannot transmit Trypanosoma brucei brucei. The reliable evidence indicates that the release of large numbers of sterile male flies does not increase the potential risk of tsetse flies transmitting the parasite.
PARASITES & VECTORS
(2023)
Review
Parasitology
Shauna Richards, Liam J. Morrison, Steve J. Torr, Michael P. Barrett, Oliver Manangwa, Furaha Mramba, Harriet Auty
Summary: Trypanocides are crucial in controlling African animal trypanosomiasis in tsetse-infested areas, but issues such as inappropriate use, drug quality problems, and treatment failure have been highlighted by recent research. There are gaps in knowledge and investment in inexpensive AAT diagnostics, understanding of drug resistance, and effective use of trypanocides, making it difficult to develop best practices and policies for existing drugs or inform the development and use of new drugs. Understanding the drivers and behavioral practices around trypanocide use is important for sustainable solutions needed for effective control of AAT.
TRENDS IN PARASITOLOGY
(2021)
Article
Multidisciplinary Sciences
Mouhamadou M. Dieng, Kiswend-sida M. Dera, Percy Moyaba, Gisele M. S. Ouedraogo, Guler Demirbas-Uzel, Fabian Gstottenmayer, Fernando C. Mulandane, Luis Neves, Sihle Mdluli, Jean-Baptiste Rayaisse, Adrien M. G. Belem, Soumaila Pagabeleguem, Chantel J. de Beer, Andrew G. Parker, Jan Van den Abbeele, Robert L. Mach, Marc J. B. Vreysen, Adly M. M. Abd-Alla
Summary: The sterile insect technique is an effective and sustainable method for managing insect pests. This study examines the interaction between Sodalis bacteria and Trypanosoma infection in tsetse flies. The presence of Sodalis and Trypanosoma varied among different countries and tsetse species. The study also found differences in the prevalence of specific Trypanosoma species in different regions.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Sebastian Shaw, Sebastian Knuesel, Daniel Abbuehl, Arunasalam Naguleswaran, Ruth Etzensperger, Mattias Benninger, Isabel Roditi
Summary: African trypanosomes exhibit social motility on semi-solid surfaces. The authors found that procyclic forms acidify their environment due to glucose metabolism, generating pH gradients that are sensed via cyclic AMP signaling. Parasite mutants defective in cAMP signaling are inhibited in fly infection.
NATURE COMMUNICATIONS
(2022)
Article
Mathematics
Luis Almeida, Michel Duprez, Yannick Privat, Nicolas Vauchelet
Summary: This article studies the optimal release strategies for controlling disease transmission by using the sterile insect technique. Numerical simulations are presented to illustrate the results.
JOURNAL OF DIFFERENTIAL EQUATIONS
(2022)
Article
Parasitology
Naomi N. Kimenyi, Kelvin M. Kimenyi, Nelson O. Amugune, Merid N. Getahun
Summary: Different trypanosome species in Africa show varying levels of genetic connectivity and independent evolutionary origins based on microsatellite genotyping. Microsatellite markers have the potential to serve as diagnostic markers for T. brucei and Trypanosoma evansi isolates. Trypanosoma vivax isolates show clustering based on sampling location, while T. congolense isolates from different locations in Kenya do not show genetic separation.
Review
Microbiology
Mason H. Lee, Miguel Medina Munoz, Rita V. M. Rio
Summary: Tsetse flies play a significant role in the transmission of Trypanosoma parasites, which cause African trypanosomiases in humans and animals. Microbial symbiosis is crucial for supplementing nutrient deficiencies in these flies, but the mechanisms and regulation of metabolic exchanges between partners are not well understood. In this study, the roles of microRNAs and DNA methylation in maintaining insect-microbiota homeostasis are proposed and supported by empirical evidence. Understanding the coordination between microbiota and host activity may have applications in manipulating host health and developing innovative vector control strategies.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Entomology
Ruben Sancho, Ana Guillem-Amat, Elena Lopez-Errasquin, Lucas Sanchez, Felix Ortego, Pedro Hernandez-Crespo
Summary: This study analyzed Spanish wild medfly populations in areas where SIT is applied, and found genetic markers of Vienna GSS released individuals, suggesting the introgression of Vienna strain genes into natural populations.
JOURNAL OF PEST SCIENCE
(2021)
Article
Biology
Yusuke Ikegawa, Koichi Ito, Chihiro Himuro, Atsushi Honma
Summary: Research has shown that in different mating systems of insects, bisexual release is most effective for swarm-type mating systems, while for scramble-type mating systems, it depends on the difference in female searching ability between sterile and wild males. The net impact of sterile females depends on the difference in sexual performance between wild and sterile males.
JOURNAL OF THEORETICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
James J. Bull, Richard Gomulkiewicz
Summary: This study explores the potential resistance evolution in female discrimination against mating with sterile males in the context of sterile insect technique (SIT). The authors find that the evolution of female discrimination is generally too slow to halt population collapse when a constant-release implementation of SIT is used. However, periodic premature cessation of SIT or using a proportional-release protocol can allow discrimination to evolve and prevent extinction if discriminating genotypes exist in the population.
Article
Agronomy
Chao Chen, Robert L. Aldridge, Seth Gibson, Jedidiah Kline, Vindhya Aryaprema, Whitney Qualls, Rui-de Xue, Leigh Boardman, Kenneth J. Linthicum, Daniel A. Hahn
Summary: This study investigated the effects of ionizing radiation on the survival, longevity, mating behavior, and sterility of Ae. aegypti mosquitoes. The results showed that a radiation dose of 50 Gy rendered males 99% sterile while maintaining mating competitiveness against unirradiated males. Females were completely sterilized at 30 Gy.
PEST MANAGEMENT SCIENCE
(2023)
Article
Parasitology
Danilo O. Carvalho, Rachel Morreale, Steven Stenhouse, Daniel A. Hahn, Maylen Gomez, Aaron Lloyd, David Hoel
Summary: This study demonstrates the effectiveness of the sterile insect technique in suppressing the population of Aedes aegypti and identifies the biological parameters for the technique. The findings provide a foundation for the operational phase of mosquito population suppression.
PARASITES & VECTORS
(2022)
Article
Microbiology
Irina Bemba, Arsene Lenga, Herman Parfait Awono-Ambene, Christophe Antonio-Nkondjio
Summary: This study provides updated information on the distribution of tsetse fly populations and the circulation of trypanosome species in different active HAT foci in Congo. The results suggest a high risk of potential transmission of animal trypanosomes in these foci.
Article
Parasitology
Norbert Becker, Sophie Min Langentepe-Kong, Artin Tokatlian Rodriguez, Thin Thin Oo, Dirk Reichle, Renke Luehken, Jonas Schmidt-Chanasit, Peter Luethy, Arianna Puggioli, Romeo Bellini
Summary: This study shows that an integrated control program consisting of community participation, door-to-door intervention, and the sterile insect technique can effectively control the population of Aedes albopictus. The use of Bacillus thuringiensis israelensis-based fizzy tablets and water-dispersible granular formulation is the most effective intervention in reducing the population, while the mass release of sterile male mosquitoes also yields good results.
PARASITES & VECTORS
(2022)
Article
Entomology
Joshua Reger, Jacob A. Wenger, Gurreet Brar, Charles Burks, Houston Wilson
Summary: Research on using sterile insect technique (SIT) to control navel orangeworm found that mass-rearing conditions reduce flight capacity, while irradiation interacts with moths in a sex-specific manner.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fabrizio Balestrino, Jeremy Bouyer, Marc J. B. Vreysen, Eva Veronesi
Summary: Effective control strategies, such as the sterile insect technique, play a crucial role in preventing the spread of vector-borne diseases. However, current sex separation techniques for mosquito vectors are not completely effective, resulting in increased risk of disease transmission. This study examined the transmission of dengue and chikungunya viruses in two Aedes species exposed to irradiation, and found that irradiation did not impact transmission efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
H. Yamada, H. Maiga, C. Kraupa, W. Mamai, N. S. Bimbile Somda, A. Abrahim, T. Wallner, J. Bouyer
Summary: The success of the sterile insect technique (SIT) depends on the sterility and mating success of the factory-reared sterile males. The quality of these males can be improved by reducing stress factors encountered during rearing, handling, and irradiation. Reliable and standard irradiation protocols are necessary to achieve consistent sterility levels. Immobilization methods like chilling and nitrogen exposure are commonly used, but they have varying effects on the irradiation dose-response and male quality parameters. Chilling does not harm longevity and flight ability when carefully controlled, while irradiation in nitrogen reduces sterility induction. However, exposure to nitrogen negatively impacts flight ability. These findings will help in standardizing and optimizing irradiation protocols for SIT to control medically significant mosquito populations.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biology
Celia Lutrat, Roenick P. Olmo, Thierry Baldet, Jeremy Bouyer, Eric Marois
Summary: In this study, transgenic lines of Aedes albopictus expressing the Nix gene were established, and it was found that Nix is sufficient for full masculinization in these mosquitoes. Additionally, the transgenic strains with a fluorescence marker provide a valuable tool for automated sex sorting.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hamidou Maiga, Deng Lu, Wadaka Mamai, Nanwintoum Severin Bimbile Somda, Thomas Wallner, Mame Thierno Bakhoum, Odet Bueno Masso, Claudia Martina, Simran Singh Kotla, Hanano Yamada, Gustavo Salvador Herranz, Rafael Argiles Herrero, Chee Seng Chong, Cheong Huat Tan, Jeremy Bouyer
Summary: This study aims to standardize the use of a rapid quality control flight test device and provide affordable, practical quality control tools for successful implementation of the sterile insect technique. Factors such as tube color, lure and fan use, mosquito species, and mosquito age and density were found to significantly impact escape rates. A cheaper version of the device was also designed and validated.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Enkelejda Velo, Fabrizio Balestrino, Perparim Kadriaj, Danilo Oliveira Carvalho, Ahmadou Dicko, Romeo Bellini, Arianna Puggioli, Dusan Petric, Antonios Michaelakis, Francis Schaffner, David Almenar, Igor Pajovic, Alfred Beqirllari, Migel Ali, Gjergji Sino, Elton Rogozi, Vjola Jani, Adelina Nikolla, Tanja Porja, Thanas Goga, Elena Falcua, Mihaela Kavran, Dubravka Pudar, Ognyan Mikov, Nadya Ivanova-Aleksandrova, Aleksandar Cvetkovikj, Muhammet Mustafa Akiner, Rados Mikovic, Lindita Tafaj, Silva Bino, Jeremy Bouyer, Wadaka Mamai
Summary: This study conducted a mark-release-recapture experiment with sterilized Asian tiger mosquitoes in Albania to estimate their dispersal capacity and field performance. The experiment provided valuable data for decision-making and planning of pilot sterile insect technique trials. Additionally, the study found that both BG traps and human landing catches were effective methods for monitoring adult mosquitoes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Fabrizio Balestrino, Arianna Puggioli, Marco Malfacini, Alessandro Albieri, Marco Carrieri, Jeremy Bouyer, Romeo Bellini
Summary: Mark-release-recapture (MRR) trials were conducted in Northern Italy to evaluate the survival, dispersal, and mating capacity of radio-sterilized Aedes albopictus males. Environmental factors such as barriers, shading, and vegetation seemed to have a greater influence on the survival and dispersal rates than weather parameters. The study confirmed a linear relationship between the daily survival rate of sterile males and relative humidity, and observed different dispersal capacities in low- and high-vegetated areas. The quality of the released males was not affected by irradiation and marking procedures. The data estimated different population densities of A. albopictus in the study areas.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Nonhlanhla L. Ntoyi, Thabo Mashatola, Jeremy Bouyer, Carina Kraupa, Hamidou Maiga, Wadaka Mamai, Nanwintoum S. Bimbile-Somda, Thomas Wallner, Danilo O. Carvalho, Givemore Munhenga, Hanano Yamada
Summary: This study evaluated a mutant strain carrying a transgenic marker, which was crossed with a local strain in South Africa, as a reliable sexing tool.
Article
Multidisciplinary Sciences
Ronan Brouazin, Iris Claudel, Renaud Lancelot, Guillaume Dupuy, Louis-Clement Gouagna, Marlene Dupraz, Thierry Baldet, Jeremy Bouyer
Summary: This study provides insights into monitoring Aedes populations in La Reunion and suggests using black plastic ovitraps placed in vacoa trees for effective detection. The ovitraps located in the tree canopy showed higher detection and apparent density for Ae. aegypti, while Ae. albopictus showed a preference for blotting paper as the oviposition surface.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Nanwintoum Severin Bimbile Somda, Hanano Yamada, Carina Kraupa, Wadaka Mamai, Hamidou Maiga, Simran Singh Kotla, Thomas Wallner, Claudia Martina, Jeremy Bouyer
Summary: This study investigated the use of nitrogen in the irradiation of adult Aedes albopictus and Ae. aegypti. The results showed that nitrogen treatment improved the longevity of irradiated males but slightly reduced their flight ability. Nitrogen treatment was found to be a reliable method for adult mosquito immobilization.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Entomology
Hanano Yamada, Benewende Aristide Kabore, Nanwintoum Severin Bimbile Somda, Nonhlanhla L. L. Ntoyi, Chantel Janet de Beer, Jeremy Bouyer, Carlos Caceres, Robert L. L. Mach, Yeudiel Gomez-Simuta
Summary: The sterile insect technique (SIT) is an environmentally friendly and species-specific method of pest control that involves mass production of target insects in a factory and sterilizing them using irradiation, typically gamma rays. As gamma sources become more difficult and expensive to obtain, and regulations surrounding their use become stricter, there is growing interest in alternative irradiation methods such as X-rays. A recent study found that X-rays can be used effectively to sterilize major SIT target insect species, making X-ray units suitable for small-to-medium-sized SIT programs.
Article
Parasitology
Mahamat Hissene Mahamat, Adeline Segard, Jean-Baptiste Rayaisse, Rafael Argiles-Herrero, Andrew Gordon Parker, Philippe Solano, Adly Mohamed Mohamed Abd-Alla, Jeremy Bouyer, Sophie Ravel
Summary: Based on experimental data, the research found that sterile male tsetse flies cannot transmit Trypanosoma brucei brucei. The reliable evidence indicates that the release of large numbers of sterile male flies does not increase the potential risk of tsetse flies transmitting the parasite.
PARASITES & VECTORS
(2023)
Editorial Material
Parasitology
Jeremy Bouyer
Summary: Recent research by Evans et al. confirms compensation and overcompensation in Aedes mosquitoes under field conditions, where equal or increased densities of emerging adults may result from reduced larval densities. This article discusses the consequences of applying the sterile insect technique and provides recommendations to avoid counter-productive effects.
TRENDS IN PARASITOLOGY
(2023)
Article
Entomology
Hamidou Maiga, Mame Thierno Bakhoum, Wadaka Mamai, Gorgui Diouf, Nanwintoum Severin Bimbile Somda, Thomas Wallner, Claudia Martina, Simran Singh Kotla, Odet Bueno Masso, Hanano Yamada, Bazoumana B. D. Sow, Assane Gueye Fall, Jeremy Bouyer
Summary: This study developed and assessed a novel method for long-distance shipments of sterile male mosquitoes from the laboratory to the field. The new mass transport protocol allowed the successful shipment of 50,000 sterile male mosquitoes for up to four days, maintaining good survival rates and flight ability. This novel system can be used worldwide for shipping sterile males for journeys of two to four days.
Article
Biology
Celia Lutrat, Myriam Burckbuchler, Roenick Proveti Olmo, Remy Beugnon, Albin Fontaine, Omar S. S. Akbari, Rafael Argiles-Herrero, Thierry Baldet, Jeremy Bouyer, Eric Marois
Summary: Four new transgenic sexing strains have been developed to identify and sort male and female Aedes mosquitoes using fluorescence markers linked to the sex-determining loci. These strains provide a more efficient and cost-effective method for controlling disease vectoring mosquitoes. They enable faster and more accurate sex separation, allowing for the production of non-transgenic males. This technology can greatly enhance control programs against these important vectors.
COMMUNICATIONS BIOLOGY
(2023)
Article
Parasitology
Hanano Yamada, Hamidou Maiga, Carina Kraupa, Nanwintoum Severin Bimbile Somda, Wadaka Mamai, Thomas Wallner, Jeremy Bouyer
Summary: Balancing process efficiency and adult sterile male biological quality is crucial in the success of the sterile insect technique against insect pest populations. For SIT against mosquitoes, optimizing the irradiation step and considering dose fractionation can significantly improve male mating competitiveness. Fractionating the dose showed positive effects on male longevity and mating competitiveness, but the added labor in SIT programs for mosquito vector management may not outweigh the benefits.