Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 36, Issue -, Pages 25-32Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2013.11.035
Keywords
Glass hollow fibers; Hydrogel; Growth factor release; Nerve guidance channel; In vitro test
Categories
Funding
- BICONERVE project (Regional Project, Regione Piemonte)
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A novel system for the release of neurotrophic factor into a nerve guidance channel (NGC) based on resorbable phosphate glass hollow fibers (50P(2)O(5)-30CaO-9Na(2)O-3SiO(2)-3MgO-2.5K(2)O-2.5TiO(2) mol%) in combination with a genipin-crosslinked agar/gelatin hydrogel (A/G_GP) is proposed. No negative effect on the growth of neonatal olfactory bulb ensheathing cell line (NOBEC) as well as on the expression of pro- and anti-apoptotic proteins was measured in vitro in the presence of fiber dissolution products in the culture medium. For the release studies, fluorescein isothiocyanate-dextran (FD-20), taken as growth factor model molecule, was solubilized in different media and introduced into the fiber lumen exploiting the capillary action. The fibers were filled with i) FD-20/phosphate buffered saline (PBS) solution, ii) FD-20/hydrogel solution before gelation and iii) hydrogel before gelation, subsequently lyophilized and then filled with the FD-20/PBS solution. The different strategies used for the loading of the FD-20 into the fibers resulted in different release kinetics. A slower release was observed with the use of A/G_GP hydrogel. At last, poly(epsilon-caprolactone) (PCL) nerve guides containing the hollow fibers and the hydrogel have been fabricated. (C) 2013 Elsevier B.V. All rights reserved.
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