Journal
MEAT SCIENCE
Volume 83, Issue 3, Pages 523-527Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2009.06.037
Keywords
Listeria monocytogenes; Linguica; Pork meat; Risk assessment
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [07/51361-2, 07/54891-2]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordena do de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
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Linguica is a highly popular and appreciated pork product in Brazil, frequently consumed undercooked. Aiming at collection of data for a future risk assessment, this study evaluated the prevalence and counts of Listeria monocytogenes in linguica samples collected at retail level in Sao Paulo, SP, Brazil. ISO methods were used for detection and enumeration of the pathogen (11290-1 and 11290-2, respectively). Isolates were submitted to Simplex-PCR for hlyA gene and those with biochemical features of L. monocytogenes and hlyA positive were serotyped using a Multiplex PCR. Ninety percent of the samples were positive for Listeria spp., and L monocytogenes was detected in 42% of the samples, with counts below 10(2) CFU/g in all samples. A prevalence of uncommon serotypes 4a and 4c was observed. (C) 2009 Elsevier Ltd. All rights reserved.
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