Journal
MATERIALS SCIENCE AND TECHNOLOGY
Volume 26, Issue 11, Pages 1292-1297Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1179/026708310X12798718274070
Keywords
Electrospinning; Plasma treatment; Protein absorption; Protein immobilisation; Cell adhesion
Funding
- Integrated Nanotechnology Research Center, Khon Kaen University
- National Nanotechnology Center, National Science and Technology Development Agency, Ministry of Science and Technology, Thailand through Center of Excellence
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Nanofibres fabricated from synthetic polymers via electrospinnning may be uniform and possess consistent quality; however, due to the nature of polymers, these nanofibres may not serve as suitable substrates for cell adhesion. Thus, the present work is aimed to enhance cell adherence onto the as spun poly(e-caprolactone) (PCL) scaffolds via simple protein absorption, air plasma treatment and protein immobilisation methods. The as spun PCL fibres had average fibre diameter of 475.99 +/- 194.52 nm with average porosity of 72.29 +/- 1.90%. The laminin absorbed scaffold exhibited 20% increase in cell adhesion compared to the original scaffold. With the plasma treatment, the scaffolds increased their fibre sizes, but no significant change occurred in their porosities. The as spun PCL scaffold treated with air plasma was less hydrophobic and exhibited 66% increase in cell adherence compared to the original scaffold. When laminin protein was also included, a greater increase in cell adhesion was observed (84%). Comparing all methods, a laminin immobilised scaffold, with argon/oxygen plasma treatment followed by protein grafting with acrylic acid, showed the highest result of cell adhesion (96%). The results of the present work demonstrate the comparison of various means of using plasma treatment and laminin protein to enhance cell adherence onto the as spun PCL scaffolds, making it more suitable for tissue engineering and wound dressing applications.
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