Journal
MEAT SCIENCE
Volume 85, Issue 2, Pages 302-305Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2010.01.018
Keywords
Capillary electrophoresis; Electrochemical detection; beta-Agonises
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Funding
- Basic Research Fund of the Science and Technology Commission of Shanghai Municipality [0DZ05125, 09ZR1409700]
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The fast separation capability of a capillary electrophoresis with electrochemical detection (CE-ED) system was demonstrated by determining five beta-agonists including clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in several real samples. Factors influencing the separation and detection were examined and optimized. Under the optimum conditions, the five beta-agonists could be well separated within 15 min at a separation voltage of 14 kV in a 100 mmoL H3BO3-Na2B4O7 running buffer (pH 9.0). This method was successfully used in the analysis of clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in pig feed, pig urine and pig liver, providing a useful method for monitoring the illegal use of beta-agonists from growth promotion in food-producing animals. (C) 2010 Elsevier Ltd. All rights reserved.
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