Review
Cell Biology
Pengfei Lu, Yunzhe Lu
Summary: Epithelium is traditionally considered an immotile tissue, but recent research has shown that it is actually more motile than previously thought. Epithelial-mesenchymal transition (EMT) is a crucial process for embryonic development, organ formation, and cancer metastasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Jia Chen, Daniel St Johnston
Summary: This article reviews the current understanding of cell polarization in different stages (embryonic, larval, pupal, and adult) of Drosophila midgut development, and discusses the unresolved mechanisms that control changes in cell arrangements, cell shape, and cell polarity during midgut development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Vaishali Chakravarty, Libi Anandi, K. A. Ashiq, K. Abhijith, Rintu Umesh, Mayurika Lahiri
Summary: Lipid species have diverse biological functions based on structural differences, some of which interact with proteins as second messengers. The accumulation of phospholipid mediator PAF in the tumor microenvironment disrupts cellular homeostasis and increases metastatic potential.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Aiguo Tian, Xian-Feng Wang, Yuting Xu, Virginia Morejon, Yi-Chun Huang, Chidi Nwapuda, Wu-Min Deng
Summary: This study in Drosophila ovarian follicular epithelium reveals the role of specific protein complexes, growth factor signaling, and transcription factor Pointed in maintaining cell polarity and directional growth, providing insights into epithelial tumor formation.
Article
Biochemistry & Molecular Biology
Longxiyu Meng, Jie Gao, Wenjing Mo, Baojun Wang, Hongwei Shen, Wenmin Cao, Meng Ding, Wenli Diao, Wei Chen, Qing Zhang, Jiaxin Shu, Huiqi Dai, Hongqian Guo
Summary: This study reveals that MIOX is a crucial regulatory molecule in ccRCC, which impacts the growth, metastasis, and prognosis of the disease.
Article
Multidisciplinary Sciences
Xinyi Cao, Jiuchen Wang, Tianye Zhang, Zhiheng Liu, Lijun Liu, Ying Chen, Zehua Li, Youlu Zhao, Qi Yu, Tong Liu, Jing Nie, Yuanjie Niu, Yupeng Chen, Li Yang, Lirong Zhang
Summary: Renal tubular epithelial cells can respond to varying kidney injuries either adaptively or maladaptively. This study elucidates the chromatin-mediated mechanism underlying the responses of renal tubular epithelial cells to different kidney injuries, and identifies RXR alpha as a therapeutic target for acute kidney injury.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Qiwei Chen, Yue Kuai, Shujing Wang, Xinqing Zhu, Hongyu Wang, Wenlong Liu, Liang Cheng, Deyong Yang
Summary: This study developed an EMT gene signature and trained a deep convolutional neural network to classify ccRCC according to EMT subtypes, predict genomic data and prognosis. The study investigated the clinical significance and multiomics analysis of the EMT signature. The EMT subtypes were characterized by distinctive genomes, metabolic states, and immune components.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ruimei Zhou, Jiashun Liao, Dunpeng Cai, Qin Tian, Enping Huang, Tianming Lu, Shi-You Chen, Wei-Bing Xie
Summary: The study identified Nupr1 as a novel regulator promoting renal fibrosis, essential for fibroblast activation and/or epithelial-mesenchymal transition during renal fibrogenesis. Nupr1 mediated TGF-beta-induced myofibroblast activation and collagen synthesis by initiating Smad3 signaling pathway, and the Nupr1 inhibitor alleviated UUO-induced renal fibrosis.
Article
Medicine, Research & Experimental
Daniela Judith Romero, Lucila Gisele Pescio, Bruno Jaime Santacreu, Jazmin Maria Mosca, Norma Beatriz Sterin-Speziale, Nicolas Octavio Favale
Summary: Epithelial renal cells can adopt different cellular phenotypes through epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET). Sphingosine-1-phosphate (S1P) plays a crucial role in cell migration and differentiation, and its functions vary depending on the stage of cell differentiation and activation of S1P receptor 2 (S1PR2). Activation of S1PR2 promotes migration in fully differentiated cells but inhibits migration in non-differentiated cells. These findings provide new insights into the modulation of renal epithelial cell plasticity and have implications for research in various diseases.
Article
Biochemistry & Molecular Biology
Hua Fu, Yong-Hong Gu, Juan Tan, Ye-Ning Yang, Guo-Hui Wang
Summary: The crosstalk between macrophages and tubular epithelial cells is important in the progression of renal fibrosis. In this study, the significance of circular RNA ACTR2 (circACTR2) in regulating macrophage inflammation, epithelial-mesenchymal transition (EMT) of tubular epithelial cells, and the development of renal fibrosis was revealed. circACTR2 promoted the activation of NLRP3 inflammasome, inflammation in macrophages, and stimulated EMT and fibrosis of tubular epithelial cells by sponging miR-561. Knocking down circACTR2 and overexpressing miR-561 may benefit the treatment of renal fibrosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Sergio Simoes, Gerald Lerchbaumer, Milena Pellikka, Paraskevi Giannatou, Thomas Lam, Dohyun Kim, Jessica Yu, David ter Stal, Kenana Al Kakouni, Rodrigo Fernandez-Gonzalez, Ulrich Tepass
Summary: Most forms of cancer involve the movement of cells out of an epithelial layer, a process that requires the loss of apical polarity. This study reveals the regulatory role of key polarity proteins in this loss of polarity. The dynamic regulation of the apical Crumbs polarity complex is crucial for normal neural stem cell ingression.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Jiabao Feng, Fugui Zhu, Dan Ye, Qingquan Zhang, Xudong Guo, Changsheng Du, Jiuhong Kang
Summary: This study identifies Sin3a as a novel regulator in the mesenchymal-to-epithelial transition (MET) process during early reprogramming, where Sin3a functions as an epigenetic coactivator, cooperating with Tet1 to activate the epithelial program and promote somatic cell reprogramming initiation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
I-Hung Shao, Pei-Hua Peng, Heng-Hsiung Wu, Ji-Lin Chen, Joseph Chieh-Yu Lai, Jeng-Shou Chang, Han-Tsang Wu, Kou-Juey Wu, See-Tong Pang, Kai-Wen Hsu
Summary: In this study, a hypoxia-induced long non-coding RNA (lncRNA) RP11-367G18.1 was identified to be overexpressed in clear cell renal cell carcinoma (ccRCC) tissues. RP11-367G18.1 interacted with histone acetyltransferase p300 and regulated lysine 16 acetylation on histone 4 (H4K16Ac), thereby promoting ccRCC metastasis and growth. RP11-367G18.1 was associated with poor prognosis and may serve as a therapeutic target for ccRCC.
Article
Oncology
Pelin Balcik-Ercin, Laure Cayrefourcq, Rama Soundararajan, Sendurai A. Mani, Catherine Alix-Panabieres
Summary: Metastasis is a complex and not fully understood process in cancer, where circulating tumor cells (CTCs) play a crucial role. CTCs possess a unique ability to transition between epithelial and mesenchymal states, known as epithelial-to-mesenchymal plasticity (EMP), which aids in their metastatic potential. This study focuses on the EMP phenotype of colon CTC lines, showing that these cells have minimal mesenchymal features but exhibit an increase in mesenchymal-epithelial transition (MET) markers, suggesting a quick reversion to the epithelial state for successful metastasis.
Article
Biotechnology & Applied Microbiology
You-Peng Zhang, Yong-Biao Cheng, Sen Li, Ning Zhao, Zhao-Hui Zhu
Summary: A new survival signature based on EMT-related lncRNAs for renal clear cell carcinoma was constructed, with one lncRNA identified as an independent predictor of prognosis and novel markers for ccRCC prognosis were identified. The underlying mechanism between EMT-related lncRNAs in ccRCC and tumor immunity requires further study.
Article
Cell Biology
Silvia Chafino, Enric Urena, Jordi Casanova, Elena Casacuberta, Xavier Franch-Marro, David Martin
Review
Developmental Biology
Sonja Nowotschin, Anna-Katerina Hadjantonakis, Kyra Campbell
Article
Multidisciplinary Sciences
Kyra Campbell, Fabrizio Rossi, Jamie Adams, Ioanna Pitsidianaki, Francisco M. Barriga, Laura Garcia-Gerique, Eduard Batlle, Jordi Casanova, Andreu Casali
NATURE COMMUNICATIONS
(2019)
Review
Cell Biology
Jing Yang, Parker Antin, Geert Berx, Cedric Blanpain, Thomas Brabletz, Marianne Bronner, Kyra Campbell, Amparo Cano, Jordi Casanova, Gerhard Christofori, Shoukat Dedhar, Rik Derynck, Heide L. Ford, Jonas Fuxe, Antonio Garcia de Herreros, Gregory J. Goodall, Anna-Katerina Hadjantonakis, Ruby J. Y. Huang, Chaya Kalcheim, Raghu Kalluri, Yibin Kang, Yeesim Khew-Goodall, Herbert Levine, Jinsong Liu, Gregory D. Longmore, Sendurai A. Mani, Joan Massague, Roberto Mayor, David McClay, Keith E. Mostov, Donald F. Newgreen, M. Angela Nieto, Alain Puisieux, Raymond Runyan, Pierre Savagner, Ben Stanger, Marc P. Stemmler, Yoshiko Takahashi, Masatoshi Takeichi, Eric Theveneau, Jean Paul Thiery, Erik W. Thompson, Robert A. Weinberg, Elizabeth D. Williams, Jianhua Xing, Binhua P. Zhou, Guojun Sheng
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2020)
Editorial Material
Biology
Kyra Campbell, Emily S. Noel, Alexander G. Fletcher, Natalia A. Bulgakova
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Review
Biology
Andrew T. Plygawko, Shohei Kan, Kyra Campbell
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Review
Anatomy & Morphology
Sven Jonckheere, Jamie Adams, Dominic De Groote, Kyra Campbell, Geert Berx, Steven Goossens
Summary: Metastasis, the spread of cancer cells to distant sites, is the primary cause of cancer morbidity and mortality. During the initial stages of metastasis, cancer cells undergo EMT, becoming migratory and invasive mesenchymal-like cells with stem cell properties. Targeting EMT in therapy has the potential to prevent cancer cell invasion and dissemination, reduce cancer stemness, and enhance the effectiveness of chemotherapy.
CELLS TISSUES ORGANS
(2022)
Article
Genetics & Heredity
Panagiotis Giannios, Jordi Casanova
Summary: During the development of holometabolous insects like Drosophila, specific larval cells known as Adult Progenitor Cells (APCs) survive metamorphosis and differentiate into adult structures with the help of the headcase (hdc) gene. Hdc plays a dual role in systemic hormone control and local tissue growth suppression in APC clusters, contributing to their survival and response to stress. The evolutionary conserved nature of hdc suggests potential implications for human cancers.
Article
Biochemistry & Molecular Biology
Juan J. Fraire-Zamora, Sebastien Tosi, Jerome Solon, Jordi Casanova
Summary: This study investigates how hormone-driven extracellular matrix remodeling controls tissue shortening and apoptotic cell removal through the transcriptional activity of Yki, leading to organ disassembly during animal development.
Correction
Cell Biology
Jing Yang, Parker Antin, Geert Berx, Cedric Blanpain, Thomas Brabletz, Marianne Bronner, Kyra Campbell, Amparo Cano, Jordi Casanova, Gerhard Christofori, Shoukat Dedhar, Rik Derynck, Heide L. Ford, Jonas Fuxe, Antonio Garcia de Herreros, Gregory J. Goodall, Anna-Katerina Hadjantonakis, Ruby Y. J. Huang, Chaya Kalcheim, Raghu Kalluri, Yibin Kang, Yeesim Khew-Goodall, Herbert Levine, Jinsong Liu, Gregory D. Longmore, Sendurai A. Mani, Joan Massague, Roberto Mayor, David McClay, Keith E. Mostov, Donald F. Newgreen, M. Angela Nieto, Alain Puisieux, Raymond Runyan, Pierre Savagner, Ben Stanger, Marc P. Stemmler, Yoshiko Takahashi, Masatoshi Takeichi, Eric Theveneau, Jean Paul Thiery, Erik W. Thompson, Robert A. Weinberg, Elizabeth D. Williams, Jianhua Xing, Binhua P. Zhou, Guojun Sheng
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Article
Biology
Silvia Chafino, Panagiotis Giannios, Jordi Casanova, David Martin, Xavier Franch-Marro
Summary: During development, organisms go through different morphological stages, such as larva, pupa, and adult, and the sequential expression of transcription factors chinmo, Br-C, and E93 play a crucial role in determining the identity of these stages. Chinmo promotes growth in larval and imaginal tissues, while Br-C and E93 act as tumor suppressors. The role of chinmo as a juvenile specifier is conserved in hemimetabolous insects.
Article
Biochemistry & Molecular Biology
Eva Parisi, Ivan Hidalgo, Robert Montal, Ona Pallise, Jordi Tarragona, Anabel Sorolla, Anna Novell, Kyra Campbell, Maria Alba Sorolla, Andreu Casali, Antonieta Salud
Summary: In this study, a genetic screen identified 13 new genes that modified circulating tumor cell numbers in a whole animal CRC model. Evaluation of candidate genes at the gene expression level in both internal and independent human CRC cohorts revealed that low expression of PLA2G12A correlated with poor clinical outcomes. Further analysis showed that low expression of PLA2G12A was enriched in epithelial-mesenchymal transition signatures. Functional validation using colon cancer cell lines demonstrated that PLA2G12A deficiency increased cell proliferation, migration, and invasion. Overall, this study identifies PLA2G12A as a prognostic biomarker for early-stage CRC and provides evidence for its role in tumor growth and dissemination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ioanna Pitsidianaki, Jason Morgan, Jamie Adams, Kyra Campbell
Summary: The study reveals that Mesenchymal-to-epithelial transition (MET) during midgut morphogenesis in Drosophila melanogaster involves down-regulation of the EMT transcription factor Serpent, secretion of specific laminins via the CopII secretory pathway, and basal polarization of alpha PS1 integrin. These mechanisms are crucial for the formation and positioning of the midgut epithelium, and targeting these pathways along with therapies preventing EMT may provide a strategy to block metastasis in cancer.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sandra Casani, Jordi Casanova, Marta Llimargas