Journal
MATRIX BIOLOGY
Volume 74, Issue -, Pages 121-132Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.matbio.2018.08.001
Keywords
Hypoxia; ischemic stroke; Middle cerebral artery occlusion; Neural plasticity; Structured illumination microscopy; Superresolution microscopy; Extracellular matrix
Categories
Funding
- German Research Council [He 3173/3-1, He 3173/11-1, SFB 642 TP A24, Fa 159/22-1]
- International Graduate School of Neuroscience (IGSN), Ruhr-University Bochum
Ask authors/readers for more resources
Despite the crucial role of perineuronal nets (PNNs) in neural plasticity and neurological disorders, their ultrastructural organization remains largely unresolved. We have developed a novel approach combining superresolution structured illumination microscopy (SR-SIM) and mathematical reconstruction that allows for quantitative analysis of PNN topology. Since perineuronal matrix is capable to restrict neural plasticity but at the same time is necessary to maintain synapses, we hypothesized that a beneficial post stroke recovery requires a reversible loosening of PNNs. Our results indicated that focal cerebral ischemia induces partial depletion of PNNs and that mild hypoperfusion not associated with ischemic injury can induce ultra-structural rearrangements in visually intact meshworks. In line with the activation of neural plasticity under mild stress stimuli, we provide evidence that topological conversion of PNNs can support post stroke neural rewiring. (C) 2018 The Authors. Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available