Article
Pharmacology & Pharmacy
Jingming Zhou, Yanghui Li, Zhanxiang Liu, Wenjing Qian, Yumei Chen, Yanhua Qi, Aiping Wang
Summary: The study successfully induced mice to produce specific antibodies against ZEN by screening six mimotopes, demonstrating the immunogenicity of these displayed peptides.
Review
Medical Laboratory Technology
V. E. R. O. N. I. C. A. VILLANUEVA, X. I. A. O. B. O. LI, J. I. M. E. N. E. Z. H. A. F. E. E. Z. M. FARIDI, V. I. N. E. E. T. GUPTA
Summary: This article discusses the role of CD11b on leukocytes in lupus nephritis (LN) and emphasizes the importance of targeting integrin CD11b as a therapeutic approach for systemic lupus erythematosus (SLE) and LN.
TRANSLATIONAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Ivan V. Panayotov, Attila G. Vegh, Marta Martin, Boyan Vladimirov, Christian Larroque, Csilla Gergely, Frederic J. G. Cuisinier, Elias Estephan
Summary: The functionalization of titanium and titanium alloys implant surfaces via material-specific peptides influences host/biomaterial interaction. The use of peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported. In vivo and in vitro experiments demonstrate the promising performance of bioengineered peptides in improving epithelial cell adhesion to titanium-based implants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Ya-Ru Li, Xiu-Xiu Dong, Wei-Yi Cai, Yi-Fan Liang, Xiao-Hui Chen, Xiang-Mei Li, Yu Wang, Ying-Ju Liu, Hong-Tao Lei, Zhen-Lin Xu
Summary: In this work, a monoclonal antibody (mAb) with good affinity and high selectivity against TTX was fabricated, and a phage-displayed mimotope with specific binding to the anti-TTX mAb was selected. Based on the mAb and mimotope, a non-toxic and dual-mode immunosensor (electrochemical and colorimetric signal) was developed for the highly efficient and sensitive detection of TTX.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Nanoscience & Nanotechnology
Ji Hong Kim, Hye-Seon Jeong, Jaehyeon Hwang, Dae-Hyuk Kweon, Chang-Hyung Choi, Jong Pil Park
Summary: A sensor based on affinity peptide-immobilized hydrogel microspheres has been developed for the selective detection of influenza A virus. The sensor shows rapid and accurate detection capability, which is crucial for the effective control of pandemic flu outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Immunology
Ahmad Najafi, Reza Valadan, Hossein Asgarian-Omran, Alireza Rafiei, Mohsen Tehrani
Summary: In this study, a recombinant phage was generated using the phage display method to select monoclonal phages that can react with the dimerization domain of HER2 receptor. Functional tests confirmed their specificity and anti-tumor properties.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hawk Leong Yuen, Szn Yi Chan, Yi En Ding, Suxing Lim, Gim Cheong Tan, Chiew Ling Kho
Summary: This study aimed to develop a potent antibacterial peptide (ABP) with minimal toxicity through phage display selection and computer-assisted modification. A peptide called PAM-5 with enhanced antibacterial features was obtained by modifying the peptide selected for its high affinity to Pseudomonas aeruginosa cell surface using an online peptide database. PAM-5 showed activity against various Gram-negative and selected Gram-positive bacteria, and was stable in human plasma without toxic effects on human cells and erythrocytes.
Review
Microbiology
Weronika Jaroszewicz, Joanna Morcinek-Orlowska, Karolina Pierzynowska, Lidia Gaffke, Grzegorz Wegrzyn
Summary: Phage display technology is a widely applicable method in biotechnology, recognized by the Nobel Prize. This article provides an overview of the technology, including comparisons of different display systems, their advantages and disadvantages, and examples of applications in various fields. It also discusses other peptide display technologies and highlights the ongoing development and improvements in these methods, offering new solutions in the future.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Ruiqi Huang, Gabrielle Warner Jenkins, Yunjeong Kim, Robyn L. Stanfield, Amrinder Singh, Maria Martinez-Yamout, Gerard J. Kroon, Jonathan L. Torres, Abigail M. Jackson, Abigail Kelley, Namir Shaabani, Baisen Zeng, Michael Bacica, Wen Chen, Christopher Warner, Jasmina Radoicic, Joongho Joh, Krishani Dinali Perera, Huldah Sang, Tae Kim, Jianxiu Yao, Fangzhu Zhao, Devin Sokd, Dennis R. Burton, Jeff Allen, William Harriman, Waithaka Mwangi, Donghoon Chung, John R. Teijaro, Andrew B. Ward, H. Jane Dyson, Peter E. Wright, Ian A. Wilson, Kyeong-Ok Chang, Duncan Mcgregor, Vaughn V. Smider
Summary: Antibodies derived from cows have the potential to be a new class of therapeutics, capable of neutralizing various SARS-CoV-2 variants and providing protection against disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Chemistry, Analytical
Ping Xu, Subhadeep Ghosh, Anam Rana Gul, Jigna R. Bhamore, Jong Pil Park, Tae Jung Park
Summary: Biosensors have seen rapid growth in the field of biological detection and biomedical diagnosis, with displayed peptides from phage display libraries being recognized as a promising alternative to antibodies. The advantages of displayed peptides include high affinity, low cost, ease of design and modification, mass production, and increased resistance to complex environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Techit Thavorasak, Monrat Chulanetra, Kittirat Glab-ampai, Kodchakorn Mahasongkram, Nawannaporn Sae-lim, Karsidete Teeranitayatarn, Thaweesak Songserm, Rungrueang Yodsheewan, Dachrit Nilubol, Wanpen Chaicumpa, Nitat Sookrung
Summary: Porcine epidemic diarrhea virus (PEDV) is a highly contagious pathogen that causes a severe enteric disease in pigs. Understanding the enhancing epitope of PEDV protein can contribute to the development of a safer and more effective vaccine.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Tianyang You, Yuan Ding, He Chen, Guangyue Song, Lianrun Huang, Minghua Wang, Xiude Hua
Summary: Competitive and noncompetitive immunoassays for clothianidin based on phage-displayed peptides were developed to improve sensitivity and specificity. The noncompetitive method showed the best specificity and sensitivity for clothianidin, with no cross-reactivity with analogs. The results demonstrated good recoveries and consistency with high-performance liquid chromatography in the analysis of blind samples.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Claire Milsted, Bo Dai, Nelson Garcia, Lu Yin, Yan He, Shahryar Kianian, Wojciech Pawlowski, Changbin Chen
Summary: This study used phage display to investigate the interactions of maize RAD51A1 with other proteins. The results revealed several peptide sequences that bind to ZmRAD51A1, supporting a potential role for ZmRAD51A1 in transcriptional regulation and plant defense.
Article
Agriculture, Dairy & Animal Science
Pakpoom Navanukraw, Sroisuda Chotimanukul, Taratorn Kemthong, Kiattawee Choowongkomon, Kaywalee Chatdarong
Summary: This study developed a novel form of contraception for long-tailed macaques by reducing sperm output without altering testosterone levels. It offers promising insights into the development of new methods of contraception for long-tailed macaques.